scholarly journals Decision letter: EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells

2014 ◽  
Keyword(s):  
Stem Cells ◽  
Cell Polarity ◽  
Egfr Signaling ◽  
Self Renewal ◽  
Follicle Stem Cells

scholarly journals Wingless promotes EGFR signaling in follicle stem cells to maintain self-renewal

Development ◽  
2018 ◽  
Vol 145 (23) ◽  
pp. dev168716 ◽  
Author(s):  
Rebecca P. Kim-Yip ◽  
Todd G. Nystul
Keyword(s):  
Stem Cells ◽  
Egfr Signaling ◽  
Self Renewal ◽  
Follicle Stem Cells

scholarly journals EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Angela Castanieto ◽  
Michael J Johnston ◽  
Todd G Nystul
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Polarity ◽  
Growth Factor Receptor ◽  
Egfr Signaling ◽  
Epithelial Stem Cells ◽  
Cell Fates ◽  
Liver Kinase B1 ◽  
Follicle Stem Cells ◽  
Drosophila Ovary

Epithelial stem cells divide asymmetrically, such that one daughter replenishes the stem cell pool and the other differentiates. We found that, in the epithelial follicle stem cell (FSC) lineage of the Drosophila ovary, epidermal growth factor receptor (EGFR) signaling functions specifically in the FSCs to promote the unique partially polarized state of the FSC, establish apical–basal polarity throughout the lineage, and promote FSC maintenance in the niche. In addition, we identified a novel connection between EGFR signaling and the cell-polarity regulator liver kinase B1 (LKB1), which indicates that EGFR signals through both the Ras–Raf–MEK–Erk pathway and through the LKB1–AMPK pathway to suppress apical identity. The development of apical–basal polarity is the earliest visible difference between FSCs and their daughters, and our findings demonstrate that the EGFR-mediated regulation of apical–basal polarity is essential for the segregation of stem cell and daughter cell fates.

scholarly journals Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Cuie Chen ◽  
Ryan Cummings ◽  
Aghapi Mordovanakis ◽  
Alan J Hunt ◽  
Michael Mayer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Cell Fate ◽  
Cell Polarity ◽  
Adult Stem Cells ◽  
Asymmetric Cell Division ◽  
Cytokine Receptor ◽  
Spindle Orientation ◽  
Germline Stem Cells ◽  
Self Renewal

Asymmetric stem cell division is a critical mechanism for balancing self-renewal and differentiation. Adult stem cells often orient their mitotic spindle to place one daughter inside the niche and the other outside of it to achieve asymmetric division. It remains unknown whether and how the niche may direct division orientation. Here we discover a novel and evolutionary conserved mechanism that couples cell polarity to cell fate. We show that the cytokine receptor homolog Dome, acting downstream of the niche-derived ligand Upd, directly binds to the microtubule-binding protein Eb1 to regulate spindle orientation in Drosophila male germline stem cells (GSCs). Dome’s role in spindle orientation is entirely separable from its known function in self-renewal mediated by the JAK-STAT pathway. We propose that integration of two functions (cell polarity and fate) in a single receptor is a key mechanism to ensure an asymmetric outcome following cell division.

scholarly journals Integrins control the positioning and proliferation of follicle stem cells in the Drosophila ovary

2008 ◽  
Vol 182 (4) ◽  
pp. 801-815 ◽  
Author(s):  
Alana M. O'Reilly ◽  
Hsiu-Hsiang Lee ◽  
Michael A. Simon
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adult Stem Cells ◽  
Cell Communication ◽  
Self Renewal ◽  
Ovarian Stem Cells ◽  
Stem Cell Populations ◽  
Integrin Ligand ◽  
Follicle Stem Cells ◽  
Drosophila Ovary

