scholarly journals Duckweed (Lemna minor) is a novel natural inducer of cellulase production in Trichoderma reesei

2018 ◽  
Author(s):  
Chen Li ◽  
Jun Feng ◽  
Xue Bai ◽  
Shulin Chen ◽  
Dongyuan Zhang ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Fermentation Process ◽  
Lemna Minor ◽  
Biomass Accumulation ◽  
Cellulase Production ◽  
Enzyme Hydrolysis ◽  
Hydrolysis Rate ◽  
Shake Flasks ◽  
The Cost ◽  
Rut C30

An inducer is crucial for cellulase production. In this study, duckweed was used as an inducer of cellulase production by Trichoderma reesei RUT C30. In a reaction induced by 50 g l-1 duckweed in shake flasks, the filter-paper activity (FPA) reached 6.5 FPU ml-1, a value comparable to that induced by avicel. The enzyme-hydrolysis rate induced by steam-exploded corn stalks was 54.2%, representing a 28% improvement over that induced by avicel. The duckweed starch was hydrolyzed to glucose, which was subsequently used for biomass accumulation during the fermentation process. Furthermore, to optimize control of the fermentation process, a combined substrate of avicel and duckweed was used to induce cellulase production by T. reesei RUT C30. The cellulase production and hydrolysis rates for the combined substrate, compared with avicel alone, were 39.6% and 36.7% higher, respectively. The results of this study suggest that duckweed is a good inducer of cellulase production in T. reesei, and it might aid in decreasing the cost of lignocellulosic-material hydrolysis.

scholarly journals Duckweed (Lemna minor) is a novel natural inducer of cellulase production in Trichoderma reesei

2018 ◽  
Author(s):  
Chen Li ◽  
Jun Feng ◽  
Xue Bai ◽  
Shulin Chen ◽  
Dongyuan Zhang ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Fermentation Process ◽  
Lemna Minor ◽  
Biomass Accumulation ◽  
Cellulase Production ◽  
Enzyme Hydrolysis ◽  
Hydrolysis Rate ◽  
Shake Flasks ◽  
The Cost ◽  
Rut C30

An inducer is crucial for cellulase production. In this study, duckweed was used as an inducer of cellulase production by Trichoderma reesei RUT C30. In a reaction induced by 50 g l-1 duckweed in shake flasks, the filter-paper activity (FPA) reached 6.5 FPU ml-1, a value comparable to that induced by avicel. The enzyme-hydrolysis rate induced by steam-exploded corn stalks was 54.2%, representing a 28% improvement over that induced by avicel. The duckweed starch was hydrolyzed to glucose, which was subsequently used for biomass accumulation during the fermentation process. Furthermore, to optimize control of the fermentation process, a combined substrate of avicel and duckweed was used to induce cellulase production by T. reesei RUT C30. The cellulase production and hydrolysis rates for the combined substrate, compared with avicel alone, were 39.6% and 36.7% higher, respectively. The results of this study suggest that duckweed is a good inducer of cellulase production in T. reesei, and it might aid in decreasing the cost of lignocellulosic-material hydrolysis.

Dynamics of Cellulase Production by Glucose Grown Cultures of Trichoderma reesei Rut-C30 as a Response to Addition of Cellulose

2004 ◽  
Vol 113 (1-3) ◽  
pp. 115-124 ◽  
Author(s):  
Nóra Szijártó ◽  
Zsolt Szengyel ◽  
Gunnar Lidén ◽  
Kati Réczey
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Rut C30

Kinetic model for batch cellulase production by Trichoderma reesei RUT C30

1997 ◽  
Vol 54 (2) ◽  
pp. 83-94 ◽  
Author(s):  
Svetlana Velkovska ◽  
Mark R Marten ◽  
David F Ollis
Keyword(s):  
Kinetic Model ◽  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Trichoderma Reesei Rut C30 ◽  
Rut C30

scholarly journals Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production in Trichoderma reesei

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Fangzhong Wang ◽  
Ruiqin Zhang ◽  
Lijuan Han ◽  
Wei Guo ◽  
Zhiqiang Du ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Trichoderma Reesei ◽  
Parent Strain ◽  
Fungal Growth ◽  
Biomass Accumulation ◽  
Hyphal Growth ◽  
Fold Increase ◽  
Cellulase Production ◽  
Fungal Species ◽  
New Strategy

Abstract Background Trichoderma reesei is widely used for cellulase production and accepted as an example for cellulase research. Cre1-mediated carbon catabolite repression (CCR) can significantly inhibit the transcription of cellulase genes during cellulase fermentation in T. reesei. Early efforts have been undertaken to modify Cre1 for the release of CCR; however, this approach leads to arrested hyphal growth and decreased biomass accumulation, which negatively affects cellulase production. Results In this study, novel fusion transcription factors (fTFs) were designed to release or attenuate CCR inhibition in cellulase transcription, while Cre1 was left intact to maintain normal hyphal growth. Four designed fTFs were introduced into the T. reesei genome, which generated several transformants, named Kuace3, Kuclr2, Kuace2, and Kuxyr1. No obvious differences in growth were observed between the parent and transformant strains. However, the transcription levels of cel7a, a major cellulase gene, were significantly elevated in all the transformants, particularly in Kuace2 and Kuxyr1, when grown on lactose as a carbon source. This suggested that CCR inhibition was released or attenuated in the transformant strains. The growth of Kuace2 and Kuxyr1 was approximately equivalent to that of the parent strain in fed-batch fermentation process. However, we observed a 3.2- and 2.1-fold increase in the pNPCase titers of the Kuace2 and Kuxyr1 strains, respectively, compared with that of the parent strain. Moreover, we observed a 6.1- and 3.9-fold increase in the pNPCase titers of the Kuace2 and Kuxyr1 strains, respectively, compared with that of Δcre1 strain. Conclusions A new strategy based on fTFs was successfully established in T. reesei to improve cellulase titers without impairing fungal growth. This study will be valuable for lignocellulosic biorefining and for guiding the development of engineering strategies for producing other important biochemical compounds in fungal species.

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