scholarly journals Decision letter: Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

2020 ◽  
Author(s):  
Yukiko M Yamashita ◽  
Jianjun Sun
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Germline Stem Cell

scholarly journals dSETDB1 and SU(VAR)3–9 Sequentially Function during Germline-Stem Cell Differentiation in Drosophila melanogaster

PLoS ONE ◽  
2008 ◽  
Vol 3 (5) ◽  
pp. e2234 ◽  
Author(s):  
Jeongheon Yoon ◽  
Kyu-Sun Lee ◽  
Jung Sun Park ◽  
Kweon Yu ◽  
Sang-Gi Paik ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Germline Stem Cell

scholarly journals Author response: Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

2020 ◽  
Author(s):  
Yuto Yoshinari ◽  
Tomotsune Ameku ◽  
Shu Kondo ◽  
Hiromu Tanimoto ◽  
Takayuki Kuraishi ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Author Response ◽  
Germline Stem Cell

scholarly journals Adipocyte Metabolic Pathways Regulated by Diet Control the Female Germline Stem Cell Lineage in Drosophila melanogaster

Genetics ◽  
2017 ◽  
Vol 206 (2) ◽  
pp. 953-971 ◽  
Author(s):  
Shinya Matsuoka ◽  
Alissa R. Armstrong ◽  
Leesa L. Sampson ◽  
Kaitlin M. Laws ◽  
Daniela Drummond-Barbosa
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Metabolic Pathways ◽  
Cell Lineage ◽  
Germline Stem Cell ◽  
Stem Cell Lineage ◽  
Diet Control ◽  
Female Germline

scholarly journals RanBPM regulates cell shape, arrangement, and capacity of the female germline stem cell niche in Drosophila melanogaster

2008 ◽  
Vol 182 (5) ◽  
pp. 963-977 ◽  
Author(s):  
David A. Dansereau ◽  
Paul Lasko
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Cultured Cells ◽  
Adherens Junction ◽  
Germline Stem Cell ◽  
Surface Receptors ◽  
Skeletal Protein ◽  
Abnormal Accumulation ◽  
Cell Niche ◽  
Germline Stem Cell Niche

Experiments in cultured cells with Ran-binding protein M (RanBPM) suggest that it links cell surface receptors and cell adhesion proteins. In this study, we undertake a genetic study of RanBPM function in the germline stem cell (GSC) niche of Drosophila melanogaster ovaries. We find that two RanBPM isoforms are produced from alternatively spliced transcripts, the longer of which is specifically enriched in the GSC niche, a cluster of somatic cells that physically anchors GSCs and expresses signals that maintain GSC fate. Loss of the long isoform from the niche causes defects in niche organization and cell size and increases the number of GSCs attached to the niche. In genetic mosaics for a null RanBPM allele, we find a strong bias for GSC attachment to mutant cap cells and observe abnormal accumulation of the adherens junction component Armadillo (β-catenin) and the membrane skeletal protein Hu-li tai shao in mutant terminal filament cells. These results implicate RanBPM in the regulation of niche capacity and adhesion.

scholarly journals The Bric-à-Brac transcription factors are necessary for formation of functional germline stem cell niches through control of dpp expression in the Drosophila melanogaster ovary

2019 ◽  
Author(s):  
Laurine Miscopein Saler ◽  
Mathieu Bartoletti ◽  
Virginie Hauser ◽  
Anne-Marie Pret ◽  
Laurent Theodore ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Transcription Factors ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Somatic Cells ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Larval Stages ◽  
Functional Germline

