scholarly journals Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Hajime Fukui ◽  
Takahiro Miyazaki ◽  
Renee Wei-Yan Chow ◽  
Hiroyuki Ishikawa ◽  
Hiroyuki Nakajima ◽  
...  
Keyword(s):  
Cell Number ◽  
Bmp Signaling ◽  
Hippo Signaling ◽  
Lateral Plate Mesoderm ◽  
Cardiomyocyte Proliferation ◽  
Heart Tube ◽  
Lateral Plate ◽  
Large Tumor ◽  
Developmental Potential ◽  
Anterior Lateral Plate Mesoderm

The differentiation of the lateral plate mesoderm cells into heart field cells constitutes a critical step in the development of cardiac tissue and the genesis of functional cardiomyocytes. Hippo signaling controls cardiomyocyte proliferation, but the role of Hippo signaling during early cardiogenesis remains unclear. Here, we show that Hippo signaling regulates atrial cell number by specifying the developmental potential of cells within the anterior lateral plate mesoderm (ALPM), which are incorporated into the venous pole of the heart tube and ultimately into the atrium of the heart. We demonstrate that Hippo signaling acts through large tumor suppressor kinase 1/2 to modulate BMP signaling and the expression of hand2, a key transcription factor that is involved in the differentiation of atrial cardiomyocytes. Collectively, these results demonstrate that Hippo signaling defines venous pole cardiomyocyte number by modulating both the number and the identity of the ALPM cells that will populate the atrium of the heart.

scholarly journals Hippo signaling restricts cells in the second heart field that differentiate into Islet-1-positive atrial cardiomyocytes

2017 ◽  
Author(s):  
Hajime Fukui ◽  
Takahiro Miyazaki ◽  
Hiroyuki Ishikawa ◽  
Hiroyuki Nakajima ◽  
Naoki Mochizuki
Keyword(s):  
Hippo Signaling ◽  
Lateral Plate ◽  
Large Tumor ◽  
Atrial Cardiomyocytes ◽  
Second Heart Field ◽  
Kinase Cascade ◽  
Heart Field ◽  
Heart Formation ◽  
First Heart Field ◽  
Islet 1

AbstractCardiac precursor cells (CPCs) in the first heart field (FHF) and the second heart field (SHF) present at both arterial and venous poles assemble to form a cardiac tube in zebrafish. Hippo kinase cascade is essential for proper heart formation; however, it remains elusive how Hippo signal contributes to early cardiac fate determination. We here demonstrate that mutants of large tumor suppressor kinase 1/2 (lats1/2) exhibited an increase in a SHF marker, Islet1 (Isl1)-positive and hand2 promoter-activated venous pole atrial cardiomyocytes (CMs) and that those showed expansion of the domain between between the anterior and the posterior lateral plate mesoderm. Consistently, TEAD-8 dependent transcription was activated in caudal region of the left ALPM cells that gave rise to the venous pole atrial CMs. Yap1/Wwtr1-promoted bmp2b expression was essential for Smad-regulated hand2 expression in the left ALPM, indicating that Hippo signaling restricts the SHF cells originating from the left ALPM that move toward the venous pole.

Not Just Inductive: A Critical Mechanical Role for the Endoderm During Heart Tube Assembly

2012 ◽  
Author(s):  
Victor D. Varner ◽  
Larry A. Taber
Keyword(s):  
Close Contact ◽  
Ventral Side ◽  
Lateral Plate Mesoderm ◽  
Heart Tube ◽  
Lateral Plate ◽  
Cardiac Progenitors ◽  
Cardiac Specification ◽  
Tube Assembly ◽  
Heart Fields

The heart is the first functioning organ to form during development. Similar to other organ primordia, the embryonic heart forms as a simple tube — in this case, a straight muscle-wrapped tube situated on the ventral side of the embryo. During gastrulation, the cardiac progenitors reside in the lateral plate mesoderm but maintain close contact with the underlying endoderm. In amniotes, these bilateral heart fields are initially organized as a pair of flat epithelia that move toward the embryonic midline and fuse above the anterior intestinal portal (AIP) to form the heart tube. This medial motion is typically attributed to active mesodermal migration over the underlying endoderm. In this view, the role of the endoderm is two-fold: to serve as a mechanically passive substrate for the crawling mesoderm and to secrete various growth factors necessary for cardiac specification and differentiation.

scholarly journals Multi-modal effects of BMP signaling on Nodal expression in the lateral plate mesoderm during left–right axis formation in the chick embryo

