scholarly journals Assessment of the Exposure Risk to Mycotoxins from Stored Maize (Zea mays L.) in Triple Bags with Aromatic Plants Leaves (Lippia multiflora and Hyptis suaveolens) in Côte d’Ivoire

Author(s):  
Yao Vanessa Gaël ◽  
G. Henri Marius Biego ◽  
Konan K. Constant ◽  
Coulibaly Adama ◽  
Sidibe Daouda

In Côte d’Ivoire, maize is second cereal most cultivated and consumed after rice. In bad storage conditions, corn may be contaminated by mycotoxins (aflatoxin B1, total aflatoxins, ochratoxin A, fuminosin B1, zearalenone). The aim of this study was to assess the exposure risk of these mycotoxins for maize stored in triple bags in presence of aromatic plants leaves (Lippia multiflora and Hyptis suaveolens). The contents of water activity, aflatoxin B1 (AFB1), total aflatoxins (AFT), ochratoxin A (OTA), fuminosin B1 (FB1) and zearalenone (ZEA) were studied and monitored. The levels of AFB1, AFT, OTA, FB1 and ZEA resulted from maize grains treated with plants leaves were significantly lower than those recorded with untreated maize of control bags. The estimated daily intakes in AFB1, AFT, OTA, FB1 and ZEA, deriving with consumption of maize from experimental batches stored for 18 months are respectively 1.69 ± 0.00 - 2.09 ± 0.01 ng/kg bw/day, 8.66 – 10.91 ng/kg bw/day, 1.86 ± 0.01 - 2.47 ± 0.01 ng/kg bw/day, 2.01 ± 0.05 - 3.01± 0.05 ng/kg bw/day and 1.89 ± 0.12 - 3.56 ± 0.04 ng/kg bw/day. These levels are lower than the estimated intakes from maximal reference value (EDILM) for OTA, FB1 and ZEA. For aflatoxin B1 and total aflatoxins, the estimated daily intakes are lower than the estimated intakes from maximal reference value (EDILM) during, respectively, 15 storage months and 10 storage months. However, after 18 storage months, exposure risk of aflatoxin B1 is higher than the estimated intakes from maximal reference value. This inexpensive and easy-to-use treatment should be popularized among farmers

2006 ◽  
Vol 23 (10) ◽  
pp. 1000-1007 ◽  
Author(s):  
Béatrice Sangare-Tigori ◽  
Serge Moukha ◽  
H. James Kouadio ◽  
Anne-Marie Betbeder ◽  
Djédjé Sébastien Dano ◽  
...  

Author(s):  
Ehouman Ano Guy Serge ◽  
Yao N’Zue Benjamin ◽  
Hampoh Ade Hortense ◽  
Fofana Alimata ◽  
Traore Karim Sory ◽  
...  

Author(s):  
Ehouman Ano Guy Serge ◽  
Kouassi Kouassi Clément ◽  
Kouassi Kra Athanase ◽  
Ehui Edi Jean Fréjus ◽  
Beugre Grah Avit Maxwell ◽  
...  

The mycotoxins are toxic composites frequently found in foodstuffs causing some damages to consumers. This current study has been realised in order to estimate the content of Aflatoxin B1 and Ochratoxin A in dry okra and cassava chips frequently consumed by the population of Daloa. The analysis by chromatography in the liquid phase at high performance has shown that these foodstuffs contain Aflatoxin B1 and Ochratoxin A. These results reveal an average content of : 2.58 ± 3.65 µg/Kg and 3.08 ± 5.04  µg/Kg for the Aflatoxin B1 ; 1.06 ± 0.86 µg/Kg and 0.61±0.24 µg/Kg for the Ochratoxin A respectively in the flour of cassava chips and the powder of dry okra. These concentrations are somewhat to higher than those allowed by the norm of Codex Alimentarius. Under this form, the consumption of these foodstuffs by the population expose them at risk of diseases.


Author(s):  
Yao Vanessa Gaël ◽  
Konan K. Constant ◽  
Niamketchi G. Leonce ◽  
A. Aka Boigny Ange ◽  
Adama Coulibaly ◽  
...  

