scholarly journals Diagnostic Pitfalls of Digital Microscopy Versus Light Microscopy in Gastrointestinal Pathology: A Systematic Review

Cureus ◽  
2021 ◽  
Author(s):  
Wangpan Shi ◽  
Petros Georgiou ◽  
Aqsa Akram ◽  
Matthew C Proute ◽  
Tatsiana Serhiyenia ◽  
...  
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Digital Microscopy ◽  
Gastrointestinal Pathology ◽  
Diagnostic Pitfalls

scholarly journals Clinical spectrum and diagnostic pitfalls of neurologic syndromes with Ri antibodies

2020 ◽  
Vol 7 (3) ◽  
pp. e699 ◽  
Author(s):  
Claire Simard ◽  
Alberto Vogrig ◽  
Bastien Joubert ◽  
Sergio Muñiz-Castrillo ◽  
Géraldine Picard ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Information ◽  
Review Of The Literature ◽  
Cerebellar Syndrome ◽  
Neurologic Disorder ◽  
Paraneoplastic Neurologic Syndrome ◽  
Clinical Difference ◽  
Oculomotor Disturbances ◽  
Diagnostic Pitfalls ◽  
French Cohort

ObjectiveTo describe the main syndrome and clinical course in a large cohort of patients with anti–Ri-associated paraneoplastic neurologic syndrome (Ri-PNS).MethodsTwenty-year retrospective nationwide study and systematic review of the literature.ResultsThirty-six patients with complete clinical information were identified (median age 66 years, range: 47–87 years). In this French cohort, the majority were women (78%). At onset, 4 main patterns were observed: cerebellar syndrome (39%), isolated tremor (24%), oculomotor disturbances (17%), and other symptoms (19%). Course was multistep for 78% of cases. At the time the disease reached the plateau phase (median 12 weeks, range: 1–64 weeks; 28% >3 months), 24 (67%) showed an overt cerebellar syndrome, which was isolated in 3 patients, and was most frequently (21/24 cases) part of a multisystem neurologic disease. Patients manifested a variety of movement disorders, including myoclonus (33%), dystonia (17%), either cervical or oromandibular, and parkinsonism (17%). Most patients had cancer (92%), mainly breast cancer (n = 22). Misdiagnoses concerned 22% of patients (n = 8) and included atypical parkinsonism (n = 2), MS (n = 2), Bickerstaff encephalitis (n = 1), hyperekplexia (n = 1), vestibular neuritis (n = 1), and functional neurologic disorder (n = 1). Survival at 12 months was 73% (95% CI [0.54–0.85]), at 24 months 62% (95% CI [0.41–0.78]), and at 36 months 47% (95% CI [0.25–0.65]). There was no major clinical difference between cases retrieved from the systematic review of the literature (n = 55) and the French cohort.ConclusionsRi-PNS is a multisystem neurologic syndrome with prominent cerebellum/brainstem involvement. Opsoclonus-myoclonus is less common than expected, and the disorder can mimic neurodegenerative diseases.

scholarly journals The Diagnostic Concordance of Whole Slide Imaging and Light Microscopy: A Systematic Review

2016 ◽  
Vol 141 (1) ◽  
pp. 151-161 ◽  
Author(s):  
Edward Goacher ◽  
Rebecca Randell ◽  
Bethany Williams ◽  
Darren Treanor
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Primary Diagnosis ◽  
Cochrane Library ◽  
Whole Slide Imaging ◽  
Tissue Samples ◽  
The Mean ◽  
Diagnostic Concordance ◽  
High Level ◽  
The Cochrane Library

Context.—Light microscopy (LM) is considered the reference standard for diagnosis in pathology. Whole slide imaging (WSI) generates digital images of cellular and tissue samples and offers multiple advantages compared with LM. Currently, WSI is not widely used for primary diagnosis. The lack of evidence regarding concordance between diagnoses rendered by WSI and LM is a significant barrier to both regulatory approval and uptake. Objective.—To examine the published literature on the concordance of pathologic diagnoses rendered by WSI compared with those rendered by LM. Data Sources.—We conducted a systematic review of studies assessing the concordance of pathologic diagnoses rendered by WSI and LM. Studies were identified following a systematic search of Medline (Medline Industries, Mundelein, Illinois), Medline in progress (Medline Industries), EMBASE (Elsevier, Amsterdam, the Netherlands), and the Cochrane Library (Wiley, London, England), between 1999 and March 2015. Conclusions.—Thirty-eight studies were included in the review. The mean diagnostic concordance of WSI and LM, weighted by the number of cases per study, was 92.4%. The weighted mean κ coefficient between WSI and LM was 0.75, signifying substantial agreement. Of the 30 studies quoting percentage concordance, 18 (60%) showed a concordance of 90% or greater, of which 10 (33%) showed a concordance of 95% or greater. This review found evidence to support a high level of diagnostic concordance. However, there were few studies, many were small, and they varied in quality, suggesting that further validation studies are still needed.

