scholarly journals Peer Review #2 of "Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures (v0.1)"

2016 ◽  
Author(s):  
W Cawthorn
Keyword(s):  
Adipose Tissue ◽  
Quantitative Analysis ◽  
Peer Review ◽  
Cell Volume ◽  
Cell Cultures ◽  
Tissue Cell ◽  
Cell Recovery ◽  
Fat Cell ◽  
Adipose Tissue Cell ◽  
Rat Adipose Tissue

scholarly journals Dietary Conjugated Linoleic Acid Reduces Rat Adipose Tissue Cell Size Rather than Cell Number

2000 ◽  
Vol 130 (6) ◽  
pp. 1548-1554 ◽  
Author(s):  
Michael J. Azain ◽  
Dorothy B. Hausman ◽  
Matthew B. Sisk ◽  
William P. Flatt ◽  
Dennis E. Jewell
Keyword(s):  
Adipose Tissue ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Cell Size ◽  
Cell Number ◽  
Tissue Cell ◽  
Adipose Tissue Cell ◽  
Dietary Conjugated Linoleic Acid ◽  
Rat Adipose Tissue

scholarly journals ПОРІВНЯЛЬНА ХАРАКТЕРИСТИКА ФЕНОТИПОВИХ ЗМІН КУЛЬТУР КЛІТИН ЖИРОВОЇ ТКАНИНИ ТА КІСТКОВОГО МОЗКУ В ПРОЦЕСІ КУЛЬТИВУВАННЯ

2017 ◽  
pp. 113-119 ◽  
Author(s):  
В. В. Ковпак ◽  
О. С. Ковпак
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Cell Cultures ◽  
Bone Marrow Cells ◽  
Primary Cultures ◽  
Tissue Cell ◽  
Adipose Tissue Cell ◽  
Polygonal Shape ◽  
Adipose Tissue Cells ◽  
Number Of Cells

У статті описані дані щодо зміни фенотипу культур клітин жирової тканини (ККЖТ) та кісткового мозку (КККМ) у процесі культивування. Дослідження первинних культур клітин кісткового мозку та жирової тканини щура показали, що вони морфологічно гетерогенні, у їх склад входили: невелика кількість клітин полігональної форми, а основну масу складали фібробластоподібні. За подальшого культивування відмічали процес переходу від гетерогенних культур на нульовому  пасажі до найбільш гомогенних у кінці дослідження. Нами були відмічені відмінності у імунофенотипі культур клітин кісткового мозку та жирової тканини, які не зникали з пасажами. This article describes the changes in phenotype of cultures of adipose tissue cells (ATCC) and bone marrow cells (BMCC) in the process of cultivation. Study of primary cultures of cells of the bone marrow and adipose tissue of rat has shown that they are morphologically heterogeneous, they included: a small number of cells of polygonal shape, and the bulk was fìbroblast-like cells. Process of transition from the heterogeneous cultures at zero passaging to the most homogeneous at the end of the study was noted during further cultivation. We noted differences in immunophenotype of bone marrow and adipose tissue cell cultures that did not disappear with passaging.

Adipose tissue metabolism and cell size: variation between subcutaneous sites and the effect of copper supplementation

1987 ◽  
Vol 45 (1) ◽  
pp. 75-80 ◽  
Author(s):  
P. A. Sinnett-Smith ◽  
J. A. Woolliams
Keyword(s):  
Adipose Tissue ◽  
Cell Volume ◽  
Lipolytic Activity ◽  
Subcutaneous Fat ◽  
Size Variation ◽  
Tissue Cell ◽  
Fat Depots ◽  
Adipose Tissue Cell ◽  
Proportional Increase ◽  
Effect Of Copper

AbstractAdipose tissue cell volume, lipolytic rates, Iipoprotein lipase (LPL) and acetyl CoA carboxylase (ACC) activities were determined in biopsy samples taken from the back, shoulder, rump and groin subcutaneous fat depots of 17-month-old female sheep (no. = 24). Half of the sheep had received a copper (Cu) supplement at 6 weeks of age. Biopsy samples were taken after fasting overnight.Differences in cell volume and ACC activity were apparent between sites. Supplementation with Cu at 6 weeks of age led to a proportional increase of between 0·24 and 0·45 in adipose cell volume and a proportional increase in lipolysis which varied from 0·70 to 2·27 depending on site and hormone addition. Correlations between sites for cell volume and lipolytic activity were not high in general but those for ACC and LPL activities were higher (for ACC r = 0·40 to 0·79). Overall the best correlations were between the back and rump sites. Correlations between the various metabolic measurements and cell volume were not apparent within treatment groups.

scholarly journals Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures

2016 ◽  
Author(s):  
Floriana Rotondo ◽  
María del Mar Romero ◽  
Ana Cecilia Ho-Palma ◽  
Xavier Remesar ◽  
José Antonio Fernández-López ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Content ◽  
Primary Cultures ◽  
Cell Mass ◽  
Lactate Production ◽  
Tissue Cell ◽  
Cell Recovery ◽  
Fat Cell ◽  
Isolated Cells ◽  
Adult Rats

Background. White adipose tissue (WAT) is a complex, disperse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT breaking up the matrix collagen fibres, but primary cultures of these cells could not be easily correlated to intact WAT, since often recovery and viability are unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates into an extremely high (with respect to the actual "live cytoplasm" size) metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

scholarly journals Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2725 ◽  
Author(s):  
Floriana Rotondo ◽  
María del Mar Romero ◽  
Ana Cecilia Ho-Palma ◽  
Xavier Remesar ◽  
José Antonio Fernández-López ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Metabolic Activity ◽  
Lipid Content ◽  
Primary Cultures ◽  
Cell Mass ◽  
Tissue Cell ◽  
Cell Recovery ◽  
Fat Cell ◽  
Isolated Cells ◽  
Adult Rats

BackgroundWhite adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defense, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres; however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown.Experimental DesignEpididymal WAT of four young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of glucose uptake and lactate, glycerol and NEFA excretion rates up to 48 h. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were measured. The presence of non-nucleated cells (erythrocytes) was also estimated.ResultsCell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70–75%. Cells showed even higher metabolic activity in the second than in the first day of incubation. Adipocytes were 7%, erythrocytes 66% and other stromal (nucleated cells) 27% of total WAT cells. However, their overall volumes were 90%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 1.3% of WAT.ConclusionsThe methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have also found that the “live cell mass” of adipose tissue is very small: about 13 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood (the rats were killed by exsanguination). These data translate (with respect to the actual “live cytoplasm” size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

scholarly journals Quantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures

2016 ◽  
Author(s):  
Floriana Rotondo ◽  
María del Mar Romero ◽  
Ana Cecilia Ho-Palma ◽  
Xavier Remesar ◽  
José Antonio Fernández-López ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Content ◽  
Primary Cultures ◽  
Cell Mass ◽  
Lactate Production ◽  
Tissue Cell ◽  
Cell Recovery ◽  
Fat Cell ◽  
Isolated Cells ◽  
Adult Rats

Background. White adipose tissue (WAT) is a complex, disperse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT breaking up the matrix collagen fibres, but primary cultures of these cells could not be easily correlated to intact WAT, since often recovery and viability are unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates into an extremely high (with respect to the actual "live cytoplasm" size) metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

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