scholarly journals Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods

3 Biotech ◽  
2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Chang-Ho Kang ◽  
Jin-Seong Kim ◽  
Hye Min Park ◽  
Seonyoung Kim ◽  
Nam-Soo Paek
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Free Radical ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity

AbstractCompounds of the cell walls of heat-killed lactic acid bacteria show immunomodulatory properties which boost immunological systems, and are used ad postbiotics (paraprobiotics). In this study, we used 17 different heat-killed isolates as postbiotics and evaluated their anti-inflammatory potential on the expression of proinflammatory mediators and cellular signaling pathways of murine macrophage, RAW 264.7 cells. Bifidobacterium bifidum MG731 showed the high 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (90.6%), followed by Bifidobacterium lactis MG741 (59.6%). The Bi. lactis MG741 showed the high ABTS free radical scavenging activity (99.5%), followed by Lactobacillus plantarum MG989 (98.9%), Lactobacillus salivarius MG242 (97.1%), and Bi. bifidum MG731 (96.1%). In addition, Bi. bifidum MG731 showed the lowest nitric oxide production (4.28 µM), followed by B. lactis MG741 (10.80 µM), L. salivarius MG242 (14.60 µM), and L. plantarum MG989 (19.60 µM). The selected strains showed a decreased nitric oxide production via downregulation of inducible nitric oxide synthase and cyclooxygenase 2, which were upregulated via LPS-stimulated RAW 264.7 macrophages. Short-chain fatty acids (SCFA) including acetic, propionic, and butyric acid were produced by four strains. The Bi. bifidum MG731 showed total SCFAs production (4998.6 µg/g), Bi. lactis MG741 (2613.9 µg/g), L. salivarius MG242 (1456.1 µg/g), and L. plantarum MG989 (630.2 µg/g). These results indicated that the various selected strains may possess an anti-inflammatory potential and provide a molecular basis for the development of functional probiotics.

scholarly journals NITRIC OXIDE PRODUCTION AND ANTIOXIDANT ACTIVITY OF DRIED FRUIT EXTRACTS OF TERMINALIA CHEBULA

2018 ◽  
Vol 11 (5) ◽  
pp. 370
Author(s):  
Aridass Dhanasezhian ◽  
Seetharaman Srivani ◽  
Marimuthu Ragavan Rameshkumar
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Scavenging Activity ◽  
Oxide Production

 Objective: The dried ripe fruits of Terminalia chebula have traditionally been used to treat various ailments as it has a wide spectrum of pharmacological activities. Hence, in the present study, we aimed to explore the antioxidant activity, nitric oxide production, cytotoxicity, and phytocompounds present in the aqueous and methanol extract of T. chebula. Methods: The dry fruits of T. chebula were extracted using water and methanol, and the extracts were concentrated by lyophilization method. Phytochemical analysis was done by gas chromatography and mass spectrometry and Fourier-transform infrared spectroscopy. The free radical scavenging activity of T. chebula was estimated by 1,1diphenyl 2, picrylhydrazyl method. RAW 264.7 cells were stimulated with aqueous and methanol extracts, and the production of nitric oxide was estimated by spectrophotometric method using Griess reagent. Cytotoxicity assay was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and percentage of cell viability was calculated.Results: Aqueous and methanolic extracts of the dry fruit of T. chebula showed non-toxic to RAW 264.7 cells at the concentration of 2 mg and 1.5 mg, respectively. These concentrations showed high free radical scavenging activity and production of optimum concentration of nitric oxide in RAW 264.7 cells.Conclusion: Fruit extracts of T. chebula possess properties of nitric oxide production and high free radical scavenging activity; these properties could be useful in the development of immunomodulatory drugs as well as protection against various human diseases associated with oxidative stress.

scholarly journals Free Radical Scavenging Activity of Ethanolic Extract of Citrus paradisi and Naringin -An In vitro Study

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
R Roghini ◽  
K. Vijayalakshmi
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Citrus Paradisi

The present study attempts to find naturally-occurring antioxidants of fruit-based which give efficacy by additive activities. Citrus paradisi, known as Grape fruit contains significant bioactive components such as Naringin. The present study examines the free radical scavenging activity of ethanolic extract of Citrus paradisi and Naringin. The study was carried out with different radical scavenging assays like hydroxyl, DPPH, hydrogen peroxide, nitric oxide, super oxide. Citrus paradisi extracts showed lower radical scavenging activities in assays such as DPPH, superoxide and hydroxyl when compared with Naringin. Naringin showed the higher radical scavenging effect with nitric oxide, and hydrogen peroxide in comparison with citrus paradisi extract. However, both were analysed by using ascorbic acid as standard. The current study gives evidence that both showed potential free radical scavenging activity.

