scholarly journals Peer Review #1 of "Characterization of carbonate fraction of the Atlantic bluefin tuna fin spine bone matrix for stable isotope analysis (v0.1)"

2019 ◽  
Author(s):  
F Coloma
Keyword(s):  
Stable Isotope ◽  
Peer Review ◽  
Stable Isotope Analysis ◽  
Bone Matrix ◽  
Isotope Analysis ◽  
Bluefin Tuna ◽  
Atlantic Bluefin Tuna

scholarly journals Protocol for Sampling Sequential Fin Spine Growth Intervals for Isotope Analysis in the Atlantic Bluefin Tuna

2020 ◽  
Vol 7 ◽  
Author(s):  
Patricia Lastra Luque ◽  
Saburo Sakai ◽  
Hilario Murua ◽  
Haritz Arrizabalaga
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Sequential Sampling ◽  
Isotope Analysis ◽  
Bluefin Tuna ◽  
Annual Growth ◽  
Micro Milling ◽  
Carbonate Content ◽  
Growth Band ◽  
Atlantic Bluefin Tuna

BackgroundMicromilled fish otoliths (ear bones) have been widely used for stable oxygen (δ18O) and carbon (δ13C) isotope analysis. The first dorsal fin spine is routinely used for ageing in the eastern Atlantic bluefin tuna (ABFT) population; however, stable isotope analysis remains unapplied in this hard structure. The objective of the present protocol is to achieve a sequential sampling of growth layers in the ABFT fin spine at high spatial resolution and along growth trajectory for stable isotope analysis.MethodsWe used a micromilling and micro-powder collecting technique for sequentially sampling annual growth layers of the fin spine bone collected from freshly caught ABFT. We assessed the carbonate content and the optimal drilling amount to ensure enough powder quantity was recovered from each annual growth band to accommodate accurate measurement of the δ18O and δ13C values.ResultsThe optimal drilling path included 20 drilling lines in 49 μm, with 400 μm depth and 900 μm-line width, which represents a time resolution of about 2.5 months. The minimum powder quantity required from each annual growth layer for δ18O and δ13C isotope analysis was approximately 180 μg because the carbonate contents in the fin spine powder was 5–6%. The δ18O profile assayed in the last growth intervals coincide with the surface δ18Oseawater for the southern west waters off the Norwegian coast, where the tuna was caught. The fluctuating pattern in the second growth interval may reflect certain fidelity to the Norwegian SE and Swedish SW coast and/or otherwise to the US west coast. The δ13Cspine displayed a larger temporal variability along the growth transect that may be explained by several factors including differences in habitat use, behavior, and even trophic level.ConclusionBased on the result obtained, we present an optimal standard protocol for the sampling of sequential, annually formed growth layers in the fin spine bone for stable isotope analysis using the micro-milling as a high precision technique. This protocol is particularly useful in endangered and/or protected species for which fin spines represent a non-lethal alternative to otoliths opening new research avenues to improve their management and conservation.

Evidence of westward transoceanic migration of Pacific bluefin tuna in the Sea of Japan based on stable isotope analysis

2017 ◽  
Vol 164 (4) ◽  
Author(s):  
Atsushi Tawa ◽  
Taiki Ishihara ◽  
Yuki Uematsu ◽  
Tsuneo Ono ◽  
Seiji Ohshimo
Keyword(s):  
Stable Isotope ◽  
Sea Of Japan ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Bluefin Tuna ◽  
The Sea Of Japan ◽  
Pacific Bluefin Tuna
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