scholarly journals FUNGOS ASSOCIADOS À CASCA DO CAULE DE Platanus orientalis L.

2004 ◽  
Vol 26 (54) ◽  
Author(s):  
Denise Zelak Leite Bastos ◽  
Ida Chapaval Pimentel ◽  
Christian Dykstra ◽  
Carlos Eduardo Kania ◽  
Juarez Gabardo ◽  
...  
Keyword(s):  
Sabouraud Dextrose Agar ◽  
Trichoderma Sp ◽  
Platanus Orientalis ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Para posterior emprego na biotransformação do ácido betulínico e ácido betulônico, foram isolados fungos epifíticos e endofíticos da casca de caule de Platanus orientalis L. de acordo com metodologia específica. Para cada experimento foram utilizadas 800 amostras cortadas em pequenos pedaços e plaqueadas em dois meios de culturas diferentes: BDA (batata-dextrose-ágar) e SDA (sabouraud-dextrose-ágar). Os dados foram expressos em incidência de aparecimento do fungo. Foi realizado teste qui-quadrado (÷˝) para verificar se algum dos meios favoreceu o isolamento e cultivo dos fungos isolados. Foram identificados 13 gêneros de fungos epifíticos: Mucor sp.; Rhizopus sp.; Dematium sp.; Chaetophoma sp.; Arthobotrys sp.; Trichoderma sp.; Drecheslera sp.; Nigrospora sp.; Fusarium sp.; Pestalotia sp.; Rhizomucor sp.; Aspergillus sp. e Alternaria sp. Constatou-se que Mucor sp., Rhizopus sp. apresentaram as maiores incidências nos dois meios. Oito gêneros foram identificados para os fungos endofíticos: Mucor sp.; Stachibotrys sp.; Trichophyton sp.; Trichoderma sp.; Aspergillus sp.; Phoma sp.; Fusarium sp. e Curvularia sp. Observaram-se as maiores incidências dos fungos: Mucor sp. presente nos dois meios; Stachibotrys sp. em BDA; Trichophyton sp. em SDA. Pôde-se comprovar pelo teste (÷˝) que para alguns fungos a alteração do meiofoi significativa.

scholarly journals Associação de Fungos com Sementes de Espécies Florestais Nativas

2017 ◽  
Vol 43 (3) ◽  
pp. 246-247 ◽  
Author(s):  
Ana Lídia Moura do Carmo ◽  
Edson José Mazaratto ◽  
Bárbara Eckstein ◽  
Álvaro Figueredo dos Santos
Keyword(s):  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

RESUMO No Brasil, ainda há poucas pesquisas sobre os microrganismos associados às sementes florestais. O objetivo desse trabalho foi detectar fungos associados às sementes de seis espécies florestais nativas: açoita-cavalo (Luehea divaricata), araçá amarelo (Psidium cattleyanum), corticeira (Erythrina falcata), rabo-de-bugio (Lonchocarpus sp.), vassoura-vermelha (Dodonea viscosa) e pau-jacaré (Piptadenia gonoacantha). Foram identificados oito gêneros de fungos potencialmente patogênicos: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. e Botrytis sp. e fungos saprófitas: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. e Trichothecium sp. Os resultados evidenciam a diversidade de fungos associados às sementes florestais nativas, incluindo vários potencialmente fitopatogênicos.

scholarly journals Trigona sp. COMO VISITANTE FLORAL E VETOR DE ESPOROS FÚNGICOS PARA GOIABEIRA (Psidium guajava L. – MYRTACEAE)

2004 ◽  
Vol 26 (55) ◽  
Author(s):  
Rodrigo Makowiecky Stuart ◽  
Carolina Lamas ◽  
Ida Chapaval Pimentel
Keyword(s):  
Psidium Guajava ◽  
Penicillium Sp ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Mycelia Sterilia

