scholarly journals Neurocalcin regulates nighttime sleep and arousal in Drosophila

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Ko-Fan Chen ◽  
Simon Lowe ◽  
Angélique Lamaze ◽  
Patrick Krätschmer ◽  
James Jepson
Keyword(s):  
Fruit Fly ◽  
Sleep Stages ◽  
Mushroom Bodies ◽  
Night Sleep ◽  
Temporal Window ◽  
Light Sensing ◽  
Synaptic Release ◽  
Sensor Protein ◽  
Neuronal Calcium Sensor Protein ◽  
Nighttime Sleep

Sleep-like states in diverse organisms can be separated into distinct stages, each with a characteristic arousal threshold. However, the molecular pathways underlying different sleep stages remain unclear. The fruit fly, Drosophila melanogaster, exhibits consolidated sleep during both day and night, with night sleep associated with higher arousal thresholds compared to day sleep. Here we identify a role for the neuronal calcium sensor protein Neurocalcin (NCA) in promoting sleep during the night but not the day by suppressing nocturnal arousal and hyperactivity. We show that both circadian and light-sensing pathways define the temporal window in which NCA promotes sleep. Furthermore, we find that NCA promotes sleep by suppressing synaptic release from a dispersed wake-promoting neural network and demonstrate that the mushroom bodies, a sleep-regulatory center, are a module within this network. Our results advance the understanding of how sleep stages are genetically defined.

scholarly journals A Convolutional Network for the Classification of Sleep Stages

Proceedings ◽  
2018 ◽  
Vol 2 (18) ◽  
pp. 1174 ◽  
Author(s):  
Isaac Fernández-Varela ◽  
Elena Hernández-Pereira ◽  
Vicente Moret-Bonillo
Keyword(s):  
Sleep Medicine ◽  
Sleep Stages ◽  
Kappa Index ◽  
Night Sleep ◽  
Convolutional Network ◽  
Sleep Study ◽  
Convolutional Networks ◽  
Sleep Studies ◽  
Automatic Methods

The classification of sleep stages is a crucial task in the context of sleep medicine. It involves the analysis of multiple signals thus being tedious and complex. Even for a trained physician scoring a whole night sleep study can take several hours. Most of the automatic methods trying to solve this problem use human engineered features biased for a specific dataset. In this work we use deep learning to avoid human bias. We propose an ensemble of 5 convolutional networks achieving a kappa index of 0.83 when classifying 500 sleep studies.

scholarly journals Gastric Acid Secretion and Sleep Stages During Natural Night Sleep

1975 ◽  
Vol 68 (6) ◽  
pp. 1449-1455 ◽  
Author(s):  
G. Stacher ◽  
B. Presslich ◽  
H. Stärker
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Sleep Stages ◽  
Night Sleep

scholarly journals Associations between parent-reported and objectively measured sleep duration and timing in infants at age 6 months

SLEEP ◽  
2020 ◽  
Author(s):  
Mirja Quante ◽  
Benjamin Hong ◽  
Tayla von Ash ◽  
Xinting Yu ◽  
Emily R Kaplan ◽  
...  
Keyword(s):  
Sleep Duration ◽  
Low Income ◽  
Sleep Onset ◽  
Sleep Diary ◽  
Study Cohort ◽  
Linear Regression Models ◽  
Night Sleep ◽  
Low Income Families ◽  
Objectively Measured ◽  
Nighttime Sleep

Abstract Study Objectives To compare the estimates of sleep duration and timing from survey, diary, and actigraphy in infants at age 6 months, overall and by select demographics and other factors. Methods In total, 314 infants participating in the Rise & SHINE (Sleep Health in Infancy & Early Childhood study) cohort in Boston, MA, USA, wore an actigraph on their left ankle for 7 days. Parents concurrently completed a sleep diary and the expanded version of the Brief Infant Sleep Questionnaire. Concordance between parent-reported and objective sleep estimates was assessed using Bland–Altman plots, Spearman’s rank correlations, intraclass correlations, and linear regression models. Results Mean infant age was 6.4 (0.6 SD) months; 51% were female and 42% were Non-Hispanic white. Mean total sleep duration using actigraphy was 526 (67 SD) minutes per night, 143 (42 SD) minutes per day, and 460 (100 SD) minutes during the longest nighttime sleep period. Relative to actigraphy, parent-completed survey and diary overestimated total day (by 29 and 31 minutes, respectively) and night sleep duration (67 and 43 minutes, respectively) and underestimated the longest sleep (58 minutes), with the highest agreement for sleep onset and offset timing (differences < 30 minutes). There was a tendency toward greater bias among short- and long-sleeping infants. Self-reporting bias for diary-measured longest nighttime sleep and total night sleep duration was higher in infants of parents reporting a problem with their baby’s night awakenings and in low-income families, respectively. Conclusions Our findings underscore the need to be cautious when comparing findings across studies using different sleep assessment methods.

