scholarly journals Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Chirine Toufaily ◽  
Jérôme Fortin ◽  
Carlos AI Alonso ◽  
Evelyne Lapointe ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Selective Advantage ◽  
Gonadotropin Releasing Hormone ◽  
Lh Surge ◽  
Releasing Hormone ◽  
Ovarian Follicle Development ◽  
Carboxy Terminal ◽  
Gonadotropin Releasing Hormone Receptor ◽  
G Protein Coupled ◽  
Homologous Desensitization

Gonadotropin-releasing hormone (GnRH) is the primary neuropeptide controlling reproduction in vertebrates. GnRH stimulates follicle-stimulating hormone (FSH) and luteinizing hormone (LH) synthesis via a G protein-coupled receptor, GnRHR, in the pituitary gland. In mammals, GnRHR lacks a C-terminal cytosolic tail (Ctail) and does not exhibit homologous desensitization. This might be an evolutionary adaptation that enables LH surge generation and ovulation. To test this idea, we fused the chicken GnRHR Ctail to the endogenous murine GnRHR in a transgenic model. The LH surge was blunted, but not blocked in these mice. In contrast, they showed reductions in FSH production, ovarian follicle development, and fertility. Addition of the Ctail altered the nature of agonist-induced calcium signaling required for normal FSH production. The loss of the GnRHR Ctail during mammalian evolution is unlikely to have conferred a selective advantage by enabling the LH surge. The adaptive significance of this specialization remains to be determined.

scholarly journals Addition of a carboxy terminal tail to the normally tailless gonadotropin-releasing hormone receptor impairs fertility in female mice

2021 ◽  
Author(s):  
Chirine Toufaily ◽  
Jerome Fortin ◽  
Carlos A.I. Alonso ◽  
Evelyne Lapointe ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Selective Advantage ◽  
Gonadotropin Releasing Hormone ◽  
Lh Surge ◽  
Releasing Hormone ◽  
Ovarian Follicle Development ◽  
Carboxy Terminal ◽  
Gonadotropin Releasing Hormone Receptor ◽  
G Protein Coupled ◽  
Homologous Desensitization

Gonadotropin-releasing hormone (GnRH) is the primary neuropeptide controlling reproduction in vertebrates. GnRH stimulates follicle-stimulating hormone (FSH) and luteinizing hormone (LH) synthesis via a G protein-coupled receptor, GnRHR, in the pituitary gland. In mammals, GnRHR lacks a C-terminal cytosolic tail (Ctail) and does not exhibit homologous desensitization. This might be an evolutionary adaptation that enables LH surge generation and ovulation. To test this idea, we fused the chicken GnRHR Ctail to the endogenous murine GnRHR in a transgenic model. The LH surge was blunted, but not blocked in these mice. In contrast, they showed reductions in FSH production, ovarian follicle development, and fertility. Addition of the Ctail altered the nature of agonist-induced calcium signaling required for normal FSH production. The loss of the GnRHR Ctail during mammalian evolution is unlikely to have conferred a selective advantage by enabling the LH surge. The adaptive significance of this specialization remains to be determined.

Selective cleavage of ErbB4 by G-protein-coupled Gonadotropin-Releasing Hormone Receptor in Cultured Hypothalamic Neurons

2012 ◽  
Vol 227 (6) ◽  
pp. 2492-2501 ◽  
Author(s):  
Sayomi Higa-Nakamine ◽  
Noriko Maeda ◽  
Seikichi Toku ◽  
Tomoko Yamamoto ◽  
Matee Yingyuenyong ◽  
...  
Keyword(s):  
G Protein ◽  
Hormone Receptor ◽  
Gonadotropin Releasing Hormone ◽  
Hypothalamic Neurons ◽  
Releasing Hormone ◽  
Gonadotropin Releasing Hormone Receptor ◽  
G Protein Coupled

The expression, regulation and signal transduction pathways of the mammalian gonadotropin-releasing hormone receptor

2000 ◽  
Vol 78 (12) ◽  
pp. 1029-1052 ◽  
Author(s):  
Kwai Wa Cheng ◽  
Peter CK Leung
Keyword(s):  
Signal Transduction ◽  
Transcriptional Regulation ◽  
Protein Kinase ◽  
Protein Kinases ◽  
G Protein ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Flanking Region ◽  
Gonadotropin Releasing Hormone Receptor ◽  
G Protein Coupled

Normal mammalian sexual maturation and reproductive functions require the integration and precise coordination of hormones at the hypothalamic, pituitary, and gonadal levels. Hypothalamic gonadotropin-releasing hormone (GnRH) is a key regulator in this system; after binding to its receptor (GnRHR), it stimulates de novo synthesis and release of gonadotropins in anterior pituitary gonadotropes. Since the isolation of the GnRHR cDNA, the expression of GnRHR mRNA has been detected not only in the pituitary, but also in extrapituitary tissues, including the ovary and placenta. It has been shown that change in GnRHR mRNA is one of the mechanisms for regulating the expression of the GnRHR. To help understand the molecular mechanism(s) involved in transcriptional regulation of the GnRHR gene, the 5' flanking region of the GnRHR gene has recently been isolated. Initial characterization studies have identified several DNA regions in the GnRHR 5' flanking region which are responsible for both basal expression and GnRH-mediated homologous regulation of this gene in pituitary cells. The mammalian GnRHR lacks a C-terminus and possesses a relatively short third intracellular loop; both features are important in desensitization of many others G-protein coupled receptors (GPCRs), Homologous desensitization of GnRHR has been shown to be regulated by various serine-threonine protein kinases including protein kinase A (PKA) and protein kinase C (PKC), as well as by G-protein coupled receptor kinases (GRKs). Furthermore, GnRHR was demonstrated to couple with multiple G proteins (Gq/11, Gs, and Gi), and to activate cascades that involved the PKC, PKA, and mitogen-activator protein kinases. These results suggest the diversity of GnRHR-G protein coupling and signal transduction systems. The identification of second form of GnRH (GnRH-II) in mammals adds to the complexity of the GnRH-GnRHR system. This review summaries our recent progress in understanding the regulation of GnRHR gene expression and the GnRHR signal transduction pathways.Key words: gonadotropin-releasing hormone receptor, transcriptional regulation, desensitization, signal transduction.

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