scholarly journals Author response: Real-time observation of signal recognition particle binding to actively translating ribosomes

2014 ◽  
Author(s):  
Thomas R Noriega ◽  
Jin Chen ◽  
Peter Walter ◽  
Joseph D Puglisi
eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Thomas R Noriega ◽  
Jin Chen ◽  
Peter Walter ◽  
Joseph D Puglisi

The signal recognition particle (SRP) directs translating ribosome-nascent chain complexes (RNCs) that display a signal sequence to protein translocation channels in target membranes. All previous work on the initial step of the targeting reaction, when SRP binds to RNCs, used stalled and non-translating RNCs. This meant that an important dimension of the co-translational process remained unstudied. We apply single-molecule fluorescence measurements to observe directly and in real-time E. coli SRP binding to actively translating RNCs. We show at physiologically relevant SRP concentrations that SRP-RNC association and dissociation rates depend on nascent chain length and the exposure of a functional signal sequence outside the ribosome. Our results resolve a long-standing question: how can a limited, sub-stoichiometric pool of cellular SRP effectively distinguish RNCs displaying a signal sequence from those that are not? The answer is strikingly simple: as originally proposed, SRP only stably engages translating RNCs exposing a functional signal sequence.


Author(s):  
K. Harada ◽  
T. Matsuda ◽  
J.E. Bonevich ◽  
M. Igarashi ◽  
S. Kondo ◽  
...  

Previous observations of magnetic flux-lines (vortex lattices) in superconductors, such as the field distribution of a flux-line, and flux-line dynamics activated by heat and current, have employed the high spatial resolution and magnetic sensitivity of electron holography. And recently, the 2-D static distribution of vortices was also observed by this technique. However, real-time observations of the vortex lattice, in spite of scientific and technological interest, have not been possible due to experimental difficulties. Here, we report the real-time observation of vortex lattices in a thin superconductor, by means of Lorentz microscopy using a 300 kV field emission electron microscope. This technique allows us to observe the dynamic motion of individual vortices and record the events on a VTR system.The experimental arrangement is shown in Fig. 1. A Nb thin film for transmission observation was prepared by chemical etching. The grain size of the film was increased by annealing, and single crystals were observed with a thickness of 50∼90 nm.


1997 ◽  
Vol 491 (2) ◽  
pp. 436-450 ◽  
Author(s):  
C. Alcock ◽  
W. H. Allen ◽  
R. A. Allsman ◽  
D. Alves ◽  
T. S. Axelrod ◽  
...  

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Jiaqi Zhou ◽  
Chaoxiong He ◽  
Ming-Ming Liu ◽  
Enliang Wang ◽  
Shaokui Jia ◽  
...  

2021 ◽  
pp. 103786
Author(s):  
Dongdong Han ◽  
Yijie Wang ◽  
Zhanqiang Hui ◽  
Zhixing Zhang ◽  
Kaili Ren ◽  
...  

2012 ◽  
Vol 100 (19) ◽  
pp. 193702 ◽  
Author(s):  
Mikio Kato ◽  
Walter Meissl ◽  
Kenji Umezawa ◽  
Tokihiro Ikeda ◽  
Yasunori Yamazaki

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