scholarly journals Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
J Roman Arguello ◽  
Liliane Abuin ◽  
Jan Armida ◽  
Kaan Mika ◽  
Phing Chian Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase Ii ◽  
Sensory Neurons ◽  
Chromatin Accessibility ◽  
Olfactory Sensory Neurons ◽  
Ionotropic Receptor ◽  
Genetic Switch ◽  
Pou Domain ◽  
Guidance Molecule ◽  
Neuronal Guidance

Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selectedIonotropic receptor(Ir)-expressing olfactory sensory neurons inDrosophila. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. UniqueIrexpression is not consistently associated with differences in chromatin accessibility, but rather to distinct transcription factor profiles. Genes that are heterogeneously expressed across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.

scholarly journals Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

2020 ◽  
Author(s):  
J. Roman Arguello ◽  
Liliane Abuin ◽  
Jan Armida ◽  
Kaan Mika ◽  
Phing Chian Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase Ii ◽  
Molecular Profiling ◽  
Chromatin Accessibility ◽  
Ionotropic Receptor ◽  
Genetic Switch ◽  
Pou Domain ◽  
Neural Populations ◽  
Guidance Molecule ◽  
Neuronal Guidance

AbstractDetermining the molecular properties of neurons is essential to understand their development, function and evolution. We used Targeted DamID (TaDa) to characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic Receptor (IR)-expressing sensory neurons in the Drosophila antenna. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not linked to substantial differences in chromatin accessibility, but rather to distinct transcription factor profiles. Heterogeneously-expressed genes across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.

Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes

1992 ◽  
Vol 12 (7) ◽  
pp. 3247-3261
Author(s):  
S Murphy ◽  
J B Yoon ◽  
T Gerster ◽  
R G Roeder
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rna Polymerase Iii ◽  
Small Nuclear Rna ◽  
Sequence Element ◽  
Proximal Sequence Element ◽  
Pou Domain ◽  
Polymerase Iii ◽  
Nuclear Rna

The promoters of both RNA polymerase II- and RNA polymerase III-transcribed small nuclear RNA (snRNA) genes contain an essential and highly conserved proximal sequence element (PSE) approximately 55 bp upstream from the transcription start site. In addition, the upstream enhancers of all snRNA genes contain binding sites for octamer-binding transcription factors (Octs), and functional studies have indicated that the PSE and octamer elements work cooperatively. The present study has identified and characterized a novel transcription factor (designated PTF) which specifically binds to the PSE sequence of both RNA polymerase II- and RNA polymerase III-transcribed snRNA genes. PTF binding is markedly potentiated by Oct binding to an adjacent octamer site. This potentiation is effected by Oct-1, Oct-2, or the conserved POU domain of these factors. In agreement with these results and despite the independent binding of Octs to the promoter, PTF and Oct-1 enhance transcription from the 7SK promoter in an interdependent manner. Moreover, the POU domain of Oct-1 is sufficient for significant in vitro activity in the presence of PTF. These results suggest that essential activation domains reside in PTF and that the potentiation of PTF binding by Octs plays a key role in the function of octamer-containing snRNA gene enhancers.

scholarly journals Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.

1992 ◽  
Vol 12 (7) ◽  
pp. 3247-3261 ◽  
Author(s):  
S Murphy ◽  
J B Yoon ◽  
T Gerster ◽  
R G Roeder
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rna Polymerase Iii ◽  
Small Nuclear Rna ◽  
Sequence Element ◽  
Proximal Sequence Element ◽  
Pou Domain ◽  
Polymerase Iii ◽  
Nuclear Rna

The promoters of both RNA polymerase II- and RNA polymerase III-transcribed small nuclear RNA (snRNA) genes contain an essential and highly conserved proximal sequence element (PSE) approximately 55 bp upstream from the transcription start site. In addition, the upstream enhancers of all snRNA genes contain binding sites for octamer-binding transcription factors (Octs), and functional studies have indicated that the PSE and octamer elements work cooperatively. The present study has identified and characterized a novel transcription factor (designated PTF) which specifically binds to the PSE sequence of both RNA polymerase II- and RNA polymerase III-transcribed snRNA genes. PTF binding is markedly potentiated by Oct binding to an adjacent octamer site. This potentiation is effected by Oct-1, Oct-2, or the conserved POU domain of these factors. In agreement with these results and despite the independent binding of Octs to the promoter, PTF and Oct-1 enhance transcription from the 7SK promoter in an interdependent manner. Moreover, the POU domain of Oct-1 is sufficient for significant in vitro activity in the presence of PTF. These results suggest that essential activation domains reside in PTF and that the potentiation of PTF binding by Octs plays a key role in the function of octamer-containing snRNA gene enhancers.

Identification of genes regulated by transcription factor KLF7 in differentiating olfactory sensory neurons

Gene ◽  
2007 ◽  
Vol 388 (1-2) ◽  
pp. 34-42 ◽  
Author(s):  
Daisuke Kajimura ◽  
Cecilia Dragomir ◽  
Francesco Ramirez ◽  
Friedrich Laub
Keyword(s):  
Transcription Factor ◽  
Sensory Neurons ◽  
Olfactory Sensory Neurons
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