scholarly journals Relationship between Soy Sauce Color Intensity and Xylanolytic Enzyme Activity of Soy Sauce Koji Mold, Aspergillus oryzae

2002 ◽  
Vol 97 (3) ◽  
pp. 196-203
Author(s):  
Tadaaki HASHIMOTO ◽  
Yoshiyuki NAKATA ◽  
Kiyoshi ITO
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Oryzae ◽  
Soy Sauce ◽  
Color Intensity ◽  
Koji Mold ◽  
Xylanolytic Enzyme

Improvement of the quality of soy sauce by reducing enzyme activity in Aspergillus oryzae

2019 ◽  
Vol 292 ◽  
pp. 81-89 ◽  
Author(s):  
Chengfang Ding ◽  
Meng Meng ◽  
Yuyang Jiang ◽  
Lihua Hou
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Oryzae ◽  
Soy Sauce

Sequence Analysis, Overexpression, and Antisense Inhibition of a β-Xylosidase Gene, xylA, from Aspergillus oryzae KBN616

1999 ◽  
Vol 65 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Noriyuki Kitamoto ◽  
Shoko Yoshino ◽  
Kunio Ohmiya ◽  
Norihiro Tsukagoshi
Keyword(s):  
Aspergillus Oryzae ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Soy Sauce ◽  
Host Strain ◽  
Partial Length ◽  
Glycosylation Sites ◽  
Key Enzyme ◽  
Koji Mold

ABSTRACT β-Xylosidase secreted by the shoyu koji mold, Aspergillus oryzae, is the key enzyme responsible for browning of soy sauce. To investigate the role of β-xylosidase in the brown color formation, a major β-xylosidase, XylA, and its encoding gene were characterized. β-Xylosidase XylA was purified to homogeneity from culture filtrates of A. oryzae KBN616. The optimum pH and temperature of the enzyme were found to be 4.0 and 60°C, respectively, and the molecular mass was estimated to be 110 kDa based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The xylA gene comprises 2,397 bp with no introns and encodes a protein consisting of 798 amino acids (86,475 Da) with 14 potential N-glycosylation sites. The deduced amino acid sequence shows high similarity toAspergillus nidulans XlnD (70%), Aspergillus niger XlnD (64%), and Trichoderma reesei BxII (63%). The xylA gene was overexpressed under control of the strong and constitutive A. oryzae TEF1 promoter. One of the A. oryzae transformants produced approximately 13 times more of the enzyme than did the host strain. The partial-length antisense xylA gene expressed under control of the A. oryzae TEF1 promoter decreased the β-xylosidase level inA. oryzae to about 20% of that of the host strain.

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