scholarly journals The effect of physiological concentrations of bile acids on in vitro growth of Mycobacterium tuberculosis

2012 ◽  
Vol 102 (6) ◽  
pp. 522
Author(s):  
David Epstein ◽  
Kiki Mistry ◽  
Andrew Whitelaw ◽  
Gill Watermeyer ◽  
Keith Pettengell
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Bile Acids ◽  
In Vitro Growth

scholarly journals Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo

2002 ◽  
Vol 70 (5) ◽  
pp. 2566-2575 ◽  
Author(s):  
J. Reid Schwebach ◽  
Aharona Glatman-Freedman ◽  
Leslie Gunther-Cummins ◽  
Zhongdong Dai ◽  
John B. Robbins ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Envelope ◽  
Tween 80 ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vitro Growth ◽  
Comprehensive Picture ◽  
The Media ◽  
Surface Polysaccharide

ABSTRACT The outermost layer of Mycobacterium tuberculosis is composed primarily of two polysaccharides, glucan (GC) and arabinomannan. To analyze the surface polysaccharide composition of M. tuberculosis, we generated a monoclonal antibody (MAb) that binds M. tuberculosis GC and is known as MAb 24c5. Immunofluorescence and whole-mount immunoelectron microscopy indicated that GC is on the outermost portion of the bacteria. M. tuberculosis strains Erdman and CDC 1551 were analyzed for their ability to bind MAb 24c5 after in vitro growth in media with and without the detergent Tween 80. MAb 24c5 bound to Erdman and CDC 1551 at all culture times with only slightly greater apparent affinity after extended culture in the absence of Tween 80, indicating that a stable amount of GC polysaccharide antigen is associated with the cell surface of M. tuberculosis. An enzyme-linked immunosorbent assay indicated that GC is antigenically similar to glycogen, and the amount of GC antigen increased in the media of M. tuberculosis cultures grown either with or without the detergent Tween 80. Other nontuberculosis mycobacteria have antigenically similar GCs on their surfaces after in vitro growth. Inoculation of mice with live bacilli but not inoculation with dead bacilli elicited a strong antibody response to GC consistent with production of this antigen in vivo. Our results provide a more comprehensive picture of the M. tuberculosis cell envelope and the conditions that allow expression of M. tuberculosis GC.

scholarly journals Assessing the role of deoD gene in Mycobacterium tuberculosis in vitro growth and macrophage infection

2018 ◽  
Vol 119 ◽  
pp. 60-64 ◽  
Author(s):  
Pedro Ferrari Dalberto ◽  
Valnês Rodrigues-Junior ◽  
Virginia Carla Almeida Falcão ◽  
Antônio Frederico Michel Pinto ◽  
Bruno Lopes Abbadi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
In Vitro Growth ◽  
Macrophage Infection

scholarly journals In vitro growth inhibition and bactericidal activity of spathulenol against drug-resistant clinical isolates of Mycobacterium tuberculosis

2019 ◽  
Vol 29 (6) ◽  
pp. 798-800 ◽  
Author(s):  
Angel de Jesús Dzul-Beh ◽  
Karlina García-Sosa ◽  
Andrés Humberto Uc-Cachón ◽  
Jorge Bórquez ◽  
Luis A. Loyola ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Growth Inhibition ◽  
Bactericidal Activity ◽  
Clinical Isolates ◽  
In Vitro Growth ◽  
Drug Resistant

Inhibition of the in-vitro growth of Mycobacterium tuberculosis by a phytosiderophore

2001 ◽  
Vol 50 (10) ◽  
pp. 916-918 ◽  
Author(s):  
J. RAJIV ◽  
T. DAM ◽  
S. KUMAR ◽  
M. BOSE ◽  
K.K. AGGARWAL ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
In Vitro Growth

scholarly journals Deletion of the Mycobacterium tuberculosis pknH Gene Confers a Higher Bacillary Load during the Chronic Phase of Infection in BALB/c Mice

2005 ◽  
Vol 187 (16) ◽  
pp. 5751-5760 ◽  
Author(s):  
K. G. Papavinasasundaram ◽  
Bosco Chan ◽  
Ji-Hae Chung ◽  
M. Joseph Colston ◽  
Elaine O. Davis ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Parental Strain ◽  
Chronic Phase ◽  
In Vitro Growth ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Genes Encoding ◽  
Survival Studies ◽  
Mycobacterial Cell Wall

