Bortezomib Modulates Regulatory T Cell Subpopulations in the Process of Acute Graft-Versus-Host Disease

2013 ◽  
Vol 59 (01+02/2013) ◽  
Author(s):  
Jianyu Weng ◽  
Peilong Lai ◽  
Meikun Lv ◽  
Shaoze Lin ◽  
Wei Ling ◽  
...  
Keyword(s):  
T Cell ◽  
Graft Versus Host Disease ◽  
Regulatory T Cell ◽  
Host Disease ◽  
Graft Versus Host ◽  
Cell Subpopulations ◽  
T Cell Subpopulations ◽  
Acute Graft

scholarly journals CD155 Regulates Regulatory T Cell Population and Attenuates Acute Graft-Versus-Host Disease

2015 ◽  
Vol 21 (2) ◽  
pp. S335
Author(s):  
Minoru Kanaya ◽  
Kazuko Shibuya ◽  
Fumie Abe ◽  
Takanori Teshima ◽  
Akira Shibuya
Keyword(s):  
T Cell ◽  
Cell Population ◽  
Graft Versus Host Disease ◽  
Regulatory T Cell ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

scholarly journals In vivo T-cell clonal amplification at time of acute graft-versus-host disease

Blood ◽  
1994 ◽  
Vol 84 (8) ◽  
pp. 2815-2820 ◽  
Author(s):  
PY Dietrich ◽  
A Caignard ◽  
A Lim ◽  
V Chung ◽  
JL Pico ◽  
...  
Keyword(s):  
T Cell ◽  
Graft Versus Host Disease ◽  
Donor Blood ◽  
Host Disease ◽  
Graft Versus Host ◽  
Junctional Region ◽  
Run Off ◽  
Pcr Products ◽  
Acute Graft

In a series of patients transplanted with HLA-matched allogeneic bone marrow grafts (alloBMT), we previously showed that a few T-cell receptor (TCR) V alpha and V beta gene segment transcripts were overexpressed in skin compared with blood at the time of acute graft- versus-host disease (aGVHD). Here, in one selected patient with overexpressed V beta 16 and V alpha 11 transcripts in skin, we analyzed the junctional variability of these transcripts in donor blood, patient blood, and skin collected at aGVHD onset. A unique junctional region sequence accounted for 81% of in frame V beta 16 transcripts (13 of 16) in skin and 59% (13 of 22) in patient blood. Similarly, two recurrent junctional region sequences were found in skin V alpha 11 transcripts, one accounting for 66% (21 of 32) and the other for 16% (5 of 32). These recurrences were also found in patient blood (36% and 15% of V alpha 11 transcripts, respectively). To extend our analysis, a polymerase chain reaction (PCR)-based method was used to precisely determine TCR beta transcript length in run-off reactions using uncloned bulk cDNA samples. All V beta-C beta PCR products analyzed in donor blood, as well as the majority of those analyzed in patient blood, included transcripts with highly diverse junctional region sizes. As expected from the sequence data, most V beta 16-C beta PCR products in skin and patient blood were of the same size (ie, same junctional region). In addition, V beta 3, V beta 5, and V beta 17 transcripts in skin were shown to display highly restricted size variability. The clonality of the V beta 16-C beta and V beta 17-C beta transcripts was further supported by the results of run-off reactions using 13 J beta specific primers. We have identified several recurrent TCR transcripts in skin, some of them also present in patient blood. These data support the view that several T-cell subpopulations are clonally expanded in vivo at the time of aGVHD onset in this case of related HLA-matched alloBMT.

scholarly journals A Crucial Role for Host APCs in the Induction of Donor CD4+CD25+ Regulatory T Cell-Mediated Suppression of Experimental Graft-versus-Host Disease

2010 ◽  
Vol 185 (7) ◽  
pp. 3866-3872 ◽  
Author(s):  
Isao Tawara ◽  
Warren D. Shlomchik ◽  
Angela Jones ◽  
Weiping Zou ◽  
Evelyn Nieves ◽  
...  
Keyword(s):  
T Cell ◽  
Graft Versus Host Disease ◽  
Regulatory T Cell ◽  
Crucial Role ◽  
Host Disease ◽  
Graft Versus Host

scholarly journals Graft-versus-host disease reduces regulatory T-cell migration into the tumour tissue

Immunology ◽  
2012 ◽  
Vol 137 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Christoph Dürr ◽  
Marie Follo ◽  
Marco Idzko ◽  
Wilfried Reichardt ◽  
Robert Zeiser
Keyword(s):  
Cell Migration ◽  
T Cell ◽  
Graft Versus Host Disease ◽  
Regulatory T Cell ◽  
Tumour Tissue ◽  
Host Disease ◽  
Graft Versus Host ◽  
T Cell Migration

scholarly journals In vivo T-cell clonal amplification at time of acute graft-versus-host disease

Blood ◽  
1994 ◽  
Vol 84 (8) ◽  
pp. 2815-2820 ◽  
Author(s):  
PY Dietrich ◽  
A Caignard ◽  
A Lim ◽  
V Chung ◽  
JL Pico ◽  
...  
Keyword(s):  
T Cell ◽  
Graft Versus Host Disease ◽  
Donor Blood ◽  
Host Disease ◽  
Graft Versus Host ◽  
Junctional Region ◽  
Run Off ◽  
Pcr Products ◽  
Acute Graft

Abstract In a series of patients transplanted with HLA-matched allogeneic bone marrow grafts (alloBMT), we previously showed that a few T-cell receptor (TCR) V alpha and V beta gene segment transcripts were overexpressed in skin compared with blood at the time of acute graft- versus-host disease (aGVHD). Here, in one selected patient with overexpressed V beta 16 and V alpha 11 transcripts in skin, we analyzed the junctional variability of these transcripts in donor blood, patient blood, and skin collected at aGVHD onset. A unique junctional region sequence accounted for 81% of in frame V beta 16 transcripts (13 of 16) in skin and 59% (13 of 22) in patient blood. Similarly, two recurrent junctional region sequences were found in skin V alpha 11 transcripts, one accounting for 66% (21 of 32) and the other for 16% (5 of 32). These recurrences were also found in patient blood (36% and 15% of V alpha 11 transcripts, respectively). To extend our analysis, a polymerase chain reaction (PCR)-based method was used to precisely determine TCR beta transcript length in run-off reactions using uncloned bulk cDNA samples. All V beta-C beta PCR products analyzed in donor blood, as well as the majority of those analyzed in patient blood, included transcripts with highly diverse junctional region sizes. As expected from the sequence data, most V beta 16-C beta PCR products in skin and patient blood were of the same size (ie, same junctional region). In addition, V beta 3, V beta 5, and V beta 17 transcripts in skin were shown to display highly restricted size variability. The clonality of the V beta 16-C beta and V beta 17-C beta transcripts was further supported by the results of run-off reactions using 13 J beta specific primers. We have identified several recurrent TCR transcripts in skin, some of them also present in patient blood. These data support the view that several T-cell subpopulations are clonally expanded in vivo at the time of aGVHD onset in this case of related HLA-matched alloBMT.

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