The performance of digital microscopy for primary diagnosis in human pathology: a systematic review

2019 ◽  
Vol 474 (3) ◽  
pp. 269-287 ◽  
Author(s):  
Anna Luíza Damaceno Araújo ◽  
Lady Paola Aristizábal Arboleda ◽  
Natalia Rangel Palmier ◽  
Jéssica Montenegro Fonsêca ◽  
Mariana de Pauli Paglioni ◽  
...  
Keyword(s):  
Systematic Review ◽  
Primary Diagnosis ◽  
Digital Microscopy ◽  
Human Pathology

scholarly journals EP.FRI.752 Delay to Surgery in Acute Perforated and Ischaemic Gastrointestinal Pathology – A Systematic Review

2021 ◽  
Vol 108 (Supplement_7) ◽  
Author(s):  
Victoria Murray ◽  
Joshua Burke ◽  
Michael Hughes ◽  
Claire Schofield ◽  
Alistair Young
Keyword(s):  
Systematic Review ◽  
Symptom Onset ◽  
Final Analysis ◽  
Mortality Prediction ◽  
Emergency Laparotomy ◽  
Management Framework ◽  
Gastrointestinal Pathology ◽  
National Mortality ◽  
Increased Risk ◽  
The Impact

Abstract Aims Patients with acute abdominal pathology requiring emergency laparotomy who experience a delay to theatre have an increased risk of morbidity, mortality and complications. The timeline between symptom onset and operation is ill-defined with international variance in assessment and management.  This systematic review aims to define where delays to surgery occur and assess the evidence for previous interventions. Methods A systematic review was performed searching MEDLINE and EMBASE databases (January 1st 2005 to May 6th 2020). All studies assessing the impact of time to theatre in patients with acute abdominal pathology requiring emergency laparotomy were considered. Results 17 studies were included in the final analysis.  15 unique timepoints were identified in the patient pathway between symptom onset and operation which could be classified into four distinct phases.   Time from admission to theatre (1 to 72 hours), and mortality rate (10.6-74.5%) varied greatly between studies.  Mean time to surgery was significantly higher in deceased patients compared to survivors.  Delays were related to imaging, diagnosis, decision-making, theatre availability and staffing.  Four of five interventional studies showed a reduced mortality following introduction of an acute laparotomy pathway.  Conclusions There is wide variation in the definition and measurement of time delays prior to emergency surgery with few studies exploring interventions.  Given the heterogenous nature of the patient population and pathologies, an assessment and management framework from onset of symptoms to operation is proposed.   This could be incorporated into national mortality prediction and audit tools and assist in the assessment of interventions.

scholarly journals Validation of Digital Microscopy Compared With Light Microscopy for the Diagnosis of Canine Cutaneous Tumors

2018 ◽  
Vol 55 (4) ◽  
pp. 490-500 ◽  
Author(s):  
Christof A. Bertram ◽  
Corinne Gurtner ◽  
Martina Dettwiler ◽  
Olivia Kershaw ◽  
Kristina Dietert ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Diagnostic Performance ◽  
Cell Tumor ◽  
Digital Microscopy ◽  
Round Cell ◽  
Round Cell Tumor ◽  
Glass Slides ◽  
Diagnostic Time ◽  
Tumor Types ◽  
Whole Slide Images

Integration of new technologies, such as digital microscopy, into a highly standardized laboratory routine requires the validation of its performance in terms of reliability, specificity, and sensitivity. However, a validation study of digital microscopy is currently lacking in veterinary pathology. The aim of the current study was to validate the usability of digital microscopy in terms of diagnostic accuracy, speed, and confidence for diagnosing and differentiating common canine cutaneous tumor types and to compare it to classical light microscopy. Therefore, 80 histologic sections including 17 different skin tumor types were examined twice as glass slides and twice as digital whole-slide images by 6 pathologists with different levels of experience at 4 time points. Comparison of both methods found digital microscopy to be noninferior for differentiating individual tumor types within the category epithelial and mesenchymal tumors, but diagnostic concordance was slightly lower for differentiating individual round cell tumor types by digital microscopy. In addition, digital microscopy was associated with significantly shorter diagnostic time, but diagnostic confidence was lower and technical quality was considered inferior for whole-slide images compared with glass slides. Of note, diagnostic performance for whole-slide images scanned at 200× magnification was noninferior in diagnostic performance for slides scanned at 400×. In conclusion, digital microscopy differs only minimally from light microscopy in few aspects of diagnostic performance and overall appears adequate for the diagnosis of individual canine cutaneous tumors with minor limitations for differentiating individual round cell tumor types and grading of mast cell tumors.

