scholarly journals The relationship between carbon and nitrogen metabolism in cucumber leaves acclimated to salt stress

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6043 ◽  
Author(s):  
Marcin Robert Naliwajski ◽  
Maria Skłodowska
Keyword(s):  
Salt Stress ◽  
Nitrogen Metabolism ◽  
Tca Cycle ◽  
Nacl Stress ◽  
G6pdh Activity ◽  
High Demand ◽  
Carbon And Nitrogen ◽  
Adaptive Processes ◽  
Carbon And Nitrogen Metabolism ◽  
The Relationship

The study examines the effect of acclimation on carbon and nitrogen metabolism in cucumber leaves subjected to moderate and severe NaCl stress. The levels of glucose, sucrose, NADH/NAD+-GDH, AspAT, AlaAT, NADP+-ICDH, G6PDH and 6GPDH activity were determined after 24 and 72 hour periods of salt stress in acclimated and non-acclimated plants. Although both groups of plants showed high Glc and Suc accumulation, they differed with regard to the range and time of accumulation. Acclimation to salinity decreased the activities of NADP+-ICDH and deaminating NAD+-GDH compared to controls; however, these enzymes, together with the other examined parameters, showed elevated values in the stressed plants. The acclimated plants showed higher G6PDH activity than the non-acclimated plants, whereas both groups demonstrated similar 6PGDH activity. The high activities of NADH-GDH, AlaAT and AspAT observed in the examined plants could be attributed to a high demand for glutamate. The observed changes may be required for the maintenance of correct TCA cycle activity, and acclimation appeared to positively influence these adaptive processes.

scholarly journals Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

2020 ◽  
Vol 21 (3) ◽  
pp. 774
Author(s):  
Xuemei Xiao ◽  
Jian Lv ◽  
Jianming Xie ◽  
Zhi Feng ◽  
Ning Ma ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Expression Patterns ◽  
Calvin Cycle ◽  
Biomass Accumulation ◽  
Tca Cycle ◽  
Mapk Signaling ◽  
Carbon And Nitrogen ◽  
Related Transcription Factor ◽  
Carbon And Nitrogen Metabolism

Autotoxicity of root exudates is one of the main reasons for consecutive monoculture problem (CMP) in cucumber under greenhouse cultivation. Rootstock grafting may improve the tolerance of cucumber plants to autotoxic stress. To verify the enhanced tolerance to autotoxic stress and illuminate relevant molecular mechanism, a transcriptomic comparative analysis was performed between rootstock grafted (RG) and non-grafted (NG) cucumber plants by a simulation of exogenous cinnamic acid (CA). The present study confirmed that relatively stable plant growth, biomass accumulation, chlorophyll content, and photosynthesis was observed in RG than NG under CA stress. We identified 3647 and 2691 differentially expressed genes (DEGs) in NG and RG cucumber plants when compared to respective control, and gene expression patterns of RNA-seq was confirmed by qRT-PCR. Functional annotations revealed that DEGs response to CA stress were enriched in pathways of plant hormone signal transduction, MAPK signaling pathway, phenylalanine metabolism, and plant-pathogen interaction. Interestingly, the significantly enriched pathway of photosynthesis-related, carbon and nitrogen metabolism only identified in NG, and most of DEGs were down-regulated. However, most of photosynthesis, Calvin cycle, glycolysis, TCA cycle, and nitrogen metabolism-related DEGs exhibited not or slightly down-regulated in RG. In addition, several stress-related transcription factor families of AP2/ERF, bHLH, bZIP, MYB. and NAC were uniquely triggered in the grafted cucumbers. Overall, the results of this study suggest that rootstock grafting improve the tolerance of cucumber plants to autotoxic stress by mediating down-regulation of photosynthesis, carbon, and nitrogen metabolism-related DEGs and activating the function of stress-related transcription factor. The transcriptome dataset provides an extensive sequence resource for further studies of autotoxic mechanism at molecular level.

Response of Carbon and Nitrogen Metabolism and Secondary Metabolites to Drought Stress and Salt Stress in Plants

2019 ◽  
Vol 62 (6) ◽  
pp. 387-399 ◽  
Author(s):  
Gaochang Cui ◽  
Yu Zhang ◽  
Wenjin Zhang ◽  
Duoyong Lang ◽  
Xiaojia Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Secondary Metabolites ◽  
Nitrogen Metabolism ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism

scholarly journals An Integrated Analysis of the Rice Transcriptome and Metabolome Reveals Differential Regulation of Carbon and Nitrogen Metabolism in Response to Nitrogen Availability

2019 ◽  
Vol 20 (9) ◽  
pp. 2349 ◽  
Author(s):  
Wei Xin ◽  
Lina Zhang ◽  
Wenzhong Zhang ◽  
Jiping Gao ◽  
Jun Yi ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Availability ◽  
Tca Cycle ◽  
Integrated Analysis ◽  
Nitrogen Use ◽  
Carbon And Nitrogen ◽  
Use Efficiency ◽  
Carbon And Nitrogen Metabolism ◽  
Low Nitrogen

Nitrogen (N) is an extremely important macronutrient for plant growth and development. It is the main limiting factor in most agricultural production. However, it is well known that the nitrogen use efficiency (NUE) of rice gradually decreases with the increase of the nitrogen application rate. In order to clarify the underlying metabolic and molecular mechanisms of this phenomenon, we performed an integrated analysis of the rice transcriptome and metabolome. Both differentially expressed genes (DEGs) and metabolite Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that carbon and nitrogen metabolism is significantly affected by nitrogen availability. Further analysis of carbon and nitrogen metabolism changes in rice under different nitrogen availability showed that high N inhibits nitrogen assimilation and aromatic metabolism pathways by regulating carbon metabolism pathways such as the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway (PPP). Under low nitrogen, the TCA cycle is promoted to produce more energy and α-ketoglutarate, thereby enhancing nitrogen transport and assimilation. PPP is also inhibited by low N, which may be consistent with the lower NADPH demand under low nitrogen. Additionally, we performed a co-expression network analysis of genes and metabolites related to carbon and nitrogen metabolism. In total, 15 genes were identified as hub genes. In summary, this study reveals the influence of nitrogen levels on the regulation mechanisms for carbon and nitrogen metabolism in rice and provides new insights into coordinating carbon and nitrogen metabolism and improving nitrogen use efficiency in rice.

Sign in / Sign up

Export Citation Format

Share Document