scholarly journals MORPHOLOGICAL ANOMALIES IN ADULT OYSTER, SCALLOP, AND ATLANTIC SILVERSIDES EXPOSED TO WASTE MOTOR OIL

1975 ◽  
Vol 1975 (1) ◽  
pp. 473-477 ◽  
Author(s):  
George R. Gardner ◽  
Paul P. Yevich ◽  
Peter F. Rogerson

ABSTRACT Waste motor oil concentrations of 20 ppm and higher induced lesions in the vascular systems of Atlantic silversides and oyster. These lesions were associated with the pseudobranch, the heart, and the arterial system of the Atlantic silversides, and the branchial efferent vein of the oyster. Lesions occurred in the gastro-intestinal tract of oyster, the gill and kidney of scallop, and the mantle of both species. Based on these preliminary exposures, the scallop, of the three species tested, demonstrated the highest sensitivity to the toxic effects of 20 ppm waste motor oil, or higher, for periods up to 60 days. The LC-50 (96 hr) value for waste motor oil and adult Atlantic silversides was 1,7000 ppm. Acute toxicity values were not determined for the oyster or scallop.

2014 ◽  
Vol 9 (3) ◽  
pp. 196-204 ◽  
Author(s):  
Lucia Montenegro ◽  
Giuseppe Losurdo ◽  
Raffaele Licinio ◽  
Maria Zamparella ◽  
Floriana Giorgio ◽  
...  

2021 ◽  
Vol 9 (5) ◽  
pp. 1013
Author(s):  
Karina Arellano-Ayala ◽  
Juhwan Lim ◽  
Subin Yeo ◽  
Jorge Enrique Vazquez Bucheli ◽  
Svetoslav Dimitrov Todorov ◽  
...  

Preservation of probiotics by lyophilization is considered a method of choice for developing stable products. However, both direct consumption and reconstitution of dehydrated probiotic preparations before application “compromise” the survival and functional characteristics of the microorganisms under the stress of the upper gastro-intestinal tract. We evaluated the impact of different food additives on the viability, mucin adhesion, and zeta potential of a freeze-dried putative probiotic, Lactiplantibacillus (Lp.) plantarum HAC03. HAC03-compatible ingredients for the formulation of ten rehydration mixtures could be selected. Elevated efficacy was achieved by the B-active formulation, a mixture of non-protein nitrogen compounds, sugars, and salts. The survival of Lp. plantarum HAC03 increased by 36.36% compared rehydration with distilled water (4.92%) after passing simulated gastro-intestinal stress conditions. Cell viability determined by plate counting was confirmed by flow cytometry. B-active formulation also influenced Lp. plantarum HAC03 functionality by increasing its adherence to a Caco-2 cell-line and by changing the bacterial surface charge, measured as zeta potential.Hydrophobicity, mucin adhesion and immunomodulatory properties of Lp. plantarum HAC03 were not affected by the B-active formulation. The rehydration medium also effectively protected Lp. plantarum ATCC14917, Lp. plantarum 299v, Latilactobacillus sakei (Lt.) HAC11, Lacticaseibacillus (Lc.) paracasei 532, Enterococcus faecium 200, and Lc. rhamnosus BFE5263.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1824
Author(s):  
Giorgia Corazzola ◽  
Matteo Baini ◽  
Carla Grattarola ◽  
Cristina Panti ◽  
Federica Marcer ◽  
...  

Organs and content of the gastro-intestinal tract (GIT) of marine mammals are relevant for a variety of investigations and provide data to researchers from different fields. Currently used protocols applied to the GIT for specific analysis limit the possibility to execute other investigations and important information could be lost. To ensure a proper sample collection and a multidisciplinary investigation of the GIT of marine mammals, a new multi-sieves tool and a specific protocol have been developed. This new device and approach allowed the simultaneous sampling of the GIT and its content for the main investigations concerned. The samples collected during these preliminary trials were suitable to perform all the different research procedures considered in this work. The obtained results show that with a few and easy procedural adjustments, a multidisciplinary sampling and evaluation of the GIT of marine mammals is possible. This will reduce the risk of losing important data aimed at understanding the cause of death of the animal, but also biology and ecology of marine mammals, and other important data for their conservation and habitats management.


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