ABSTRACT The role of the serine/threonine kinase PknH in the physiology and virulence of Mycobacterium tuberculosis was assessed by the construction of a pknH deletion mutant. Deletion of the pknH gene did not affect sensitivity to the antimycobacterial drug ethambutol, although it was previously thought to be involved in regulating expression of emb genes encoding arabinosyl transferases, the targets of ethambutol. Nevertheless, transcription analyses revealed that genes associated with mycobacterial cell wall component synthesis, such as emb and ini operons, are downstream substrates of the PknH signaling cascade. In vitro survival studies revealed that a mutant with a deletion of the pknH gene displayed increased resistance to acidified nitrite stress, suggesting that nitric oxide is one of the potential environmental triggers for PknH activation. The effect of pknH deletion on mycobacterial virulence was investigated in BALB/c mice. In this model, the ΔpknH mutant was found to survive and replicate to a higher bacillary load in mouse organs than its parental strain and the pknH-complemented strain. In contrast, another closely related kinase mutant, the ΔpknE mutant, obtained from the same parental strain, was not affected in its virulence phenotype. Infection of THP-1 cells or in vitro growth studies in 7H9 medium did not reveal a significant in vitro growth advantage phenotype for the ΔpknH mutant. In conclusion, we propose that the serine/threonine kinase PknH plays a role in regulating bacillary load in mouse organs to facilitate adaptation to the host environment, possibly by enabling a regulated chronic infection by M. tuberculosis.

scholarly journals The six mammalian cell entry proteins (Mce3A‐F) encoded by the mce3 operon are expressed during in vitro growth of Mycobacterium tuberculosis

2006 ◽  
Vol 20 (5) ◽  
Author(s):  
Sherief El‐Shazly ◽  
Suhail Ahmad ◽  
Abu Salim Mustafa ◽  
Raja Al‐Attiyah
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mammalian Cell ◽  
Cell Entry ◽  
In Vitro Growth

scholarly journals Changes in the major cell envelope components of Mycobacterium tuberculosis during in vitro growth

Glycobiology ◽  
2013 ◽  
Vol 23 (8) ◽  
pp. 926-934 ◽  
Author(s):  
Lanhao Yang ◽  
Tejas Sinha ◽  
Tracy K Carlson ◽  
Tracy L Keiser ◽  
Jordi B Torrelles ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Envelope ◽  
In Vitro Growth

scholarly journals The Six Mammalian Cell Entry Proteins (Mce3A-F) Encoded by the mce3 Operon are Expressed During In Vitro Growth of Mycobacterium tuberculosis

2005 ◽  
Vol 62 (1) ◽  
pp. 16-24 ◽  
Author(s):  
S. Ahmad ◽  
S. El-Shazly ◽  
A. S. Mustafa ◽  
R. Al-Attiyah
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mammalian Cell ◽  
Cell Entry ◽  
In Vitro Growth

Effects of Liposomes of Different Lipid Composition on In Vitro Growth of Mycobacterium Tuberculosis H37Rv

2009 ◽  
Vol 148 (5) ◽  
pp. 797-799 ◽  
Author(s):  
G. M. Sorokoumova ◽  
S. M. Andreevskaya ◽  
T. G. Smirnova ◽  
E. E. Petrova ◽  
Yu. A. Zhogina ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lipid Composition ◽  
Tuberculosis H37rv ◽  
In Vitro Growth ◽  
Mycobacterium Tuberculosis H37rv

Tuberkulose - Screening

2011 ◽  
Vol 68 (7) ◽  
pp. 381-387
Author(s):  
Otto Schoch
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Interferon Gamma ◽  
Wird Eine ◽  
Interferon Gamma Release Assays

Das primäre Ziel der Aktivitäten zur bevölkerungsbezogenen Tuberkulosekontrolle ist die Identifizierung von Patienten mit sputummikroskopisch positiver Lungentuberkulose. Wenn diese Patienten umgehend therapiert werden, haben sie nicht nur eine optimale Heilungschance, sondern übertragen auch den Krankheitserreger nicht weiter auf andere Personen. Das Screening, die systematische Suche nach Tuberkulose, erfolgt in der Regel radiologisch bei der Suche nach Erkrankten, während immunologische Teste bei der Suche nach einer Infektion mit Mycobacterium tuberculosis zur Anwendung kommen. Diese Infektion, die ein erhöhtes Risiko für die Entwicklung einer Tuberkulose-Erkrankung mit sich bringt, wird im Rahmen der Umgebungsuntersuchungen oder bei Hochrisikogruppen gesucht. Neben dem traditionellen in vivo Mantoux Hauttest stehen heute die neueren in vitro Blutteste, die sogenannten Interferon Gamma Release Assays (IGRA) zur Verfügung, die unter anderem den Vorteil einer höheren Spezifität mit sich bringen, weil die verwendeten Antigene der Mykobakterien-Wand beim Impfstamm Bacille Calmitte Guerin (BCG) und bei den meisten atypischen Mykobakterien nicht vorhanden sind. Zudem kann bei Immunsupprimierten dank einer mitgeführten Positivkontrolle eine Aussage über die Wahrscheinlichkeit eines falsch negativen Testresultates gemacht werden. Bei neu diagnostizierter Infektion mit Mycobacterium tuberculosis wird eine präventive Chemotherapie mit Isoniazid während 9 Monaten durchgeführt.

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