Categorising characteristics of counterfeit Marlboro cigarettes: a systematic review of tobacco industry documents

2019 ◽  
pp. tobaccocontrol-2018-054743
Author(s):  
Marin K Kurti ◽  
Klaus von Lampe ◽  
Yi He ◽  
Hiba Khanzada ◽  
Konstantina Kostara ◽  
...  
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Tobacco Industry ◽  
Forensic Analysis ◽  
Physical Characteristics ◽  
Systematic Analysis ◽  
Main Method ◽  
Cigarette Pack

BackgroundThere is scant research on methods used to identify counterfeit Marlboro cigarettes.MethodsSystematic analysis of internal tobacco industry documents characteristics of counterfeit Marlboro cigarettes.ResultsIn the industry documents we identified as relevant, there were 42 characteristics of counterfeit Marlboro cigarettes. Overall, physical characteristics (88.1%) were the most commonly identified features across all locations, with the pack blank, cardboard shell of the cigarette pack, as the dominant site (30.9%). Some of the physical characteristics included offset lithography printing, incorrect font and colour. Overall, light microscopy was identified as the main method of forensic analysis for detecting counterfeits.ConclusionIndependent researchers employing litter pack and pack swap surveys are encouraged to use the characteristics identified in the study to gauge the prevalence of counterfeit Marlboro cigarettes and compare against industry estimates.

scholarly journals Diagnostic concordance between whole slide imaging and conventional light microscopy in cytopathology: A systematic review

2019 ◽  
Vol 128 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Ilaria Girolami ◽  
Liron Pantanowitz ◽  
Stefano Marletta ◽  
Matteo Brunelli ◽  
Claudia Mescoli ◽  
...  
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Whole Slide Imaging ◽  
Diagnostic Concordance ◽  
Conventional Light Microscopy

scholarly journals The impact of emerging Plasmodium knowlesi on accurate diagnosis by light microscopy: a systematic review and modelling analysis

2021 ◽  
Author(s):  
John H. Huber ◽  
Margaret Elliott ◽  
Cristian Koepfli ◽  
T. Alex Perkins
Keyword(s):  
Systematic Review ◽  
Light Microscopy ◽  
Latent Class ◽  
Plasmodium Knowlesi ◽  
Meta Analysis ◽  
Latent Class Model ◽  
Malaria Diagnosis ◽  
Bayesian Hierarchical ◽  
Highly Correlated ◽  
The Impact

ABSTRACTBackgroundThe five Plasmodium spp. that cause human malaria appear similar under light microscopy, which raises the possibility that misdiagnosis could routinely occur in clinical settings. Assessing the extent of misdiagnosis is of particular importance for monitoring P. knowlesi, which co-circulates with the other Plasmodium spp.MethodsWe performed a systematic review and meta-analysis of studies comparing the performance of microscopy and PCR for diagnosing malaria in settings with co-circulation of the five Plasmodium spp. We assessed the extent to which co-circulation of Plasmodium parasites affects diagnostic outcomes. We fit a Bayesian hierarchical latent class model to estimate variation in microscopy sensitivity and specificity.ResultsMean sensitivity of microscopy was low, yet highly variable across Plasmodium spp., ranging from 41.7% (95% CI: 22.8 – 64.1%) for P. falciparum and 40.3% (22.0 – 61.5%) for P. vivax to 0.119% (0.0121 – 0.640%) for P. knowlesi, 7.57% (2.66 – 22.0%) for P. malariae, and 0.180% (0.00491 – 1.21%) for P. ovale. Observed PCR prevalence was positively correlated with estimated microscopic sensitivity and negatively correlated with estimated microscopic specificity, though the strength of the associations varied by species.ConclusionsOur analysis suggests that co-circulation of Plasmodium spp. undermines the accuracy of microscopy. Sensitivity was considerably lower for P. knowlesi, P. malariae, and P. ovale. The negative association between specificity and PCR prevalence imply that less frequently encountered species may be misdiagnosed as more frequently encountered species. Together, these results suggest that the burden of P. knowlesi, P. malariae, and P. ovale may be underappreciated in a clinical setting.SummaryCo-circulation of Plasmodium species parasites may limit the accuracy of malaria diagnosis by light microscopy. Variation in performance of light microscopy is highly correlated with the prevalence of the parasites in the epidemiological setting.

The use of digital microscopy as a teaching method for human pathology: a systematic review

2020 ◽  
Vol 477 (4) ◽  
pp. 475-486
Author(s):  
Carla Isabelly Rodrigues-Fernandes ◽  
Paul M. Speight ◽  
Syed Ali Khurram ◽  
Anna Luíza Damaceno Araújo ◽  
Danyel Elias da Cruz Perez ◽  
...  
Keyword(s):  
Systematic Review ◽  
Teaching Method ◽  
Digital Microscopy ◽  
Human Pathology
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