scholarly journals The influence of storage time on ponazuril concentrations in feline plasma

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12516
Author(s):  
Sherry Cox ◽  
Lainey Harvill ◽  
Sarah Singleton ◽  
Joan B. Bergman ◽  
Becky DeBolt
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Storage Time ◽  
Plasma Samples ◽  
Ultraviolet Detection ◽  
Minimal Loss ◽  
Spiked Plasma ◽  
Average Loss ◽  
The Stability

Background The pharmacokinetics of ponazuril have been determined in several species; however, there is very little information on the stability of the drug after storage for long periods of time. This study was undertaken to determine the stability of ponazuril in plasma samples stored at −80 °C, which is the temperature most commonly used in the author’s laboratory. Method Spiked plasma samples (0.3, 7.5, and 15 µg/mL) were stored at −80 °C for three months. Analysis occurred on the first day and then once a week for the following twelve weeks. The drug was extracted using a chloroform extraction and separated by high performance liquid chromatography using ultraviolet detection. Results There was no loss of drug for any concentration for the first four weeks of storage. There was an average loss of less than 5% from day 35 through day 70 and an average loss of 6% on day 77 and 84. The data suggest that ponazuril is stable for 4 weeks when stored at −80 °C and undergoes minimal loss in the remaining 8 weeks.

scholarly journals Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 959
Author(s):  
Ana Alarcia-Lacalle ◽  
Helena Barrasa ◽  
Javier Maynar ◽  
Andrés Canut-Blasco ◽  
Carmen Gómez-González ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pharmacokinetic Profile ◽  
Acquisition Time ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Short Acquisition Time ◽  
Ultraviolet Detection ◽  
Reproducible Method

This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, the supernatant was injected into the HPLC system, working in isocratic mode. Ceftaroline was detected at 238 nm at a short acquisition time (less than 5 min). The calibration curve was linear over the concentration range from 0.25 to 40 µg/mL, and the method appeared to be selective, precise and accurate. Ceftaroline in plasma samples was stable at −80 °C for at least 3months. The method was successfully applied to characterize the pharmacokinetic profile of ceftaroline in two critically ill patients and to evaluate whether the pharmacokinetic/pharmacodynamic (PK/PD) target was reached or not with the dose regimen administered.

Determination of fexofenadine in Hank's balanced salt solution by high-performance liquid chromatography with ultraviolet detection: application to Caco-2 cell permeability studies

2014 ◽  
Vol 29 (4) ◽  
pp. 537-544 ◽  
Author(s):  
Marco Antônio dos. Reis Júnior ◽  
Ana Cláudia Miranda de Faria ◽  
Eudes da. Silva Velozo ◽  
Teresa Dalla Costa ◽  
Frank Pereira de Andrade ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Salt Solution ◽  
High Performance ◽  
Cell Permeability ◽  
Ultraviolet Detection ◽  
Permeability Studies
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