scholarly journals In vivo detection of optically-evoked opioid peptide release

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Ream Al-Hasani ◽  
Jenny-Marie T Wong ◽  
Omar S Mabrouk ◽  
Jordan G McCall ◽  
Gavin P Schmitz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Opioid Peptide ◽  
Neuronal Circuitry ◽  
Endogenous Peptide ◽  
In Vivo Detection ◽  
Highly Sensitive ◽  
Peptide Release ◽  
Freely Moving ◽  
The Brain

Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.

scholarly journals In vivo detection of optically-evoked opioid peptide release

2018 ◽  
Author(s):  
Ream Al-Hasanil ◽  
Jenny-Marie T. Wong ◽  
Omar S. Mabrouk ◽  
Jordan G. McCall ◽  
Gavin P. Schmitz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Opioid Peptide ◽  
Neuronal Circuitry ◽  
Endogenous Peptide ◽  
In Vivo Detection ◽  
Highly Sensitive ◽  
Peptide Release ◽  
Freely Moving ◽  
The Brain

AbstractThough the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.

scholarly journals Author response: In vivo detection of optically-evoked opioid peptide release

2018 ◽  
Author(s):  
Ream Al-Hasani ◽  
Jenny-Marie T Wong ◽  
Omar S Mabrouk ◽  
Jordan G McCall ◽  
Gavin P Schmitz ◽  
...  
Keyword(s):  
Opioid Peptide ◽  
Author Response ◽  
In Vivo Detection ◽  
Peptide Release

scholarly journals Decision letter: In vivo detection of optically-evoked opioid peptide release

2018 ◽  
Author(s):  
Julie A Kauer ◽  
Christopher Evans ◽  
Richard E Mains
Keyword(s):  
Opioid Peptide ◽  
In Vivo Detection ◽  
Peptide Release

Electrochemistry in vivo: Monitoring dopamine release in the brain of the conscious, freely moving rat

1987 ◽  
Vol 19 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Ross F. Lane ◽  
Charles D. Blaha ◽  
Siva P. Hari
Keyword(s):  
Dopamine Release ◽  
In Vivo Monitoring ◽  
Freely Moving ◽  
The Brain

Noninvasive Detection of Misfolded Proteins in the Brain Using [11C]BF-227 PET

2013 ◽  
pp. 438-445
Author(s):  
Nobuyuki Okamura ◽  
Shozo Furumoto ◽  
Manabu Tashiro ◽  
Katsutoshi Furukawa ◽  
Hiroyuki Arai ◽  
...  
Keyword(s):  
Protein Misfolding ◽  
Protein A ◽  
Lewy Bodies ◽  
Protein Aggregates ◽  
Brain Regions ◽  
Postmortem Brain ◽  
In Vivo Detection ◽  
Misfolding Diseases ◽  
The Brain

Alzheimer’s disease (AD) and many other neurodegenerative disorders belong to the family of protein misfolding diseases. These diseases are characterized by the deposition of insoluble protein aggregates containing an enriched ß-sheet structure. To evaluate PET amyloid-imaging tracer [11C]BF-227 as an agent for in vivo detection of various kinds of misfolded protein, a [11C]BF-227 PET study was performed in patients with various protein misfolding diseases, including AD, frontotemporal dementia (FTD), dementia with Lewy bodies (DLB), sporadic Creutzfeldt-Jakob disease (sCJD) and Gerstmann-Sträussler-Scheinker disease (GSS). BF-227 binds to ß-amyloid fibrils with high affinity. Most of the AD patients showed prominent retention of [11C]BF-227 in the neocortex. In addition, neocortical retention of BF-227 was observed in the subjects with mild cognitive impairment who converted to AD during follow-up. DLB patients had elevated [11C]BF-227 uptake in the neocortex. However, FTD and sCJD patients showed no cortical retention of [11C]BF-227. Patients with multiple system atrophy had elevated BF-227 binding in the putamen. Finally, GSS patients had elevated BF-227 uptake in the cerebellum and other brain regions. This chapter confirms that BF-227 can selectively bind to a-synuclein and prion protein deposits using postmortem brain samples. Based on these findings, [11C]BF-227 is not necessarily specific for ß-amyloid in AD patients. However, this tracer could be used to detect various types of protein aggregates in the brain.

