scholarly journals Genetic diversity and population structure in an invasive pantropical earthworm along an altitudinal gradient

2017 ◽  
Author(s):  
Diana Ortíz-Gamino ◽  
Luis Cunha ◽  
Esperanza Martínez-Romero ◽  
Norma Flores-Estévez ◽  
Ángel I Ortíz-Ceballos
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Altitudinal Gradient ◽  
Issr Markers ◽  
Invasion Success ◽  
Nuclear Markers ◽  
Lower Altitude ◽  
Genetic Lineages ◽  
Genetic Clusters ◽  
Multiple Introduction

Population genetic analyses of the invasive pantropical earthworm P. corethrurus populations will contribute significantly to better understand the ecology and especially the reproductive system of this species. Using 34 polymorphic ISSR markers the genetic diversity and population structure was assessed for four populations of P. corethrurus along an altitudinal gradient, ranging from sea level up to ~1667 meters. Nuclear markers were able to distinguish two genetic clusters, probably corresponding to two distinct genetic lineages, herein defined as A and B. Clones were detected in one population (Actopan at 480 masl) and its number was lower than expected for a parthenogenetic species. Nevertheless, low levels of genetic diversity and a high number of intermediary genotypes were detected among the studied P. corethrurus populations with no apparent population structure related to the distinct geographic regions, which may indicate that human-mediated transference is prevalent, in particular, for the lower altitude regions. Hybridisation between the two genetic clusters was tested and pointed to 11 MLGs as being later-generation hybrids (B1 introgression) mainly associated with the three lower altitude regions. Still, most of the individuals seem to belong to lineage A and only five individuals seem to belong exclusively to the lineage B. Interestingly, these parental individuals were only found present at the highest altitude site, Naolinco (1566-1667 masl), which also showed the highest values of genotypic richness. During the biological invasion, multiple introduction of different genetic lineages can provide opportunities for admixture among genetically distinct clusters. The signatures of admixture among P. corethrurus populations along the altitudinal gradient in Mexico may have allowed the invasion success by directly increasing fitness. ISSR markers revealed to be useful for the study of genetic variation in the invasive pantropical earthworm, P. corethrurus.

scholarly journals Genetic diversity and population structure in an invasive pantropical earthworm along an altitudinal gradient

2017 ◽  
Author(s):  
Diana Ortíz-Gamino ◽  
Luis Cunha ◽  
Esperanza Martínez-Romero ◽  
Norma Flores-Estévez ◽  
Ángel I Ortíz-Ceballos
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Altitudinal Gradient ◽  
Issr Markers ◽  
Invasion Success ◽  
Nuclear Markers ◽  
Lower Altitude ◽  
Genetic Lineages ◽  
Genetic Clusters ◽  
Multiple Introduction

Population genetic analyses of the invasive pantropical earthworm P. corethrurus populations will contribute significantly to better understand the ecology and especially the reproductive system of this species. Using 34 polymorphic ISSR markers the genetic diversity and population structure was assessed for four populations of P. corethrurus along an altitudinal gradient, ranging from sea level up to ~1667 meters. Nuclear markers were able to distinguish two genetic clusters, probably corresponding to two distinct genetic lineages, herein defined as A and B. Clones were detected in one population (Actopan at 480 masl) and its number was lower than expected for a parthenogenetic species. Nevertheless, low levels of genetic diversity and a high number of intermediary genotypes were detected among the studied P. corethrurus populations with no apparent population structure related to the distinct geographic regions, which may indicate that human-mediated transference is prevalent, in particular, for the lower altitude regions. Hybridisation between the two genetic clusters was tested and pointed to 11 MLGs as being later-generation hybrids (B1 introgression) mainly associated with the three lower altitude regions. Still, most of the individuals seem to belong to lineage A and only five individuals seem to belong exclusively to the lineage B. Interestingly, these parental individuals were only found present at the highest altitude site, Naolinco (1566-1667 masl), which also showed the highest values of genotypic richness. During the biological invasion, multiple introduction of different genetic lineages can provide opportunities for admixture among genetically distinct clusters. The signatures of admixture among P. corethrurus populations along the altitudinal gradient in Mexico may have allowed the invasion success by directly increasing fitness. ISSR markers revealed to be useful for the study of genetic variation in the invasive pantropical earthworm, P. corethrurus.

scholarly journals Population genetics and diversity structure of an invasive earthworm in tropical and temperate pastures from Veracruz, Mexico

ZooKeys ◽  
2020 ◽  
Vol 941 ◽  
pp. 49-69
Author(s):  
Diana Ortíz-Gamino ◽  
Josefat Gregorio ◽  
Luis Cunha ◽  
Esperanza Martínez-Romero ◽  
Carlos Fragoso ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Geographic Region ◽  
Apparent Lack ◽  
Genetic Lineages ◽  
Low Genetic Diversity ◽  
Population Structures ◽  
Temperate Pastures

