scholarly journals Application of HPV DNA Testing in Follow-up after Loop Electrosurgical Excision Procedures in Northern Thailand

2015 ◽  
Vol 16 (14) ◽  
pp. 6093-6097 ◽  
Author(s):  
Surapan Khunamornpong ◽  
Jongkolnee Settakorn ◽  
Kornkanok Sukpan ◽  
Chumnan Kietpeerakool ◽  
Charuwan Tantipalakorn ◽  
...  
Keyword(s):  
Dna Testing ◽  
Northern Thailand ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Loop Electrosurgical Excision

scholarly journals Comparison of Human Papillomavirus Detection in Urine and Cervical Samples Using High-Risk HPV DNA Testing in Northern Thailand

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Surapan Khunamornpong ◽  
Jongkolnee Settakorn ◽  
Kornkanok Sukpan ◽  
Suree Lekawanvijit ◽  
Narisara Katruang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Testing ◽  
Dna Testing ◽  
Northern Thailand ◽  
Cervical Sample ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hpv Test ◽  
High Risk Hpv

Objective. To evaluate the performance of high-risk human papillomavirus (HPV) DNA testing in urine samples compared to that of cervical sample testing in Northern Thailand. Methods. Paired urine and cervical samples were collected during the follow-up of women with a previous positive HPV test. HPV testing was performed using the Cobas 4800 HPV Test. Linear Array assay was used for genotyping in selected cases. Results. Paired urine and cervical samples were obtained from 168 women. Of 123 paired samples with valid results, agreement in the detection of high-risk HPV DNA was present in 106 cases (86.2%), with a kappa statistic of 0.65 (substantial agreement). Using the cervical HPV results as a reference, the sensitivity of urine HPV testing was 68.6% (24/35) and the specificity 93.2% (82/88). For the detection of histologic high-grade squamous intraepithelial lesion or worse (HSIL+), the sensitivity of urine HPV testing was 80.0% (4/5) and the specificity 78.0% (92/118). Conclusion. Although urine HPV testing had a rather low sensitivity for HPV detection, its sensitivity for histologic HSIL+ detection was high. For clinical use of urine HPV testing, standardization of specimen collection and processing techniques or application of a more sensitive test, especially in the detection of HPV52 and HPV58, is necessary.

Three-Year Follow-up for Women 30 Years and Older with Negative Pap and Negative Hybrid Capture 2 HPV DNA Testing Results

2012 ◽  
Vol 1 (1) ◽  
pp. S40
Author(s):  
Marilyn Dawlett ◽  
Teresa Kologinczak ◽  
Jian-Ping Wang ◽  
Nour Sneige ◽  
Therese Bevers ◽  
...  
Keyword(s):  
Dna Testing ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hybrid Capture 2

scholarly journals Triage of HR-HPV Positive Women with Minor Cytological Abnormalities: A Comparison of mRNA Testing, HPV DNA Testing, and Repeat Cytology Using a 4-Year Follow-Up of a Population-Based Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e90023 ◽  
Author(s):  
Maria Persson ◽  
K. Miriam Elfström ◽  
Sophia Brismar Wendel ◽  
Elisabete Weiderpass ◽  
Sonia Andersson
Keyword(s):  
Population Based ◽  
Dna Testing ◽  
Population Based Study ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Cytological Abnormalities

Cervical Cancer Screening

2008 ◽  
Vol 6 (1) ◽  
pp. 58 ◽  
Author(s):  
_ _
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Current Method ◽  
Dna Testing ◽  
Abnormal Cytology ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Recent Version

Cervical carcinoma remains a health issue for women worldwide. Cervical cytology screening is the current method for early detection, and the NCCN Cervical Cancer Screening Clinical Practice Guidelines in Oncology provide direction for evaluating and managing this process, including clarified and revised recommendations on screening techniques and intervals and follow-up of abnormal screening results, including colposcopy. Human papillomavirus (HPV) DNA testing for primary cervical cancer has been approved by the FDA, and HPV DNA testing for high-risk virus types can also be used as a component of both primary screening and workup of abnormal cytology results. Colposcopy, along with colposcopically directed biopsies, has become the primary method for evaluating women with abnormal cervical cytologies. Special considerations for colposcopy performed during pregnancy are also discussed. For the most recent version of the guidelines, please visit NCCN.org

scholarly journals Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing

2016 ◽  
Vol 17 (5) ◽  
pp. 750 ◽  
Author(s):  
Alex Vorsters ◽  
Severien Van Keer ◽  
Samantha Biesmans ◽  
Annick Hens ◽  
Ilse De Coster ◽  
...  
Keyword(s):  
Hpv Infection ◽  
Dna Testing ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Long Term Follow Up

p16/Ki-67 Immunocytochemistry in Gynecological Cytology: Limitations in Practice

2017 ◽  
Vol 61 (3) ◽  
pp. 230-236 ◽  
Author(s):  
Peter Ziemke
Keyword(s):  
Risk Assessment ◽  
High Risk ◽  
Positive Result ◽  
Dna Testing ◽  
Ki 67 ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Immunochemical Detection ◽  
High Risk Hpv

Objective: Immunochemical detection of the protein p16INK4a in cervical cytology is used in combination with Ki-67. Cells positive for both proteins are certain to have been transformed by high-risk HPV. p16/Ki-67 immunocytochemistry provides a significant improvement in specificity over cytology and HPV DNA testing. However, p16/Ki-67 immunocytochemistry also has its limitations. Study Design: The research is based on the follow-up of 1,131 patients for whom p16/Ki-67 immunocytochemistry was performed with cytology. Dependencies on the age of patients with LSIL, number of p16/Ki-67-positive cells, and different results during repeated examinations were analyzed. Results: In LSIL, positive p16/Ki-67 is less specific for ≥CIN2/HSIL for patients younger than 30 years compared to patients aged 30 years or older (61.1 vs. 75.7%, p < 0.013). Using a score of 10 p16/Ki-67-marked cells as a positive result instead of 1 led to significantly higher specificity (89.0 vs. 70.2%, p < 0.001). This modified threshold offers better risk assessment in LSIL. In repeated immunocytochemical investigations, 28.4% of the results deviated from the first examination. Conclusion: The abovementioned discrepancies can be interpreted as hints about the molecular biological causes of suboptimal performance of p16/Ki-67. An efficient and reliable application of p16/Ki-67 immunocytochemistry requires knowledge of its methodological limitations.

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