scholarly journals EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF MIMOSA PUDICA THORNS

2018 ◽  
Vol 11 (11) ◽  
pp. 153 ◽  
Author(s):  
Lakshmibai R ◽  
Amirtham D
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Mimosa Pudica ◽  
Age Related ◽  
Dpph Method

Objective: Mimosa pudica is a traditional medicinal plant. The natural antioxidant compounds from plants mop up the free radicals causing cell damage and maintain the biological systems. The aim of the present study is to evaluate the free radical scavenging potential of the ethanolic and aqueous thorn extracts of M. pudica.Methods: The ethanolic and aqueous thorn extracts of M. pudica were analyzed for the phytochemicals and for free radical scavenging activity by 1, 1 diphenyl 1-2-picric hydrazine (DPPH), 2, 2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and nitric oxide free radical scavenging method.Results: Flavonoids, saponins, glycosides, alkaloids, terpenoids, and coumarins were the phytoconstituents revealed in ethanolic and aqueous thorn extracts of M. pudica. At 250 μg/mL concentrations, aqueous thorn extracts of M. pudica exhibited 73.41% radical scavenging activity by DPPH method and 26.10% inhibition by nitric oxide free radical scavenging method. However, the ethanolic extracts of M. pudica thorns exhibited 73.35% inhibition by ABTS free radical scavenging method at 250 μg/mL concentrations.Conclusion: The results obtained suggest that the plant extracts from M. pudica could serve as a potential source of antioxidant in slowing down the process of aging and age-related or oxidative stress-related degenerative diseases. Moreover, the isolation of bioactive principle responsible for the antioxidant activity and formulation of novel therapeutic agents can be further studied.

scholarly journals IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES

2020 ◽  
pp. 92-95
Author(s):  
ANITHA P ◽  
NAZEEMA TH
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Glutathione Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Enzymic Antioxidants

Objectives: This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of Crescentia cujete. Methods: Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of C. cujete leaf. Results: The leaf extract of C. cujete showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of C. cujete leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid. Conclusion: From the results, it has concluded that the ethanol extract of the C. cujete leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.

scholarly journals Evaluation of Free Radical Scavenging Potential of Different Bioactive Fractions Present in Boerhavia diffusa Linn. Root Extract: An in-vitro Approach

2020 ◽  
pp. 99-107
Author(s):  
Mohammad Khalid ◽  
Mohammed H. Alqarni ◽  
Ahmed I. Foudah ◽  
Juber Akhtar ◽  
Badruddeen . ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Chloroform Fraction ◽  
Scavenging Activity ◽  
Boerhavia Diffusa

Aim and Objectives: Boerhavia diffusa (B. diffusa) frequently known as punarnava is specifically used to replenish the body. The present work was designed to evaluate the scavenging potential of its bioactive constituents. Materials and Methods: The different fractions of B. diffusa root methanolic extract were examined for phenolic, flavonoids contents, DPPH free radical and Nitric oxide scavenging activities. Further antioxidant activity was evaluated by ABTS free radical scavenging method and also from the reducing potential scavenging activity. The total phenolic content in different fractions by using various solvent like ethanol, diethyl ether, chloroform, ethyl acetate, and n-butanol were carried out to find the antioxidant activities. Results: The phenolic content was highest in ethanolic fraction that was significantly comparable with ascorbic acid. The flavonoid content was highest in ethanol fraction (41.93 ± 3.92 µg/mL) followed by n-butanol fraction (31.68 ± 1.72 µg/mL), then ethyl acetate fraction (29.67 ± 2.83 µg/mL) and least in chloroform fraction (16.91 ± 2.74 µg/mL). The ethanolic fraction of B. diffusa also showed highest DPPH radical scavenging activity (101.29 ± 3.78) when compared with other fractions of same extract using different solvent phases. Moreover the nitric oxide scavenging activity of ethanolic fraction was maximum (82.31 ± 2.83) than different fractions. The ethanolic fraction also showed improved ABTS radical scavenging activity (81.73 ± 2.73 mg/mL) while chloroform fraction showed poor ABTS radical cation scavenging activity (29.51 ± 2.79 mg/mL). Conclusions: The study concludes that Boerhavia diffusa has rich and ample source of phenolic acid and flavonoids. Among all fractions the ethanolic has potent antioxidant activity, which shows its significance for a better novel approach.