Este trabalho foi realizado para avaliar a ação de Trigona sp. como visitante floral e vetor de esporos fúngicos para goiabeira (Psidium guajava L.). As observações foram feitas entre dezembro de 2003 e fevereiro de 2004 em flores de goiabeiras do Setor de Ciências Biológicas, Universidade Federal do Paraná. Foi verificado que três gêneros de abelhas estavam visitando as flores de goiaba: Apis melifera, Bombus sp. e Trigona sp.. Entretanto, Trigona demonstrou ser mais freqüente que as outras durante as observações. A freqüência de visitas de Trigona foi maior durante o início da manhã, decrescendo ao longo do dia. A avaliação dos fungos associados a Trigona demonstrou a presença de 11 gêneros distintos: Acremonium sp., Altenaria sp., Aspergillus sp., Colletotrichum sp., Curvularia sp., Fonsecaea sp., Fusarium sp., Mycelia sterilia, Penicillium sp., Phoma sp. e Rhizopus sp.. 48 % destes representam fitopatógenos potenciais como Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. e Phoma sp.. Estes dados demonstram que o gênero Trigona pode atuar na disseminação de doenças para diversas culturas, funcionando como vetor de esporos fúngicos para outras plantas.

INVENTARISASI JAMUR PADA BENIH BEBERAPA VARIETAS KEDELAI [Glycine max (L.) Merril] YANG BERADA DI SEKITAR BANDAR LAMPUNG

2014 ◽  
Vol 2 (3) ◽  
Author(s):  
Reny Destya Angrum ◽  
Muhammad Nurdin ◽  
Suskandini Ratih ◽  
Hasriadi Mat Akin
Keyword(s):  
Glycine Max ◽  
Agar Plate ◽  
Plate Test ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Glycine Max L ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Penelitian ini bertujuan untuk menginventarisasi jamur yang terdapat pada beberapa varietas benih kedelai [ Glycine max (L.) Merril ] yang berada di sekitar Bandar Lampung. Penelitian ini dilakukan di Laboratorium Penyakit Tanaman, UniversitasLampung dari Mei 2013 sampai dengan Agustus 2013. Penelitian ini menggunakan kedelai varieta Burangrang, Kaba, Argomulyo, Tanggamus dan Grobogan. Benih-benih tersebut diinkubasi menggunakan metode blotter test dan agar plate test dengan dua perlakuan, yaitu kontrol (tanpa NaOCl) dan dengan menggunakan NaOCl. Jamur-jamur yang tumbuh dari benih yang diinkubasi diidentifikasi dengan cara diamati dan difoto, selanjutnya dicocokkan dengan bubu-buku referensi tentang penyakit- penyakit kedelai. Selain itu juga dilakukan penghitungan persentase kemunculan jamur dari benih yang diinkubasi. Hasil yang diperoleh dari penelitian yang dilakukan dengan perlakuan desinfeksi maupun tanpa desinfeksi ditemukan jenis jamur yang berasal dari lapang (Fusarium sp., Cercospora sp., Sclerotium sp., Phomopsis sp., Rhizoctonia sp., dan Colletotrichum sp.) dan jenis jamur yang berasal dari gudang penyimpanan (Trichoderma sp., Aspergillus sp., Penicillium sp., dan Rhizopus sp.). Benih yang diberikan perlakuan desinfeksi dapat mengurangi tumbuhnya jamur yang berasal dari gudang/tempat penyimpanan (kontaminan) tetapi tidak menghilangkan jenis jamur yang menginfeksi di dalam benih (jamur yang berasal dari lapang). Pada varietas Grobogan Rhizoctonia sp. memiliki persentase kemunculan jamur yang lebih tinggi dibandingkan dengan jamur lainnya yaitu sebesar 60,56%. Pada benih dengan perlakuan tanpa desinfeksi persentase kemunculan Aspergillus sp. pada varietas Kaba dan Tanggamus lebih tinggi dibandingkan dengan jamur lainnya sebesar 63,33%. Pada media perlakuan desinfeksi dengan menggunakan media kertas saring persentase kemunculan jamur pada varietas Kaba Cercospora sp. memiliki persentase lebih tinggi dibandingkan dengan jamur lainnya sebesar 30%. Pada media perlakuan tanpa desinfeksidengan menggunakan media kertas saring, Trichoderma sp. pada varietas Kaba memiliki persentase kemunculan jamur lebih tinggi dibandingkan dengan jamur lainnya, dengan persentase kemunculan masing-masing sebesar 77,23%.

scholarly journals Occurrence and Diversity of Soil Microflora in Potato Fields of Bangladesh

2020 ◽  
pp. 1-15
Author(s):  
M. A. Nitu ◽  
M. Rahaman ◽  
F. M. Aminuzzaman ◽  
N. Sultana
Keyword(s):  
Rhizosphere Soil ◽  
Fungal Species ◽  
Soil Microflora ◽  
Bacterium Species ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Soil Dilution ◽  
Made In

