Characteristics of Lactic Acid Bacteria Isolated from Traditional Wheat Nuruk and their Effects on Nitric Oxide Production in Mouse Macrophage

KSBB Journal ◽  
2018 ◽  
Vol 33 (3) ◽  
pp. 192-199
Author(s):  
Geun-Seop Kim ◽  
Yeong-Ho Choe ◽  
Hong-Kook Kim ◽  
Byeong-Soo Kim
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nitric Oxide Production ◽  
Mouse Macrophage ◽  
Oxide Production

scholarly journals Inhibition of Nitric Oxide Production, Oxidative Stress Prevention, and Probiotic Activity of Lactic Acid Bacteria Isolated from the Human Vagina and Fermented Food

2019 ◽  
Vol 7 (4) ◽  
pp. 109 ◽  
Author(s):  
Chang-Ho Kang ◽  
Seul Hwa Han ◽  
Jin-Seong Kim ◽  
YongGyeong Kim ◽  
Yulah Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging ◽  
Fermented Food ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Probiotic Potential ◽  
Culture Filtrates

In this study, lactic acid bacteria (LAB) with antioxidative and probiotic activities were isolated from the vaginas of Korean women and from fermented food. Among 34 isolated LAB strains, four strains (MG4221, MG4231, MG4261, and MG4270) exhibited inhibitory activity against nitric oxide production. The MG4221 and MG4270 strains were identified as Lactobacillus plantarum, and MG4231 and MG4261 were identified as Lactobacillus fermentum. These strains were able to tolerate pepsin and pancreatin, which is required for probiotic potential. The antioxidant effects of culture filtrates obtained from selected strains included 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity. Most of the culture filtrates had effective DPPH scavenging activity.In conclusion, the selected strains have significant activities and are potentially applicable to the development of functional foods. These strains might also contribute to the prevention and control of several diseases associated with oxidative stress, when used as functional probiotics.

Ex Vivo Effects of Lactobacilli, Streptococci, and Bifidobacteria Ingestion on Cytokine and Nitric Oxide Production in a Murine Model

1999 ◽  
Vol 62 (2) ◽  
pp. 162-169 ◽  
Author(s):  
MARIA VICTORIA TEJADA-SIMON ◽  
ZEYNEP USTUNOL ◽  
JAMES J. PESTKA
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ex Vivo ◽  
Nitric Oxide Production ◽  
Peyer's Patch ◽  
Peritoneal Cells ◽  
Oxide Production ◽  
Tnf Α ◽  
Ifn Γ

Increasing numbers of functional foods and pharmaceutical preparations are being promoted with health claims based on the potential probiotic characteristics of lactic acid bacteria and on their capacity for stimulating the host immune system. However, the specific immune effects of oral administration of these microbes still remains undefined. In this study, we tested the hypothesis that production of immunologic mediators by leukocytes in mice is affected by orally administered lactic acid bacteria. The specific objectives of this study were to evaluate the effects of exposure to eight different lactic acid bacteria in mice on ex vivo cytokine and nitric oxide production in leukocyte cultures. Mice were gavaged with 1 × 109 viable bacteria and peritoneal, Peyer's patch and splenic leukocytes were isolated 8 h later. These were cultured for 2 or 5 days in the presence or absence of mitogens and then interleukin (IL)-6, IL-12, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and nitric oxide production was measured. The results revealed that Lactobacillus acidophilus and L. casei potentiated IL-6 and IL-12 production by peritoneal cells whereas L. acidophilus upregulated IFN-γ and nitric oxide. In contrast, L. helveticus, L. gasseri, L. reuteri, and Bifidobacterium attenuated the production of IL-6, IFN-γ, and nitric oxide by peritoneal cells. TNF-α was not detectable in peritoneal cultures. None of the bacteria altered ex vivo production of cytokines or nitric oxide by Peyer's patch or spleen cell cultures. Taken together, the results suggest that prior oral exposure to lactic acid bacteria could differentially potentiate or attenuate subsequent cytokine and nitric oxide production by peritoneal cells.

scholarly journals Extracelluar Signal-Regulated Kinase-Dependent Nitric Oxide Production from Macrophage-Like Cells by Lactic Acid Bacteria

2009 ◽  
Vol 17 (3) ◽  
pp. 276-281
Author(s):  
Se-Eun Byeon ◽  
Dae-Sung Yoo ◽  
Jae-Hwi Lee ◽  
Suk Kim ◽  
Man-Hee Rhee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Nystatin-induced nitric oxide production in mouse macrophage-like cell line RAW264.7

2009 ◽  
Vol 53 (5) ◽  
pp. 295-300 ◽  
Author(s):  
Naoki Koide ◽  
Yoshikazu Naiki ◽  
Akiko Morikawa ◽  
Gantsetseg Tumurkhuu ◽  
Jargalsaikhan Dagvadorj ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Nitric Oxide Production ◽  
Mouse Macrophage ◽  
Oxide Production

Deoxynivalenol and nivalenol inhibit lipopolysaccharide-induced nitric oxide production by mouse macrophage cells

2010 ◽  
Vol 192 (2) ◽  
pp. 150-154 ◽  
Author(s):  
Kei-ichi Sugiyama ◽  
Masashi Muroi ◽  
Ken-ichi Tanamoto ◽  
Motohiro Nishijima ◽  
Yoshiko Sugita-Konishi
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Mouse Macrophage ◽  
Macrophage Cells ◽  
Oxide Production

scholarly journals Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods

3 Biotech ◽  
2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Chang-Ho Kang ◽  
Jin-Seong Kim ◽  
Hye Min Park ◽  
Seonyoung Kim ◽  
Nam-Soo Paek
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Free Radical ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity

AbstractCompounds of the cell walls of heat-killed lactic acid bacteria show immunomodulatory properties which boost immunological systems, and are used ad postbiotics (paraprobiotics). In this study, we used 17 different heat-killed isolates as postbiotics and evaluated their anti-inflammatory potential on the expression of proinflammatory mediators and cellular signaling pathways of murine macrophage, RAW 264.7 cells. Bifidobacterium bifidum MG731 showed the high 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (90.6%), followed by Bifidobacterium lactis MG741 (59.6%). The Bi. lactis MG741 showed the high ABTS free radical scavenging activity (99.5%), followed by Lactobacillus plantarum MG989 (98.9%), Lactobacillus salivarius MG242 (97.1%), and Bi. bifidum MG731 (96.1%). In addition, Bi. bifidum MG731 showed the lowest nitric oxide production (4.28 µM), followed by B. lactis MG741 (10.80 µM), L. salivarius MG242 (14.60 µM), and L. plantarum MG989 (19.60 µM). The selected strains showed a decreased nitric oxide production via downregulation of inducible nitric oxide synthase and cyclooxygenase 2, which were upregulated via LPS-stimulated RAW 264.7 macrophages. Short-chain fatty acids (SCFA) including acetic, propionic, and butyric acid were produced by four strains. The Bi. bifidum MG731 showed total SCFAs production (4998.6 µg/g), Bi. lactis MG741 (2613.9 µg/g), L. salivarius MG242 (1456.1 µg/g), and L. plantarum MG989 (630.2 µg/g). These results indicated that the various selected strains may possess an anti-inflammatory potential and provide a molecular basis for the development of functional probiotics.

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