scholarly journals Izozyme polymorphism of alpha-amylase in soft wheat

2019 ◽  
Vol 24 ◽  
pp. 160-165
Author(s):  
O. M. Radchenko ◽  
N. V. Sandetska ◽  
M. O. Dykun ◽  
L. V. Sirant

Aim. Study of alpha amylase polymorphism in winter wheat varieties. Methods. Isoenzymes of alpha-amylase were detected by electrophoretic protein separation in a polyacrylamide gel. The fall number on the Falling Number 1700 was determined. Results. Strains from various collections were explored: Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Kiev, The Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation of the National Academy of Agricultural Sciences of Ukraine, Odesa and german strains. The polymorphism of wheat grain alpha-amylase was detected by the method of electrophoretic protein separation. We searched associations between variants of alpha-amylase and resistance to pre-harvest germination, the falling number in the examined samples was determined. Conclusions. The frequency of the prevalence of variants for isoenzymes of alpha-amylase in soft wheat varieties was estimated. It was shown that genotypes containing the variant of the isoenzymes АbC are more resistant to pre-harvest germination. Among the studied varieties of PBGI NAAS and IPPG NAS of Ukraine, the variant of alpha-amylase AbC. Keywords: α-amylase, isoenzymes, soft wheat, electrophoresis, falling number.

2018 ◽  
Vol 22 ◽  
pp. 186-190
Author(s):  
O. M. Radchenko ◽  
L. V. Sirant ◽  
M. O. Dykun

Aim. Study of polymorphism of alpha amylase in winter wheat varieties. Methods. Isoenzymes of alpha-amylase were detected by electrophoretic protein separation in a polyacrylamide gel. The germination index (GI) was calculated by Walker-Simmons: GI = (7xn1 + 6xn2 + ... + 1xn7) / 7 x N, where n1, n2, ..., n7 is the number of seeds sprouted on the first, second, and further days to the seventh day, respectively, N – the total number of grains. Results. Varieties were explored: Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Kiev, The Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation of the National Academy of Agricultural Sciences of Ukraine, Odesa and german varieties.The polymorphism of wheat grain alpha-amylase was detected by the method of electrophoretic protein separation. We searched associations between variants of alpha-amylase and resistance to pre-harvest germination, the germination index in the studied samples was determined. Conclusions. The frequency of the prevalence of variants for isoenzymes of alpha-amylase in soft wheat varieties was estimated. It was shown that genotypes containing the variant of the isoenzymes АbC are more resistant to pre-harvest germination. Among the studied varieties of PBGI NAAS and IPPG NAS of Ukraine, the variant of alpha-amylase AbC. Keywords: α-amylase, soft wheat, electrophoresis, varieties.


1996 ◽  
pp. 4-15
Author(s):  
S. Golovaschenko ◽  
Petro Kosuha

The report is based on the first results of the study "The History of the Evangelical Christians-Baptists in Ukraine", carried out in 1994-1996 by the joint efforts of the Department of Religious Studies at the Institute of Philosophy of the National Academy of Sciences of Ukraine and the Odessa Theological Seminary of Evangelical Christian Baptists. A large-scale description and research of archival sources on the history of evangelical movements in our country gave the first experience of fruitful cooperation between secular and church researchers.


1996 ◽  
pp. 45-46
Author(s):  
Borys Lobovyk

"PHENOMENON OF RELIGION" - under this name on June 20-21, 1996, the All-Ukrainian Colloquium, convened by the Department of Religious Studies and the Ukrainian Association of Religious Studies took place at the Institute of Philosophy of the National Academy of Sciences of Ukraine. The purpose of the colloquium is to discuss the topical issues of Ukrainian religious studies concerning the nature, essence and functionality of religion as a social and historical phenomenon.


1998 ◽  
pp. 39-41
Author(s):  
Oleksandr N. Sagan

On April 22, 1998, on the occasion of the 65th anniversary of the publication of Arsen Rychinsky's "Problems of Ukrainian Religious Consciousness" in Kremenets (Ternopil region), the First Rychinsky reading was held. The organizers of the conference were: Ukrainian Association of Religious Studies, Ternopil Medical Academy named after them. I.Gorbachevsky, Department of Religious Studies at the Institute of Philosophy named after G.S. Skovoroda, National Academy of Sciences of Ukraine, Kremenets Medical College.


1997 ◽  
pp. 65-66
Author(s):  
V. Klymov

Under this name, on November 20-21, the All-Ukrainian Scientific and Practical Conference took place in Poltava, which became one of the many events devoted to the 2000th anniversary of the Nativity of Christ. Its organizers were Poltava Regional State Administration, Department of Religious Studies at the Institute of Philosophy named after G. Skovoroda, National Academy of Sciences of Ukraine, Poltava State Pedagogical Institute. VG Korolenko. The conference was attended by scholars: religious scholars, historians, philosophers, ethnographers, cultural experts, teachers from Kyiv, and many regions of Ukraine.


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