Determination of Epidemiology of Clinically Isolated Cryptococcus neoformans Strains in Japan by Multilocus Sequence Typing

A worrisome phenomenon is the progressive global spread of Enterobacteriaceae in poultry and chicken meat expressing plasmid-mediated enzymes that inactivate β-lactam antibiotics, suggesting that the food chain might play a role in the epidemiology and the transmission of extended-spectrum cephalosporin-resistant Enterobacteriaceae to humans. The aim of the present study was to further characterize 24 extended-spectrum cephalosporin-resistant Enterobacteriaceae isolated from domestic and imported poultry meat by antibiotic susceptibility testing, identification of the blaESBL/blapAmpC genes, conjugation mating experiments and determination of plasmid incompatibility types, multilocus sequence typing, and analysis of the Escherichia coli phylogenetic groups. On account of their resistance patterns, 21 of the total 24 isolates were classified as multidrug resistant. Eleven isolates carried a blaCMY-2 gene, whereas 13 isolates harbored a blaCTX-M-1 gene. All isolates harbored plasmids that were assigned to 8 of the 18 described plasmid incompatibility groups, the most frequent of which were IncI1, IncFIB, IncB/O, and IncFrepB. The blaESBL/blapAmpC genes were harbored mainly by transferable IncI1 and IncB/O plasmids. Multilocus sequence typing as well as E. coli phylogenetic group typing revealed a high heterogenicity even among different isolates of the same sample.

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A DNA electrochemical biosensor based on homogeneous hybridization for the determination of Cryptococcus neoformans

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2018.09.001 ◽

2018 ◽

Vol 827 ◽

pp. 27-33 ◽

Cited By ~ 6

Author(s):

Yin-Huan Liu ◽

Hao-Hua Deng ◽

Hong-Na Li ◽

Teng-Fei Shi ◽

Hua-Ping Peng ◽

...

Keyword(s):

Cryptococcus Neoformans ◽

Electrochemical Biosensor ◽

Dna Electrochemical Biosensor

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Characterization of Cryptococcus neoformans isolated from urban environmental sources in Goiânia, Goiás State, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652005000400005 ◽

2005 ◽

Vol 47 (4) ◽

pp. 203-207 ◽

Cited By ~ 24

Author(s):

Cláudia Castelo Branco Artiaga Kobayashi ◽

Lúcia Kioko Hasimoto e Souza ◽

Orionalda de Fátima Lisboa Fernandes ◽

Sula Cristina Assis de Brito ◽

Ana Cláudia Silva ◽

...

Keyword(s):

Cryptococcus Neoformans ◽

Cryptococcus Species ◽

Major Route ◽

Opportunistic Fungal Pathogen ◽

Ornamental Trees ◽

Pigeon Dropping ◽

Vegetable Material

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis as the most frequent clinical presentation in immunocompromised patients, mainly in people infected by HIV. This fungus is an environmental encapsulated yeast, commonly found in soil enriched with avian droppings and plant material. A total of 290 samples of pigeon and the other avian droppings, soil, ornamental trees and vegetable material associated with Eucalyptus trees were collected to study environmental sources of Cryptococcus species in Goiânia, Goiás State. The determination of varieties, serotypes and the susceptibility in vitro to fluconazole, itraconazole and amphotericin B of C. neoformans isolates were performed. C. neoformans var. grubii (serotype A) was found in 20.3% (36/177) of pigeon dropping samples and in 14.3% (5/35) of samples of Eucalyptus. None of the environmental isolates of C. neoformans showed in vitro resistance to three antifungal agents. The knowledge of major route for human cryptococcal infection (inhalation of infectious particles from saprophytic sources) and a total of 60 C. neoformans isolates obtained from AIDS patients with cryptococcal meningitis between October 2001 and April 2002 justify the study of the habitats of these yeasts as probable sources of cryptococcosis in this city.

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Genotypic diversity in clinical and environmental isolates of Cryptococcus neoformans from India using multilocus microsatellite and multilocus sequence typing

Mycoses ◽

10.1111/myc.13041 ◽

2020 ◽

Vol 63 (3) ◽

pp. 284-293 ◽

Cited By ~ 1

Author(s):

Anupam Prakash ◽

Gandhi Sundar ◽

Brijesh Sharma ◽

Ferry Hagen ◽

Jacques F. Meis ◽

...

Keyword(s):

Cryptococcus Neoformans ◽

Multilocus Sequence Typing ◽

Genotypic Diversity ◽

Environmental Isolates

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New multilocus sequence typing primers to enable genotyping of AD hybrids within the Cryptococcus neoformans species complex

Medical Mycology ◽

10.1093/mmy/myaa047 ◽

2020 ◽

Vol 58 (7) ◽

pp. 1005-1009

Author(s):

Massimo Cogliati ◽

Frederic Roger ◽

Wieland Meyer ◽

Vincent Robert ◽

Sébastien Bertout

Keyword(s):

Molecular Epidemiology ◽

Cryptococcus Neoformans ◽

Multilocus Sequence Typing ◽

Species Complex ◽

Mlst Database ◽

Specific Primers ◽

Typing Method ◽

Genetic Studies ◽

Epidemiology Studies ◽

Ad Hybrids

Abstract Although AD hybrids within the Cryptococcus neoformans species complex represent about 20% of the isolates identified in Europe, phylogenetic and population genetic studies are lacking due to the inability to use the standardized typing method. The aim of the present study was to design new molecular type specific primers in order to apply the standard ISHAM consensus multilocus sequence typing (MLST) scheme to AD hybrids. The new primers are able to specifically amplify VNI and VNIV alleles of the seven MLST loci in both haploid and diploid or aneuploid hybrid strains. This study forms the basis for future molecular epidemiology studies of AD hybrids. Lay abstract We designed and tested new specific primers to amplify the two alleles of each of the seven MLST loci in C. neoformans species complex hybrids. The sequences obtained from hybrids can be compared with those present in the Cryptococcus global MLST database for future molecular epidemiology studies.

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