scholarly journals Downregulation of low-density lipoprotein receptor mRNA in lymphatic endothelial cells impairs lymphatic function through changes in intracellular lipids

Theranostics ◽  
2022 ◽  
Vol 12 (3) ◽  
pp. 1440-1458
Author(s):  
Laurent Vachon ◽  
Ali Smaani ◽  
Nolwenn Tessier ◽  
Gabriel Jean ◽  
Annie Demers ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Lymphatic Endothelial Cells ◽  
Lymphatic Function ◽  
Intracellular Lipids ◽  
Receptor Mrna ◽  
Density Lipoprotein Receptor

Upregulation of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in endothelial cells by nitric oxide deficiency

2004 ◽  
Vol 287 (1) ◽  
pp. F25-F32 ◽  
Author(s):  
I. V. Smirnova ◽  
T. Sawamura ◽  
M. S. Goligorsky
Keyword(s):  
Nitric Oxide ◽  
Renal Failure ◽  
Endothelial Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Common Denominator ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Density Lipoprotein Receptor

Endothelial cell dysfunction (ECD) is emerging as a common denominator for diverse cardiovascular abnormalities associated with inhibition of endothelial nitric oxide (NO) synthase (eNOS). Elevated levels of asymmetric dimethylarginine (ADMA), a potent eNOS inhibitor, are common in renal failure and may contribute to ECD. Through DNA microarray screening of genes modulated in human umbilical vein endothelial cells (HUVEC) by NG-nitro-l-arginine methyl ester (l-NAME), we found a 1.8-fold increase in low-density lipoprotein receptor-1 (LOX-1) expression. LOX-1 is a major endothelial receptor for oxidized low-density lipoproteins (OxLDL) and is assumed to play a role in the initiation and progression of atherosclerosis. Here, we confirmed the upregulation of LOX-1 mRNA and protein level by quantitative RT-PCR and Western blot analysis. Increased expression of LOX-1 was associated with the accumulation of DiI-labeled OxLDL (DiI-OxLDL) in ADMA- and l-NAME-pretreated HUVEC. To evaluate the contribution of LOX-1 in ADMA-induced accumulation of OxLDL by HUVEC, we used the competitive receptor inhibitor, soluble LOX-1. Treatment of HUVEC with soluble LOX-1 was associated with an approximately two- to threefold inhibition of DiI-OxLDL uptake in l-NAME- or ADMA-treated HUVEC. In conclusion, ADMA- or l-NAME-induced NO deficiency leads to the increased expression of LOX-1 mRNA and protein in HUVEC, which in turn results in the accumulation of OxLDL. Competition with LOX-1-soluble extracellular domain reduces OxLDL accumulation. In summary, elevated ADMA levels, i.e., in patients with renal failure, may be responsible for endothelial accumulation of OxLDL via upregulated LOX-1 receptor, thus contributing to endothelial lipidosis and dysfunction.

scholarly journals Elevation of low density lipoprotein-receptor mRNA concentration in human hepatoma HepG2 cells by macrolide antibiotics.

1993 ◽  
Vol 46 (5) ◽  
pp. 866-868 ◽  
Author(s):  
MASAMI OGASAWARA ◽  
NOBUAKI NARUSE ◽  
ATSUKO YOSHIMURA ◽  
YASUTARO HAMAGISHI ◽  
TOSHIKAZU OKI
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Human Hepatoma ◽  
Receptor Mrna ◽  
Mrna Concentration ◽  
Density Lipoprotein Receptor ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

scholarly journals Rat liver sinusoidal endothelial cells (LSECs) express functional low density lipoprotein receptor-related protein-1 (LRP-1)

2011 ◽  
Vol 55 (6) ◽  
pp. 1346-1352 ◽  
Author(s):  
Cristina Ionica Øie ◽  
Rupa Shree Appa ◽  
Ida Hilden ◽  
Helle Heibroch Petersen ◽  
Albrecht Gruhler ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Rat Liver ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Related Protein ◽  
Liver Sinusoidal Endothelial Cells ◽  
Density Lipoprotein Receptor ◽  
Lipoprotein Receptor Related Protein

scholarly journals cDNA cloning of the bovine low density lipoprotein receptor: feedback regulation of a receptor mRNA.

1983 ◽  
Vol 80 (24) ◽  
pp. 7501-7505 ◽  
Author(s):  
D. W. Russell ◽  
T. Yamamoto ◽  
W. J. Schneider ◽  
C. J. Slaughter ◽  
M. S. Brown ◽  
...  
Keyword(s):  
Cdna Cloning ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Feedback Regulation ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Receptor Mrna ◽  
Density Lipoprotein Receptor
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