AbstractMany studies have focused on the mechanisms of stem cell maintenance via their interaction with a particular niche or microenvironment in adult tissues, but how formation of a functional niche is initiated, including how stem cells within a niche are established, is less well understood. Adult Drosophila melanogaster ovary Germline Stem Cell (GSC) niches are comprised of somatic cells forming a stack called a Terminal Filament (TF) and underlying Cap Cells (CCs) and Escort Cells (ECs), which are in direct contact with GSCs. In the adult, the Engrailed (En) transcription factor is specifically expressed in niche cells where it directly controls expression of the decapentaplegic gene (dpp) encoding a member of the Bone Morphogenetic Protein (BMP) family of secreted signaling molecules, which are key factors for GSC maintenance. In late third instar larval ovaries, in response to BMP signaling from newly-formed niches, adjacent primordial germ cells become GSCs. The bric-à-brac paralogs (bab1 and bab2) encode BTB/POZ-domain containing transcription factors, that are also expressed in developing GSCs niches where they are required for TF formation. Here, we demonstrate that Bab1 and Bab2 display redundant cell autonomous function for TF morphogenesis and we identify a new function for these genes in GSC establishment. Moreover, we show that Bab proteins control dpp expression in otherwise correctly specified CCs, independently of En and its paralog Invected (Inv). In fact, our results also indicate that en/inv function in larval stages are neither essential for TF formation, nor GSC establishment. Finally, when bab2 was overexpressed in ovarian somatic cells outside of the niche, where en/inv were not expressed, ectopic BMP signaling activation was induced in adjacent germ cells of adult ovaries, which formed GSC-like tumors. Together, these results indicate that Bab transcription factors are positive regulators of BMP signaling for acquisition of GSC status.

scholarly journals Notch signaling is required for survival of the germline stem cell lineage in testes of Drosophila melanogaster

2019 ◽  
Author(s):  
Chun L. Ng ◽  
Qian Yue ◽  
Schulz Cordula
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Notch Signaling ◽  
Transition Zone ◽  
Cell Lineage ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Stem Cell Lineage ◽  
Cyst Cells ◽  
Germline Cells

AbstractIn all metazoan species, sperm is produced from germline stem cells. These self-renew and produce daughter cells that amplify and differentiate dependent on interactions with somatic support cells. In the male gonad of Drosophila melanogaster, the germline and somatic cyst cells co-differentiate as cysts, an arrangement in which the germline is completely enclosed by cytoplasmic extensions from the cyst cells. Notch is a developmentally relevant receptor in a pathway requiring immediate proximity with the signal sending cell. Here, we show that Notch is expressed in the cyst cells of wild-type testes. Notch becomes activated in the transition zone, an apical area of the testes in which the cyst cells express stage-specific transcription factors and the enclosed germline finalizes transit-amplifying divisions. Reducing the ligand Delta from the germline cells via RNA-Interference or reducing the receptor Notch from the cyst cells via CRISPR resulted in cell death concomitant with loss of germline cells from the transition zone. This shows that Notch signaling is essential for the survival of the germline stem cell lineage.

scholarly journals The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster

2009 ◽  
Vol 185 (4) ◽  
pp. 613-627 ◽  
Author(s):  
Akos Szakmary ◽  
Mary Reedy ◽  
Hongying Qi ◽  
Haifan Lin
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Double Mutant ◽  
Terminal Region ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Stem Cell Maintenance ◽  
Male Germline ◽  
Cytoplasmic Structures ◽  
Cell Maintenance

Yb regulates the proliferation of both germline and somatic stem cells in the Drosophila melanogaster ovary by activating piwi and hh expression in niche cells. In this study, we show that Yb protein is localized as discrete cytoplasmic spots exclusively in the somatic cells of the ovary and testis. These spots, which are different from all known cytoplasmic structures in D. melanogaster, are evenly electron-dense spheres 1.5 µm in diameter (herein termed the Yb body). The Yb body is frequently associated with mitochondria and a less electron-dense sphere of similar size that appears to be RNA rich. There are one to two Yb bodies/cell, often located close to germline cells. The N-terminal region of Yb is required for hh expression in niche cells, whereas the C-terminal region is required for localization to Yb bodies. The entire Yb protein is necessary for piwi expression in niche cells. A double mutant of Yb and a novel locus show male germline loss, revealing a function for Yb in male germline stem cell maintenance.

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