Microflora from potato rhizosphere soil was isolated from different potato fields of Bangladesh. Seventeen soil samples were analyzed for the presence of microflora in selected potato field soils. Seven fungal species and one bacterium species were morphologically characterized using soil dilution and streak plate methods. The predominant fungi isolated including Alternaria sp., Aspergillus sp., Penicillium sp., Rhizopus sp., Bipolaris sp., Phytophthora sp., Fusarium sp. and one bacterium was identified as Ralstonia solanacearum. Individual colonies of fungi and bacteria were counted on Potato Dextrose Agar (PDA), V8 juice Agar and their presence in soil was compared in respect of different locations of potato fields. The occurrence of Phytophthora sp. was medium in Tongibari and lower in Singair Union, Sonargaon, Matlab Dakshin, Gobindaganj, Palashbari, Gopinathpur and Bagmara. The highest counts of R. solanacearum were found in Singair Union, Tongibari and Daudkandi and the lowest counts were made in Palashbari and Bagmara. This was the first reported examination of the microbial diversity of soil microflora in some selected potato fields of Bangladesh.

scholarly journals Germinação, qualidade sanitária e armazenamento de sementes de canafístula (Peltophorum dubium)

2012 ◽  
Vol 36 (1) ◽  
pp. 01-06 ◽  
Author(s):  
Adriana Martinelli Seneme ◽  
Edilberto Possamai ◽  
Silvelena Vanzolini ◽  
Cibele Chalita Martins
Keyword(s):  
Fusarium Semitectum ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

Neste trabalho, objetivou-se avaliar o efeito de métodos de superação de dormência e do ambiente de armazenamento sobre a qualidade fisiológica e fitopatológica das sementes de canafístula (Peltophorum dubium). As sementes foram submetidas aos seguintes tratamentos de superação de dormência: escarificação com lixa (200); imersão em água na temperatura ambiente, durante 24 e 72 h; imersão em ácido sulfúrico por 2, 6, 10, 15, 20 e 30 min; imersão em água quente (70, 80 e 90 C); e umedecimento do substrato com solução de KNO3 (0,2%). As sementes foram armazenadas na temperatura ambiente e a 10 C por 210 dias. Os efeitos dos tratamentos e do armazenamento foram avaliados por meio do teor de água, teste de germinação (cinco repetições de 30 sementes), de comprimento de plântulas e sanidade (400 sementes), com incubação por oito dias (22-25 C). Na análise estatística dos dados, utilizou-se o delineamento experimental inteiramente casualizado em esquema fatorial 2 x 14 (condições de armazenamento x tratamentos para a superação da dormência). As médias foram comparadas pelo teste de Tukey (P>0,5). Com relação às sementes não armazenadas, os melhores tratamentos para superar a dormência e promover a germinação foram escarificação com lixa ou ácido sulfúrico por 15 a 30 min; quanto às sementes armazenadas, houve a imersão em água quente (70 a 80 ºC). Os fungos detectados nas sementes foram Pestalotia sp., Alternaria sp., Rhizopus sp., Nigrospora sp., Curvularia sp., Fusarium sp., Rhizoctonia sp., Aspergillus sp., Cladosporium sp. e Fusarium semitectum.

scholarly journals Isolamento e identificação da microbiota fúngica e de dermatófitos da pele de eqüinos hígidos e daqueles afetados por dermatofitose

1996 ◽  
Vol 33 (3) ◽  
pp. 170 ◽  
Author(s):  
Marcia Mayumi Ishikawa ◽  
Ronaldo Lucas ◽  
Carlos Eduardo Larsson ◽  
Walderez Gambale ◽  
Wilson Roberto Fernandes
Keyword(s):  
Microsporum Canis ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Scopulariopsis Brevicaulis ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aureobasidium Sp ◽  
Aspergillus Sp

