Isolation and Characterization of Actinomycete Strain BK185 Possessing Antifungal Activity against Ginseng Root Rot Pathogens

A new actinomycete strain was isolated from Western part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. The present paper describes the isolation and characterization of compound 1 from this new Streptomyces species with the help of various chemical and spectroscopic methods. Antimicrobial activity of compound 1 was tested by disc diffusion assay method and compared with that of standard antibiotics (Kanamycin for antibacterial activity and Nystatin for antifungal activity). The compound has been found to exhibit moderate to strong antimicrobial activity against the test organisms. Cytotoxicity of the compound 1 and the pet. ether extract of Czapek Dox (alkaline) broth of Streptomyces species was evaluated in brine shrimp bioassay with LC50 values of 23.85 µg/ml (ppm) and 19.95 µg/ml (ppm), respectively. Keywords: Streptomyces; Antimicrobial activity; Cytotoxicity. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.3079 J. Sci. Res. 2 (1), 178-185 (2010)

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Isolation and Characterization of an actinomycete strain producing an antifungal metabolite effective against Candida albicans

Research Journal of Pharmacy and Technology ◽

10.5958/0974-360x.2019.00791.1 ◽

2019 ◽

Vol 12 (10) ◽

pp. 4601

Author(s):

Adithya Chandrashekar ◽

Anuraag Muralidharan ◽

Ananthamurthy Koteshwara ◽

Angel Treasa Alex ◽

V. M. Subrahmanyam

Keyword(s):

Candida Albicans ◽

Antifungal Metabolite ◽

Isolation And Characterization ◽

Actinomycete Strain

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Isolation and characterization of metabolites from Bacillus licheniformis MH48 with antifungal activity against plant pathogens

Microbial Pathogenesis ◽

10.1016/j.micpath.2017.07.027 ◽

2017 ◽

Vol 110 ◽

pp. 645-653 ◽

Cited By ~ 16

Author(s):

Min-Hae Jeong ◽

Yong-Seong Lee ◽

Jeong-Yong Cho ◽

Young-Sang Ahn ◽

Jae-Hak Moon ◽

...

Keyword(s):

Antifungal Activity ◽

Bacillus Licheniformis ◽

Plant Pathogens ◽

Isolation And Characterization

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ISOLATION AND CHARACTERIZATION OF A NOVEL HYALURONIDASE INHIBITOR FROM A MARINE ACTINOMYCETES STRAIN

KnE Life Sciences ◽

10.18502/kls.v1i0.94 ◽

2015 ◽

Vol 2 (1) ◽

pp. 114 ◽

Cited By ~ 1

Author(s):

Enjuro Harunari ◽

Chiaki Imada ◽

Yasuhiro Igarashi ◽

Takao Fukuda ◽

Takeshi Terahara ◽

...

Keyword(s):

Inhibitory Activity ◽

Tokyo Bay ◽

Marine Actinomycetes ◽

Moderate Growth ◽

Isolation And Characterization ◽

Nmr Data ◽

Hyaluronidase Inhibitor ◽

Actinomycete Strain ◽

Culture Extract

A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine- derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin. Keywords: marine actinomycetes; Streptomyces; hyaluronidase inhibitor

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