2013 ◽  
Vol 374 (1) ◽  
pp. 71-84 ◽  
Author(s):  
Kenjiro Katsu ◽  
Norifumi Tatsumi ◽  
Daisuke Niki ◽  
Ken-ichi Yamamura ◽  
Yuji Yokouchi
Keyword(s):  
Chick Embryo ◽  
Bmp Signaling ◽  
Axis Formation ◽  
Lateral Plate Mesoderm ◽  
Lateral Plate

scholarly journals Somite Compartments in Amphioxus and Its Implications on the Evolution of the Vertebrate Skeletal Tissues

2021 ◽  
Vol 9 ◽  
Author(s):  
Luok Wen Yong ◽  
Tsai-Ming Lu ◽  
Che-Huang Tung ◽  
Ruei-Jen Chiou ◽  
Kun-Lung Li ◽  
...  
Keyword(s):  
Neural Tube ◽  
Developmental Process ◽  
Lateral Wall ◽  
Bmp Signaling ◽  
Lateral Plate Mesoderm ◽  
Evolutionary Novelty ◽  
Lateral Plate ◽  
Collagenous Matrix ◽  
Previous Hypothesis ◽  
Collagen Genes

Mineralized skeletal tissues of vertebrates are an evolutionary novelty within the chordate lineage. While the progenitor cells that contribute to vertebrate skeletal tissues are known to have two embryonic origins, the mesoderm and neural crest, the evolutionary origin of their developmental process remains unclear. Using cephalochordate amphioxus as our model, we found that cells at the lateral wall of the amphioxus somite express SPARC (a crucial gene for tissue mineralization) and various collagen genes. During development, some of these cells expand medially to surround the axial structures, including the neural tube, notochord and gut, while others expand laterally and ventrally to underlie the epidermis. Eventually these cell populations are found closely associated with the collagenous matrix around the neural tube, notochord, and dorsal aorta, and also with the dense collagen sheets underneath the epidermis. Using known genetic markers for distinct vertebrate somite compartments, we showed that the lateral wall of amphioxus somite likely corresponds to the vertebrate dermomyotome and lateral plate mesoderm. Furthermore, we demonstrated a conserved role for BMP signaling pathway in somite patterning of both amphioxus and vertebrates. These results suggest that compartmentalized somites and their contribution to primitive skeletal tissues are ancient traits that date back to the chordate common ancestor. The finding of SPARC-expressing skeletal scaffold in amphioxus further supports previous hypothesis regarding SPARC gene family expansion in the elaboration of the vertebrate mineralized skeleton.

scholarly journals Zebrafish second heart field development relies on progenitor specification in anterior lateral plate mesoderm and nkx2.5 function

Development ◽  
2013 ◽  
Vol 140 (6) ◽  
pp. 1353-1363 ◽  
Author(s):  
B. Guner-Ataman ◽  
N. Paffett-Lugassy ◽  
M. S. Adams ◽  
K. R. Nevis ◽  
L. Jahangiri ◽  
...  
Keyword(s):  
Lateral Plate Mesoderm ◽  
Lateral Plate ◽  
Field Development ◽  
Second Heart Field ◽  
Heart Field ◽  
Anterior Lateral Plate Mesoderm

BMP2 is a positive regulator of Nodal signaling during left-right axis formation in the chicken embryo

Development ◽  
2002 ◽  
Vol 129 (14) ◽  
pp. 3421-3429
Author(s):  
Thomas Schlange ◽  
Hans-Henning Arnold ◽  
Thomas Brand
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Target Genes ◽  
Bmp Signaling ◽  
Paraxial Mesoderm ◽  
Axis Formation ◽  
Nodal Signaling ◽  
Lateral Plate Mesoderm ◽  
Lateral Plate ◽  
Positive Regulator ◽  
The Right

A model of left-right axis formation in the chick involves inhibition of bone morphogenetic proteins by the antagonist Car as a mechanism of upregulating Nodal in the left lateral plate mesoderm. By contrast, expression of CFC, a competence factor, which is absolutely required for Nodal signaling in the lateral plate mesoderm is dependent on a functional BMP signaling pathway. We have therefore investigated the relationship between BMP and Nodal in further detail. We implanted BMP2 and Noggin-expressing cells into the left lateral plate and paraxial mesoderm and observed a strong upregulation of Nodal and its target genes Pitx2 and Nkx3.2. In addition Cfc, the Nodal type II receptor ActrIIa and Snr were found to depend on BMP signaling for their expression. Comparison of the expression domains of Nodal, Bmp2, Car and Cfc revealed co-expression of Nodal, Cfc and Bmp2, while Car and Nodal only partially overlapped. Ectopic application of BMP2, Nodal, and Car as well as combinations of this signaling molecules to the right lateral plate mesoderm revealed that BMP2 and Car need to synergize in order to specify left identity. We propose a novel model of left-right axis formation, which involves BMP as a positive regulator of Nodal signaling in the chick embryo.

Sign in / Sign up

Export Citation Format

Share Document