scholarly journals Interaction between neuronal calcium sensor protein 1 and lithium in pedunculopontine neurons

2017 ◽  
Vol 5 (7) ◽  
pp. e13246 ◽  
Author(s):  
Stasia D'Onofrio ◽  
James Hyde ◽  
Edgar Garcia-Rill
Keyword(s):  
Calcium Sensor ◽  
Neuronal Calcium Sensor ◽  
Sensor Protein ◽  
Neuronal Calcium Sensor Protein

scholarly journals Neuronal Calcium Sensor Protein Visinin-like Protein-3 Interacts with Microsomal Cytochromeb5in a Ca2+-dependent Manner

2004 ◽  
Vol 279 (15) ◽  
pp. 15142-15152 ◽  
Author(s):  
Kensuke Oikawa ◽  
Shoji Kimura ◽  
Naoko Aoki ◽  
Yoshiaki Atsuta ◽  
Yumi Takiyama ◽  
...  
Keyword(s):  
Dependent Manner ◽  
Calcium Sensor ◽  
Neuronal Calcium Sensor ◽  
Sensor Protein ◽  
Neuronal Calcium Sensor Protein

Body movements during night sleep and their relationship with sleep stages are further modified in very old subjects

2008 ◽  
Vol 75 (1) ◽  
pp. 66-69 ◽  
Author(s):  
Fiorenza Giganti ◽  
Gianluca Ficca ◽  
Sara Gori ◽  
Piero Salzarulo
Keyword(s):  
Sleep Stages ◽  
Night Sleep ◽  
Body Movements ◽  
Very Old

FoxP expression identifies a Kenyon cell subtype in the honeybee mushroom bodies linking them to fruit fly αβcneurons

2017 ◽  
Vol 46 (9) ◽  
pp. 2534-2541 ◽  
Author(s):  
Adriana Schatton ◽  
Constance Scharff
Keyword(s):  
Fruit Fly ◽  
Mushroom Bodies ◽  
Kenyon Cell ◽  
Cell Subtype

scholarly journals Identification of genes required for apical protein trafficking in Drosophila photoreceptor cells

2019 ◽  
Author(s):  
Azadeh Laffafian ◽  
Ulrich Tepass
Keyword(s):  
Protein Trafficking ◽  
Apical Membrane ◽  
Vesicle Trafficking ◽  
Retinal Disease ◽  
Photoreceptor Cells ◽  
Fruit Fly ◽  
Light Sensing ◽  
Human Orthologs ◽  
Apical Trafficking ◽  
Drosophila Photoreceptor

AbstractDrosophila photoreceptor cells (PRCs) are highly polarized epithelial cells. Their apical membrane is further subdivided into the stalk membrane and the light-sensing rhabdomere. The photo-pigment Rhodopsin1 (Rh1) localizes to the rhabdomere, whereas the apical determinant Crumbs (Crb) is enriched at the stalk membrane. The proteoglycan Eyes shut (Eys) is secreted through the apical membrane into an inter-rhabdomeral space. Rh1, Crb, and Eys are essential for PRC development, normal vision, and PRC survival. Human orthologs of all three proteins have been linked to retinal degenerative diseases. Here, we describe an RNAi-based screen examining the importance of approximately 240 trafficking-related genes in apical trafficking of Eys, Rh1, and Crb. We found 28 genes that have an effect on the localization and/or levels of these apical proteins and analyzed several factors in more detail. We show that the Arf GEF protein Sec71 is required for biosynthetic traffic of both apical and basolateral proteins, that the exocyst complex and the microtubule-based motor proteins dynein and kinesin promote the secretion of Eys and Rh1, and that Syntaxin 7/Avalanche controls the endocytosis of Rh1, Eys, and Crb.Article summeryPhotoreceptor cells (PRCs) rely on polarized vesicle trafficking to deliver key secreted and transmembrane proteins to their correct locations. Failure to do so causes defects in PRC development, function, and survival leading to retinal disease. Using the fruit fly Drosophila as a model we have identified 28 genes that are required for the trafficking of the three apical proteins Rhodopsin 1, Crumbs, and Eyes Shut. Human homologs of all three genes are associated with retinal degeneration. We characterized several genes to reveal novel mechanisms of vesicle trafficking in photoreceptor cells at different points in the biosynthetic or endocytotic pathways.

Sign in / Sign up

Export Citation Format

Share Document