Pela inexistência na literatura latino-americana e brasileira de trabalhos que enfoquem a microbiota fúngica de eqüinos hígidos e as principais espécies de dermatófitos em casos de eqüinos com lesões sugestivas de infecção dermatofítica do tegumento cutâneo, utilizaram-se 175 eqüinos, de ambos os sexos, tanto de raça definida como daqueles sem perfeita definição racial, de diferentes idades e que foram reunidos em 2 grupos. O GRUPO I composto de 133 eqüinos assintomáticos e desprovidos de lesões cutâneas, dos quais, após exame dermatológico, interposiçãoda Luz de Wood (48 eqüinos), colheram-se, pela técnica do carpete, material que foi semeado em meios de ágar Sabouraud Dextrose, Mycobiotic ágar, Tricophyton ágar 3, Tricophyton ágar 5 e incubados a 25° e 37°C durante 30 dias. Isolaram-se: Penicillium sp (80,4%), fíhizopus sp (62,4%), Aspergillus sp (41,3%), Fusarium sp (40,6%), Cladosporium sp (33,1%), Trichoderma sp (21,0%), Mucorsp (18,0%), Epicoccum sp (12,0%), Mycelia sterillia (8,8%), Rhodotorula sp (7,5%), Neurospora sp (4,5%), Alternaria sp (3,7%), Aureobasidium sp (3,7%), Geotrichum sp (3,0%), Paecilomyces sp (2,2%), Monascus sp (2,2%), Cephalosporium sp (1,5%), Nigrospora sp (0,7%), Scopulariopsis brevicaulis (0,7%), Trichosporon sp (0,7%). O GRUPO II foi composto por 42 eqüinos portadores de lesões sugestivas de dermatofitose que, após terem sido submetidos a exame dermatológico, expostos à luz de Wood (22 eqüinos), tiveram pelame e crostas submetidos a cultivo micológico, isolando-se em 6 (14,3%) cepas de Dermatophylus congolensis eem 3 (7,1%) eqüinos houve o crescimento de dermatófitos da espécie Microsporum canis. Dos 70 eqüinos expostos à radiação ultravioleta observou-se falsa fluorescência em 2 animais.

Yeasts and Molds Isolated from Spoiling Citrus Products and By-Products1

1989 ◽  
Vol 52 (4) ◽  
pp. 261-263 ◽  
Author(s):  
MICKEY E. PARISH ◽  
DIANNE P. HIGGINS
Keyword(s):  
Orange Juice ◽  
Aureobasidium Pullulans ◽  
Candida Maltosa ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Pichia Membranaefaciens ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Yeasts And Molds

A variety of citrus products, including dried peel used as livestock fodder, unpasteurized orange juice and pasteurized, and chilled orange juice were selectively surveyed for their fungal microflora. Fungi isolated were 1) pasteurized orange juice: Aureobasidium pullulans, Cladosporium sp., and Penicillium sp., 2) unpasteurized orange juice: Candida maltosa, C. sake, Fusarium sp., Geotrichum sp., Hanseniaspora sp., H. guilliermondii, Penicillium sp., Pichia membranaefaciens, Saccharomyces cerevisiae, Schwanniomyces occidentalis, and Torulaspora delbrueckii and 3) dried peel: Aspergillus niger, Aspergillus sp., Byssochlamys sp., Fusarium sp., Penicillium sp., Rhizopus sp., Rhodotorula sp., Sc. occidentalis, and Trichoderma sp.

scholarly journals Health and transmission of fungi associated with seeds of Luehea divaricata

2020 ◽  
Vol 14 ◽  
Author(s):  
Alexsandra Cezimbra Quevedo ◽  
Marlove Fatima Brião Muniz ◽  
Clair Walker ◽  
Mateus Alves Saldanha
Keyword(s):  
Native Forest ◽  
Germination Test ◽  
Seedling Roots ◽  
Physiological Quality ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Health Test ◽  
Transmission Test

Research related to the pathology of seeds of native forest species, açoita-cavalo (Luehea divaricata), is scarce. This study aimed to evaluate the sanitary and physiological quality of whipshorse seeds and the possible transmission of fungi associated with the seeds to the seedlings, in lots from Santa Maria - RS. Were 100 seeds used, divided into four repetitions of 25 per lot (2016 and 2018) and test. The health test was carried out in which the seeds without asepsis were placed in “gerbox” boxes with filter paper substrate at a temperature of 25 °C, with a photoperiod of 12 h for seven days. The germination test and the fungi transmission test seeds were conducted in a germination room at a temperature of 25 ºC, and a photoperiod of 12 h. Only the seeds used in the germination test underwent asepsis. The evaluations of the two tests consisted of the First Germination Count (at 14 days) and the count in the number of normal, abnormal seedlings, and non-germinated seeds (at 28 days). In the health test, fungi were identified and quantified: Fusarium sp., Alternaria sp., Aspergillus sp., Penicillium sp., Rhizopus sp., Cladosporium sp., Pestalotia sp. and Epicoccum sp. The Fusarium genus was identified associated with the symptoms of browning and rotting in seedling roots in the transmission test. The sample from 2018 showed greater vigor, reaching 21% at 14 days, not different from the lot of 2016 that reached 10%. The lot of 2018 presented the best results concerning health, vigor, and germination.