Adult stem cells are maintained in specialized microenvironments called niches, which promote self-renewal and prevent differentiation. In this study, we show that follicle stem cells (FSCs) in the Drosophila melanogaster ovary rely on cues that are distinct from those of other ovarian stem cells to establish and maintain their unique niche. We demonstrate that integrins anchor FSCs to the basal lamina, enabling FSCs to maintain their characteristic morphology and position. Integrin-mediated FSC anchoring is also essential for proper development of differentiating prefollicle cells that arise from asymmetrical FSC divisions. Our results support a model in which FSCs contribute to the formation and maintenance of their own niche by producing the integrin ligand, laminin A (LanA). Together, LanA and integrins control FSC proliferation rates, a role that is separable from their function in FSC anchoring. Importantly, LanA-integrin function is not required to maintain other ovarian stem cell populations, demonstrating that distinct pathways regulate niche–stem cell communication within the same organ.

scholarly journals Arl2- and Msps-dependent microtubule growth governs asymmetric division

2016 ◽  
Vol 212 (6) ◽  
pp. 661-676 ◽  
Author(s):  
Keng Chen ◽  
Chwee Tat Koe ◽  
Zhanyuan Benny Xing ◽  
Xiaolin Tian ◽  
Fabrizio Rossi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cell Polarity ◽  
Control Cell ◽  
Asymmetric Division ◽  
New Paradigm ◽  
Self Renewal ◽  
Tubulin Binding ◽  
Microtubule Growth ◽  
Adp Ribosylation Factor

Asymmetric division of neural stem cells is a fundamental strategy to balance their self-renewal and differentiation. It is long thought that microtubules are not essential for cell polarity in asymmetrically dividing Drosophila melanogaster neuroblasts (NBs; neural stem cells). Here, we show that Drosophila ADP ribosylation factor like-2 (Arl2) and Msps, a known microtubule-binding protein, control cell polarity and spindle orientation of NBs. Upon arl2 RNA intereference, Arl2-GDP expression, or arl2 deletions, microtubule abnormalities and asymmetric division defects were observed. Conversely, overactivation of Arl2 leads to microtubule overgrowth and depletion of NBs. Arl2 regulates microtubule growth and asymmetric division through localizing Msps to the centrosomes in NBs. Moreover, Arl2 regulates dynein function and in turn centrosomal localization of D-TACC and Msps. Arl2 physically associates with tubulin cofactors C, D, and E. Arl2 functions together with tubulin-binding cofactor D to control microtubule growth, Msps localization, and NB self-renewal. Therefore, Arl2- and Msps-dependent microtubule growth is a new paradigm regulating asymmetric division of neural stem cells.

scholarly journals Distinct Self-Renewal and Differentiation Phases in the Niche of Infrequently Dividing Hair Follicle Stem Cells

2009 ◽  
Vol 5 (3) ◽  
pp. 267-278 ◽  
Author(s):  
Ying V. Zhang ◽  
Janice Cheong ◽  
Nichita Ciapurin ◽  
David J. McDermitt ◽  
Tudorita Tumbar
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Self Renewal ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division

2017 ◽  
Author(s):  
Cuie Chen ◽  
Ryan Cummings ◽  
Aghapi Mordovanakis ◽  
Alan J. Hunt ◽  
Michael Mayer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Cell Fate ◽  
Cell Polarity ◽  
Adult Stem Cells ◽  
Asymmetric Cell Division ◽  
Cytokine Receptor ◽  
Spindle Orientation ◽  
Germline Stem Cells ◽  
Self Renewal

AbstractAsymmetric stem cell division is a critical mechanism for balancing self-renewal and differentiation. Adult stem cells often orient their mitotic spindle to place one daughter inside the niche and the other outside of it to achieve asymmetric division. It remains unknown whether and how the niche may direct division orientation. Here we discover a novel and evolutionary conserved mechanism that couples cell polarity to cell fate. We show that the cytokine receptor homolog Dome, acting downstream of the niche-derived ligand Upd, directly binds to the microtubule-binding protein Eb1 to regulate spindle orientation in Drosophila male germline stem cells (GSCs). Dome’s role in spindle orientation is entirely separable from its known function in self-renewal mediated by the JAK-STAT pathway. We propose that integration of two functions (cell polarity and fate) in a single receptor is a key mechanism to ensure an asymmetric outcome following cell division.

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