scholarly journals A Review of Neurotransmitters Sensing Methods for Neuro-Engineering Research

2019 ◽  
Vol 9 (21) ◽  
pp. 4719 ◽  
Author(s):  
Shimwe Dominique Niyonambaza ◽  
Praveen Kumar ◽  
Paul Xing ◽  
Jessy Mathault ◽  
Paul De Koninck ◽  
...  
Keyword(s):  
Imaging Techniques ◽  
Ongoing Research ◽  
Engineering Research ◽  
Detection Techniques ◽  
In Vivo Detection ◽  
Low Concentrations ◽  
The Subject ◽  
The Brain

Neurotransmitters as electrochemical signaling molecules are essential for proper brain function and their dysfunction is involved in several mental disorders. Therefore, the accurate detection and monitoring of these substances are crucial in brain studies. Neurotransmitters are present in the nervous system at very low concentrations, and they mixed with many other biochemical molecules and minerals, thus making their selective detection and measurement difficult. Although numerous techniques to do so have been proposed in the literature, neurotransmitter monitoring in the brain is still a challenge and the subject of ongoing research. This article reviews the current advances and trends in neurotransmitters detection techniques, including in vivo sampling and imaging techniques, electrochemical and nano-object sensing techniques for in vitro and in vivo detection, as well as spectrometric, analytical and derivatization-based methods mainly used for in vitro research. The document analyzes the strengths and weaknesses of each method, with the aim to offer selection guidelines for neuro-engineering research.

A Novel Imaging Probe for In Vivo Detection of Neuritic and Diffuse Amyloid Plaques in the Brain

2004 ◽  
Vol 24 (2) ◽  
pp. 247-256 ◽  
Author(s):  
Nobuyuki Okamura ◽  
Takahiro Suemoto ◽  
Tsuyoshi Shiomitsu ◽  
Masako Suzuki ◽  
Hiroshi Shimadzu ◽  
...  
Keyword(s):  
Amyloid Plaques ◽  
Imaging Probe ◽  
In Vivo Detection ◽  
The Brain

Sensitive enzyme electrodes

1990 ◽  
Vol 333 (1628) ◽  
pp. 49-61 ◽  
Keyword(s):  
Packed Bed ◽  
Current Time ◽  
Enzyme Electrodes ◽  
Freely Moving ◽  
The Brain ◽  
Nanomolar Range ◽  
Inhibited Enzyme ◽  
Micromolar Range

Conventional enzyme electrodes are relatively insensitive devices capable of measuring analytes in the micromolar range. Inhibited enzyme electrodes work by measuring the inhibition of an enzyme turning over undersaturated conditions. This increased turnover gives greater sensitivity. The detection limits are controlled either by the thermodynamic amplitude or by the kinetic discrimination. Software has been developed to analyse the current time transient to produce concentrations of the inhibitor. Results for CN- and H 2 S are presented. The packed bed wall jet electrode is an electrode assembly that allows complete reaction of the substrate with the enzyme coupled to an efficient hydrodynamic régime for electrochemical detection. Results for the determination of acetylcholine are presented. The electrode can also be used in an immunoassay for the determination of human immunoglobulin in the nanomolar range. Finally results will be presented for in vivo changes in ascorbate in the brain of the freely moving rat as a result of tail pinch; changes on a timescale of half a second can be followed.

In vivo detection of hepatitis C virus (HCV) RNA in the brain in a case of encephalitis: evidence for HCV neuroinvasion

2008 ◽  
Vol 15 (3) ◽  
pp. 214-218 ◽  
Author(s):  
F. Seifert ◽  
T. Struffert ◽  
M. Hildebrandt ◽  
I. Blümcke ◽  
W. Brück ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Rna ◽  
In Vivo Detection ◽  
The Brain

Effects of acute ethanol on opioid peptide release in the central amygdala: an in vivo microdialysis study

2008 ◽  
Vol 201 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Minh P. Lam ◽  
Peter W. Marinelli ◽  
Li Bai ◽  
Christina Gianoulakis
Keyword(s):  
Opioid Peptide ◽  
In Vivo Microdialysis ◽  
Central Amygdala ◽  
Peptide Release ◽  
Acute Ethanol ◽  
Microdialysis Study
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