Author response: Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle

How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer’s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.

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Asymmetric cell volume changes regulate epithelial morphogenesis in zebrafish Kupffer’s vesicle

10.1101/175679 ◽

2017 ◽

Author(s):

Agnik Dasgupta ◽

Matthias Merkel ◽

Andrew E. Jacob ◽

Jonathan Dawson ◽

M. Lisa Manning ◽

...

Keyword(s):

Cell Volume ◽

Cell Shape ◽

Single Cells ◽

Ion Flux ◽

Epithelial Morphogenesis ◽

Volume Changes ◽

Kupffer’S Vesicle ◽

Kupffer's Vesicle ◽

Cell Shape Changes ◽

Shape Changes

ABSTRACTHow epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer's vesicle (KV), a transient organ with a fluid - filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3 - dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates asymmetric cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen - generated forces.

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Decision letter: Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer’s vesicle

10.7554/elife.30963.036 ◽

2017 ◽

Keyword(s):

Cell Volume ◽

Epithelial Morphogenesis ◽

Volume Changes ◽

Kupffer’S Vesicle ◽

Kupffer's Vesicle

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Liver cell hydration and integrin signaling

Biological Chemistry ◽

10.1515/hsz-2021-0193 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Michele Bonus ◽

Dieter Häussinger ◽

Holger Gohlke

Keyword(s):

Cell Volume ◽

Liver Cell ◽

Cell Function ◽

The Other ◽

Signaling Cascades ◽

Integrin Signaling ◽

Volume Changes ◽

The One ◽

And Oxidative Stress

Abstract Liver cell hydration (cell volume) is dynamic and can change within minutes under the influence of hormones, nutrients, and oxidative stress. Such volume changes were identified as a novel and important modulator of cell function. It provides an early example for the interaction between a physical parameter (cell volume) on the one hand and metabolism, transport, and gene expression on the other. Such events involve mechanotransduction (osmosensing) which triggers signaling cascades towards liver function (osmosignaling). This article reviews our own work on this topic with emphasis on the role of β1 integrins as (osmo-)mechanosensors in the liver, but also on their role in bile acid signaling.

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