Pontoscolex corethrurus (Müller, 1857) is an invasive tropical earthworm, globally distributed. It reproduces through parthenogenesis, which theoretically results in low genetic diversity. The analysis of the population structure of P. corethrurus using molecular markers may significantly contribute to understanding the ecology and reproductive system of this earthworm species. This work assessed the genetic diversity and population structure of P. corethrurus with 34 polymorphic inter simple sequence repeat markers, covering four populations in tropical and temperate pastures from Veracruz State. Nuclear markers distinguished two genetic clusters, probably corresponding to two distinct genetic lineages. The number of clones detected in the AC population was lower than expected for a parthenogenetic species. Also, the apparent lack of differences in population structures related to the geographic region among the populations studied may indicate that human-mediated transference is prevalent in these areas. Still, most individuals apparently belong to lineage A, and only a few individuals seem to belong to the lineage B. Thus, the admixture signatures found among the four populations of P. corethrurus may have facilitated a successful invasion by directly increasing fitness. In summary, addressing the genetic variation of P. corethrurus with ISSR markers was a suitable approach, as it evidenced the genetic diversity and relationships in the populations evaluated.

Genetic diversity and population structure of the mangrove lime (Merope angulata) in India revealed by AFLP and ISSR markers

2015 ◽  
Vol 120 ◽  
pp. 260-267 ◽  
Author(s):  
Satya Narayan Jena ◽  
Sushma Verma ◽  
Kuttan Narayanan Nair ◽  
Awadhesh Kumar Srivastava ◽  
Sujata Misra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Issr Markers

scholarly journals Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers

2014 ◽  
Vol 13 (3) ◽  
pp. 6340-6349 ◽  
Author(s):  
D.X. Chen ◽  
L.Y. Li ◽  
X. Zhang ◽  
Y. Wang ◽  
Z. Zhang
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Issr Markers

scholarly journals Evaluation of a Diverse, Worldwide Collection of Wild, Cultivated, and Landrace Pepper (Capsicum annuum) for Resistance to Phytophthora Fruit Rot, Genetic Diversity, and Population Structure

2015 ◽  
Vol 105 (1) ◽  
pp. 110-118 ◽  
Author(s):  
R. P. Naegele ◽  
A. J. Tomlinson ◽  
M. K. Hausbeck
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Phytophthora Capsici ◽  
The United States ◽  
Fruit Rot ◽  
Sources Of Resistance ◽  
Plant Introductions ◽  
Genetic Clusters ◽  
Commercial Breeding ◽  
Moderately Resistant

Pepper is the third most important solanaceous crop in the United States and fourth most important worldwide. To identify sources of resistance for commercial breeding, 170 pepper genotypes from five continents and 45 countries were evaluated for Phytophthora fruit rot resistance using two isolates of Phytophthora capsici. Genetic diversity and population structure were assessed on a subset of 157 genotypes using 23 polymorphic simple sequence repeats. Partial resistance and isolate-specific interactions were identified in the population at both 3 and 5 days postinoculation (dpi). Plant introductions (PIs) 640833 and 566811 were the most resistant lines evaluated at 5 dpi to isolates 12889 and OP97, with mean lesion areas less than Criollo de Morelos. Genetic diversity was moderate (0.44) in the population. The program STRUCTURE inferred four genetic clusters with moderate to very great differentiation among clusters. Most lines evaluated were susceptible or moderately susceptible at 5 dpi, and no lines evaluated were completely resistant to Phytophthora fruit rot. Significant population structure was detected when pepper varieties were grouped by predefined categories of disease resistance, continent, and country of origin. Moderately resistant or resistant PIs to both isolates of P. capsici at 5 dpi were in genetic clusters one and two.

scholarly journals Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

2013 ◽  
Vol 26 (3) ◽  
pp. 362-369 ◽  
Author(s):  
Man Jung Kang ◽  
Suresh Sundan ◽  
Gi An Lee ◽  
Ho Cheol Ko ◽  
Jong Wook Chung ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Issr Markers ◽  
Agastache Rugosa

Genetic Diversity and Population Structure of Phyllosticta citriasiana in China

2020 ◽  
Author(s):  
Yibing Zeng ◽  
Tao Xiong ◽  
Bei Liu ◽  
Elma Carstens ◽  
Xiangling Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Mating Type ◽  
Tan Spot ◽  
Long Distance ◽  
High Genetic Diversity ◽  
Geographic Population ◽  
Sexual And Asexual Reproduction ◽  
Geographic Populations ◽  
Genetic Clusters

Phyllosticta citriasiana is the causal agent of citrus tan spot, an important pomelo disease in Asia. At present, there is little or no information on the epidemiology or population structure of P. citriasiana. Using simple sequence repeat (SSR) markers, 94 isolates obtained from three pomelo production regions in southern/southeastern China were analyzed. The analyses showed high genetic diversity in each of the three geographic populations. A STRUCTURE analysis revealed two genetic clusters among the 94 isolates, one geographic population was dominated by genotypes in one cluster while the other two geographic populations were dominated by genotypes of the second cluster. P. citriasiana has a heterothallic mating system with two idiomorphs, MAT1-1 and MAT1-2. Analyses using mating type-specific primers revealed that both mating types were present in all three geographic populations, and in all three populations the mating type ratios were in equilibrium. Although the sexual stage of the fungus has not been discovered yet, analyses of allelic associations indicated evidence for sexual and asexual reproduction within and among populations. Despite the observed genetic differentiation among the three geographic populations, evidence for long-distance gene flow was found.

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