scholarly journals Zebrafish second heart field development relies on progenitor specification in anterior lateral plate mesoderm and nkx2.5 function

Development ◽  
2013 ◽  
Vol 140 (6) ◽  
pp. 1353-1363 ◽  
Author(s):  
B. Guner-Ataman ◽  
N. Paffett-Lugassy ◽  
M. S. Adams ◽  
K. R. Nevis ◽  
L. Jahangiri ◽  
...  
Keyword(s):  
Lateral Plate Mesoderm ◽  
Lateral Plate ◽  
Field Development ◽  
Second Heart Field ◽  
Heart Field ◽  
Anterior Lateral Plate Mesoderm

scholarly journals Persistent Ventricle Partitioning in the Adult Zebrafish Heart

2021 ◽  
Vol 8 (4) ◽  
pp. 41
Author(s):  
Catherine Pfefferli ◽  
Hannah R. Moran ◽  
Anastasia Felker ◽  
Christian Mosimann ◽  
Anna Jaźwińska
Keyword(s):  
Lateral Plate Mesoderm ◽  
Adult Zebrafish ◽  
Lateral Plate ◽  
Second Heart Field ◽  
Heart Field ◽  
Left And Right ◽  
First Heart Field ◽  
The Right ◽  
Physical Boundary ◽  
Zebrafish Heart

The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF), both emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling using drl-driven CreERT2 recombinase with a myocardium-specific, myl7-controlled, loxP reporter results in the predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary.

scholarly journals Persistent ventricle partitioning in the adult zebrafish heart

2021 ◽  
Author(s):  
Catherine Pfefferli ◽  
Hannah R. Moran ◽  
Anastasia Felker ◽  
Christian Mosimann ◽  
Anna Jazwinska
Keyword(s):  
Lateral Plate Mesoderm ◽  
Adult Zebrafish ◽  
Lateral Plate ◽  
Second Heart Field ◽  
Heart Field ◽  
Left And Right ◽  
First Heart Field ◽  
The Right ◽  
Physical Boundary ◽  
Zebrafish Heart

The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF) emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling using drl-driven CreERT2 recombinase with a myocardium-specific, myl7-controlled loxP reporter results in predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary.

scholarly journals Signaling Pathways Controlling Second Heart Field Development

2009 ◽  
Vol 104 (8) ◽  
pp. 933-942 ◽  
Author(s):  
Francesca Rochais ◽  
Karim Mesbah ◽  
Robert G. Kelly
Keyword(s):  
Signaling Pathways ◽  
Field Development ◽  
Second Heart Field ◽  
Heart Field

scholarly journals Hippo signaling restricts cells in the second heart field that differentiate into Islet-1-positive atrial cardiomyocytes

2017 ◽  
Author(s):  
Hajime Fukui ◽  
Takahiro Miyazaki ◽  
Hiroyuki Ishikawa ◽  
Hiroyuki Nakajima ◽  
Naoki Mochizuki
Keyword(s):  
Hippo Signaling ◽  
Lateral Plate ◽  
Large Tumor ◽  
Atrial Cardiomyocytes ◽  
Second Heart Field ◽  
Kinase Cascade ◽  
Heart Field ◽  
Heart Formation ◽  
First Heart Field ◽  
Islet 1

AbstractCardiac precursor cells (CPCs) in the first heart field (FHF) and the second heart field (SHF) present at both arterial and venous poles assemble to form a cardiac tube in zebrafish. Hippo kinase cascade is essential for proper heart formation; however, it remains elusive how Hippo signal contributes to early cardiac fate determination. We here demonstrate that mutants of large tumor suppressor kinase 1/2 (lats1/2) exhibited an increase in a SHF marker, Islet1 (Isl1)-positive and hand2 promoter-activated venous pole atrial cardiomyocytes (CMs) and that those showed expansion of the domain between between the anterior and the posterior lateral plate mesoderm. Consistently, TEAD-8 dependent transcription was activated in caudal region of the left ALPM cells that gave rise to the venous pole atrial CMs. Yap1/Wwtr1-promoted bmp2b expression was essential for Smad-regulated hand2 expression in the left ALPM, indicating that Hippo signaling restricts the SHF cells originating from the left ALPM that move toward the venous pole.

scholarly journals Author response: Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish

2017 ◽  
Author(s):  
Hajime Fukui ◽  
Takahiro Miyazaki ◽  
Renee Wei-Yan Chow ◽  
Hiroyuki Ishikawa ◽  
Hiroyuki Nakajima ◽  
...  
Keyword(s):  
Author Response ◽  
Hippo Signaling ◽  
Lateral Plate Mesoderm ◽  
Lateral Plate ◽  
Pole Cells ◽  
Anterior Lateral Plate Mesoderm

scholarly journals Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Hajime Fukui ◽  
Takahiro Miyazaki ◽  
Renee Wei-Yan Chow ◽  
Hiroyuki Ishikawa ◽  
Hiroyuki Nakajima ◽  
...  
Keyword(s):  
Cell Number ◽  
Bmp Signaling ◽  
Hippo Signaling ◽  
Lateral Plate Mesoderm ◽  
Cardiomyocyte Proliferation ◽  
Heart Tube ◽  
Lateral Plate ◽  
Large Tumor ◽  
Developmental Potential ◽  
Anterior Lateral Plate Mesoderm

The differentiation of the lateral plate mesoderm cells into heart field cells constitutes a critical step in the development of cardiac tissue and the genesis of functional cardiomyocytes. Hippo signaling controls cardiomyocyte proliferation, but the role of Hippo signaling during early cardiogenesis remains unclear. Here, we show that Hippo signaling regulates atrial cell number by specifying the developmental potential of cells within the anterior lateral plate mesoderm (ALPM), which are incorporated into the venous pole of the heart tube and ultimately into the atrium of the heart. We demonstrate that Hippo signaling acts through large tumor suppressor kinase 1/2 to modulate BMP signaling and the expression of hand2, a key transcription factor that is involved in the differentiation of atrial cardiomyocytes. Collectively, these results demonstrate that Hippo signaling defines venous pole cardiomyocyte number by modulating both the number and the identity of the ALPM cells that will populate the atrium of the heart.

scholarly journals Isl2b regulates anterior second heart field development in zebrafish

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hagen R. Witzel ◽  
Sirisha Cheedipudi ◽  
Rui Gao ◽  
Didier Y. R. Stainier ◽  
Gergana D. Dobreva
Keyword(s):  
Field Development ◽  
Second Heart Field ◽  
Heart Field
Sign in / Sign up

Export Citation Format

Share Document