scholarly journals Isolation frequency of medically important fungi and fluconazole resistant of Candida sp from hospital cockroaches.

2020 ◽  
Author(s):  
Doreen Anna Mloka ◽  
George M Bwire ◽  
Kenndy Mwambete
Keyword(s):  
Fungal Pathogens ◽  
Isolation And Identification ◽  
Opportunistic Fungi ◽  
Candida Sp ◽  
Microdilution Method ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Parasitic Pathogens

Abstract BackgroundCockroaches are common pests in homes and hospitals. They are known to cause allergic reactions in certain individuals and have found to be potential vectors for various bacterial and parasitic pathogens. This study assessed the potential of hospital cockroaches to act as vectors of medically important fungal pathogens on their external surfaces.MethodsCockroaches were captured from the main Intensive care unit (ICU), burn unit, adult surgical wards , pediatric oncology wards, intern hostel kitchen and the central kitchen of a national referral teaching hospital in Tanzania. Normal saline washings from the external surface of cockroaches were cultured on standard mycological media to facilitate isolation and identification of medically important molds and yeasts. Susceptibility of Candida sp isolates to fluconazole was tested using the CLSI M27-A3 microdilution method.ResultsAt total of 72 cockroaches were captured from various sites of the hospital between February and March 2015. All cockroaches captured were shown to carry medically importance fungi. A total of 956 medically important fungi were isolated, 57.9 % were Candida sp., 23.2%, Aspergillus sp. , 3.1% Cladosporium sp., 1.8% Rhizopus sp., 1.2 % Geotrichum sp., 0.9 % Pencillum sp. , 0.7% Alternaria sp, 0.6 % Fusarium sp , 0.3% Mucor sp and 10.1% others. Aspergillus fumigatus (50.0%) was the most commonly isolated followed by Aspergillus niger ( 15.8%) among the Aspergillus isolates.Over 16.3 % of the Candida isolates not intrinsically resistant to fluconazole showed resistance to this drug.mResistance was most frequently found in Candidapseudotropicalis (23.8%) and Candida glabarata (20.0%) and least in isolates of Candida albicans ( 6.3%). ConclusionThe external surfaces of cockroaches from this hospital may act as reservoirs of medically important opportunistic fungi exhibiting resistance to fluconazole.

scholarly journals Production of Exopolysaccharide from Local Fungal Isolate

2017 ◽  
Vol 5 (3) ◽  
pp. 338-346 ◽  
Author(s):  
Alaa Jabbar Al-Manhel
Keyword(s):  
Culture Media ◽  
Exopolysaccharide Production ◽  
Maximum Production ◽  
Penicillium Sp ◽  
Alternaria Sp ◽  
Substitution Ratio ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Layer Chromatography

The study investigated in isolation of 26 fungal isolates belonging to 6 different genera viz., Penicillium sp., Aspergillus sp. , Fusarium sp., Alternaria sp., , Rhizopus sp. and Phoma sp. Were screened for exopolysaccharide production. Glucose in culture media was studied to select the medium that gives a maximum production of exopolysaccharide by Penicillium sp., Exopolysaccharide was isolated by ethanol precipitation. The medium which contains glucose had been selected to get the heights production of exopolysaccharide . Fermentation conditions were further investigated to optimize exopolysaccharide production by Penicillium sp. , The optimum substitution ratio, temperatures, pH and incubation periods for the maximum production of the polysaccharide were 100% , 30 ˚C , pH 5 and 9 days respectively. Characteristic of exopolysaccharide compounds were observed in the FTIR spectrum. Thin layer chromatography of the hydrolyzed polysaccharide showed that the exopolysaccharide production was heteropolysaccharide consists of galactose, glucose and mannose.

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