scholarly journals Antioxidant Screening on the Whole Plant of Cyanthillium cinereum (L) H.Rob

2021 ◽  
Vol 8 (7) ◽  
pp. 458-461
Author(s):  
Mohammed Shihab K. K. ◽  
Rajagopal P. L. ◽  
Nasila K.
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Whole Plant

Methanolic extract of the whole plant of Cyanthillium cinereum (L) H.Robwasevaluated for free radical scavenging activity by using Nitric oxide scavenging activity (NO) and Reduction of 1, 1-Diphenyl -2-Picryl Hydrazyl (DPPH) free radical scavenging activity. Throughout the studies the plant extract showed potent antioxidant activity and the activity was found to be concentration dependent which may be attributed to the high flavonoid content of the plant. Keywords: Cyanthillium cinereum (L) H.Rob, DPPH, NO.

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF SUCCESSIVE EXTRACTS OF ALYSICARPUS VAGINALIS VAR. NUMMULARIFOLIUS (DC.) MIQ

2017 ◽  
Vol 9 (6) ◽  
pp. 231
Author(s):  
Susan Kurian ◽  
Leenamma Joseph ◽  
Josekumar V. S.
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Objective: The present study was carried to evaluate free radical scavenging activity and phytochemical analysis of successive extraction with hexane (AVH), ethyl acetate (AVE) and methanol (AVM) of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ., a herb used in home remedies.Methods: The entire plant was dried and powdered, successively extracted (soxhlet apparatus) and concentrated using rotary vacuum evaporator. The extracts were quantitatively analyzed for phytochemicals like total phenolic, flavonoids, alkaloids and carbohydrates following standard methods. The free radical scavenging activity was evaluated with DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide radical and hydrogen peroxide scavenging reactions.Results: The quantitative phytochemical studies of the crude extracts showed that AVM extract with highest phenolic, flavonoid, alkaloid and carbohydrate content which is followed by AVE and AVH. The IC50 values of AVH, AVE and AVM for scavenging DPPH, nitric oxide and hydrogen peroxide were 589.21±0.09 µg/ml, 254.65±0.08 µg/ml and 261.40±0.08 µg/ml; 533.81±0.09 µg/ml, 362.07±0.09 µg/ml and 456.36±0.08 µ/ml; 464.66±0.08 µg/ml, 380.29±0.08 µg/ml and 367.6±0.08 µg/ml respectively. Conclusion: The present study revealed that AVM extracts having high polyphenolic compounds when compared with AVE and AVH. The free radical scavenging reaction was greater in ethyl acetate and methanol extracts. This suggests that due to the high antioxidant reactions, these extracts can be useful for the treatment of oxidative stress–related diseases.

scholarly journals Free Radical Scavenging Activity of Aerial Parts of Artemisia pallens in Mediating Hepatoprotective Activity of RIF+INH Intoxicated Rats

2019 ◽  
Vol 14 ◽  
pp. 1-15
Author(s):  
Praveen Kumar Ashok ◽  
Poonam Rishishwar ◽  
Kumud Upadhyaya
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dry Extract ◽  
Artemisia Pallens

To investigate the free radical scavenging & hepatoprotective activity of phenolic rich fraction of Artemisia pallens on RIF+INH induced oxidative stress in Sprague dawley rats. Free radical scavenging and hepatoprotective activity was evaluated by using DPPH, Nitric oxide, Superoxide radical and Hydroxyl radical assay models and induced RIF+INH intoxicated rats. The total phenolic content was found to be 312.60 μg gallic acid equivalents (GAE)/gm of dry extract. The total flavonoid content was found to be 322.20 μg rutin equivalents (RUE)/gm of dry extract. In the current study, free radical scavenging activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, superoxide radical and hydroxyl radical scavenging activity were found to be (42.25 ± 0.95) and (09.16 ± 1.62) μg/ml, (101.62 ± 1.64) and (32.41 ± 1.24) μg/ml, (72.62 ± 1.86) and (10.28 ± 1.96) μg/ml, (33.82 ± 1.12) and (12.82 ± 1.86) μg/ml, respectively. There was also a dose dependent increase in reductive ability of Artemisia pallens extract with increase in concentration and were further investigated in invivo hepatoprotective activity experiment against toxicity induced by RIF+INH. The free radical scavenging and hepatoprotective activity may be attributed to the presence of phenolic compounds and histology of the liver section of the animals treated with the extracts showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity of Artemisia pallens.

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