scholarly journals ISOLATION AND CHARACTERIZATION OF A NOVEL HYALURONIDASE INHIBITOR FROM A MARINE ACTINOMYCETES STRAIN

2015 ◽  
Vol 2 (1) ◽  
pp. 114 ◽  
Author(s):  
Enjuro Harunari ◽  
Chiaki Imada ◽  
Yasuhiro Igarashi ◽  
Takao Fukuda ◽  
Takeshi Terahara ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Tokyo Bay ◽  
Marine Actinomycetes ◽  
Moderate Growth ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Hyaluronidase Inhibitor ◽  
Actinomycete Strain ◽  
Culture Extract

A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine- derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin. Keywords: marine actinomycetes; Streptomyces; hyaluronidase inhibitor 

Isolation and characterization of sesquiterpenes from Arecophila saccharicola YMJ96022401 with NO production inhibitory activity

2013 ◽  
Vol 85 ◽  
pp. 129-136 ◽  
Author(s):  
Lin-Wen Lee ◽  
Guei-Jane Wang ◽  
Mei-Hsiang Lin ◽  
Yu-Min Ju ◽  
Yen-Wen Lin ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
No Production ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Hypolipidemic Compound from Cajanus cajan

2011 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
M Anwar Habib ◽  
ASM Anisuzzaman ◽  
RK Barman ◽  
M Abdul Gafur ◽  
M Tofazzal Haque
Keyword(s):  
Cajanus Cajan ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypolipidemic Activity ◽  
1H And 13C Nmr ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Biological Studies ◽  
Isolated Compound

The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram). The methanol extract of redgram seeds was found to decrease fasting blood glucose and lipid profile (p<0.001) on streptozotocin-induced mice compared to control. This activity- guided fraction led to the isolation of a compound, substituted benzene containing polyhydroxy functions fused with lactone (CCA3) by analysis of 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA3 seems to be the first report on hypolipidemic activity from methanol extract of redgram. TAJ 2011; 24(1): 6-10

Screening, Isolation and Characterization of a Novel Antimicrobial Producing Actinomycete, Strain RAF10

2007 ◽  
Vol 6 (4) ◽  
pp. 489-496 ◽  
Author(s):  
Forar Laidi Rabah ◽  
Ali Elshafei ◽  
Mahmoud Saker ◽  
Bengraa Cheikh ◽  
Hacene Hocine
Keyword(s):  
Isolation And Characterization ◽  
Actinomycete Strain

scholarly journals Characterization and Antimicrobial Activities of a Metabolite from a New Streptomyces Species from Bangladeshi Soil

2009 ◽  
Vol 2 (1) ◽  
pp. 178-185
Author(s):  
M. Akhand ◽  
M. A. A. Al-Bari ◽  
M. A. Islam ◽  
Proma Khondkar
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Assay Method ◽  
Streptomyces Species ◽  
Isolation And Characterization ◽  
Test Organisms ◽  
Actinomycete Strain ◽  
Diffusion Assay ◽  
Strong Antimicrobial Activity

A new actinomycete strain was isolated from Western part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. The present paper describes the isolation and characterization of compound 1 from this new Streptomyces species with the help of various chemical and spectroscopic methods. Antimicrobial activity of compound 1 was tested by disc diffusion assay method and compared with that of standard antibiotics (Kanamycin for antibacterial activity and Nystatin for antifungal activity). The compound has been found to exhibit moderate to strong antimicrobial activity against the test organisms. Cytotoxicity of the compound 1 and the pet. ether extract of Czapek Dox (alkaline) broth of Streptomyces species was evaluated in brine shrimp bioassay with LC50 values of 23.85 µg/ml (ppm) and 19.95 µg/ml (ppm), respectively. Keywords: Streptomyces; Antimicrobial activity; Cytotoxicity. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.3079                  J. Sci. Res. 2 (1), 178-185 (2010)  

Isolation and Characterization of an actinomycete strain producing an antifungal metabolite effective against Candida albicans

2019 ◽  
Vol 12 (10) ◽  
pp. 4601
Author(s):  
Adithya Chandrashekar ◽  
Anuraag Muralidharan ◽  
Ananthamurthy Koteshwara ◽  
Angel Treasa Alex ◽  
V. M. Subrahmanyam
Keyword(s):  
Candida Albicans ◽  
Antifungal Metabolite ◽  
Isolation And Characterization ◽  
Actinomycete Strain

scholarly journals Isolation and characterization of antibacterial substances produced by marine actinomycetes in the presence of seawater

2007 ◽  
Vol 21 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Chiaki Imada ◽  
Naoko Koseki ◽  
Masazumi Kamata ◽  
Takeshi Kobayashi ◽  
Naoko Hamada-Sato
Keyword(s):  
Marine Actinomycetes ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of benzo[a]pyrene-degrading bacteria from the Tokyo Bay area and Tama River in Japan

2015 ◽  
Vol 362 (18) ◽  
pp. fnv143 ◽  
Author(s):  
Masahiko Okai ◽  
Ikumi Kihara ◽  
Yuto Yokoyama ◽  
Masami Ishida ◽  
Naoto Urano
Keyword(s):  
Tokyo Bay ◽  
Bay Area ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Preparation, isolation, and characterization of permethylated galactosides and fucosides

1999 ◽  
Vol 77 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Daniel D Asres ◽  
Hélène Perreault
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Characterization Methods ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Layer Chromatography ◽  
Structural Aspects

Sugar analysis involving permethylation followed by methanolysis can lead to significant results, given that permethylated monosaccharide standards are available for comparison of spectral and chromatographic data. Such standards are not readily commercially available, especially the furanoside forms. This note describes the isolation and characterization of permethylated pyranoside and furanoside species of D(+)-galactose and L(-)-fucose. Separation of the isomers was optimized using a combination of column chromatography and continuous elution thin-layer chromatography (TLC). TLC, gas chromatography - mass spectrometry, and 1H nuclear magnetic resonance (NMR) spectroscopy were used as the characterization methods. The isolation of furanosides is emphasized, since no specific NMR data have been reported on those to date, while several reports have already discussed the structural aspects of pyranosides.Key words: permethylation, monosaccharide, GC-MS, TLC, NMR.

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