The aim of study was to evaluate merchantability quality of stored maize in triple bagging with biopesticides. Maize grains were collected in March 2016 in the north of Côte d’Ivoire. The fresh leaves of Lippia multiflora and Hyptis suaveolens were collected and dried in sunlight for 7 days in the center of Cote d'Ivoire. Triple bags were bought in Abidjan market. All this material was sent to the Laboratory of Biochemistry and Food Sciences, Félix Houphouët-Boigny University, Côte d’Ivoire, to perform the experiment. Ten treatments were obtained for the experimentation. The first treatment was conservation of 50 kg of maize grain in a polypropylene bag. The second treatment was conservation of 50 kg of maize grain in a PICS bag. The other eight treatments were carried out with PICS bags each containing 50 kg of maize grain and different proportions of chopped leaves Lippia multiflora and Hyptis suaveolens. A central composite design was used for sample constitution. Thus, a control group with polypropylene bags (TPPB0), a control group in PICS bags without biopesticides (TPB0) and 8 experimental lots of triple bags noted TB1 containing 0.625 kg L. multiflora and 0.625 kg H. suaveolens, TB2 with 0.40 kg of L. multiflora and 1.60 kg of H. suaveolens, TB3 with 1.60 kg of L. multiflora and 0.40 kg of H. suaveolens, TB4 with 0.10 kg of L. multiflora and 0.40 kg of H. suaveolens, TB5 with 0.40 kg of L. multiflora and 0.10 kg of H. suaveolens, TB6 with 2.5 kg of L. multiflora and 2.5 kg of H. suaveolens, TB7 with 1.25 kg of L. multiflora and TB8 with 1.25 kg of H. suaveolens have been used. Changes in moisture, damages and weight losses were studied. The results show moisture levels (from 09.02±0.11% to 12.07± 0.06%), weight loss (from 0.49±0.02% to 2.54±0.07%) and damage (from 0.99±0.02% to 3.96± 0.01%), corn stored in triple bagged bags with different proportions of biopesticide were significantly lower than those recorded in the Polypropylene woven sample bag (TPPB0) and in the triple bagged control bag during the storage period. The results obtained indicate stability in the quality of maize stored for 18 months in triple bagged bags containing different proportions of leaves of L. multiflora and H. suaveolens. A proportion of 5% of the mixture of leaves of L. multiflora and H. suaveolens (2.5 kg of L. multiflora and 2.5 kg of H. suaveolens) in triple bagged bags is recommended for a better preservation of the merchantability of the stored maize grains kernels. Storage of maize grains in PICS bags with the leaves of L. multiflora and H. suaveolens appears as a method of effective and inexpensive conservation to ensure the merchantability quality of maize.


2020 ◽  
pp. 22-33
Author(s):  
Yao Vanessa Gaël ◽  
G. Henri Marius Biego ◽  
Konan K. Constant ◽  
Niamketchi G. Leonce ◽  
Adama Coulibaly

In Côte d’Ivoire, maize plays an essential role as subsistence, commercial and socio-cultural culture. To consume good quality corn, it is necessary to preserve the sanitary quality through a good storage method. The aim of study was to evaluate sanitary quality of stored maize in triple bags containing plants biopesticides. Maize grains were collected in March 2016 in the north of Côte d’Ivoire. The fresh leaves of Lippia multiflora and Hyptis suaveolens were collected and dried in sunlight for 7 days in the center of Côte d'Ivoire. Triple bags were bought in Abidjan market. All this material was sent to the Laboratory of Biochemistry and Food Sciences, Félix Houphouët-Boigny University, Côte d’Ivoire, to perform the experiment. A central composite design was used for sample constitution. Ten treatments were obtained for the experimentation. The first treatment was conservation of 50 kg of maize grain in a polypropylene bag. The second treatment was conservation of 50 kg of maize grain in a triple bag. The other eight treatments were carried out with PICS bags each containing 50 kg of maize grain and different proportions of chopped leaves Lippia multiflora and Hyptis suaveolens. Thus, a control group with polypropylene bag (TPPB0), a control group in triple bag without biopesticides (TPB0) and 8 experimental lots of triple bags noted TB1 containing 0.625kg L. multiflora and 0.625kg H. suaveolens, TB2 with 0.40 kg of L. multiflora and 1.60 kg of H. suaveolens, TB3 with 1.60 kg of L. multiflora and 0.40 kg of H. suaveolens, TB4 with 0.10 kg of L. multiflora and 0.40 kg of H. suaveolens, TB5 with 0.40 kg of L. multiflora and 0.10 kg of H. suaveolens, TB6 with 2.5 kg of L. multiflora and 2.5 kg of H. suaveolens, TB7 with 1.25 kg of L. multiflora and TB8 with 1.25 kg of H. suaveolens have been used. The contents of moisture, water activity, aflatoxin B1 (AFB1), ochratoxin A (OTA), fuminosin B1 (FB1) and zearalenone (ZEA) were studied. The levels of AFB1, OTA, FB1 and ZEA resulted from maize grains treated with biopesticides were significantly lower than those recorded with untreated maize of control bags. The results show AFB1 levels (from 4.17 ± 0.05 to 5.15 ± 0.06 μg/kg), OTA levels (from to 4.58 ± 0.25 to 6.10 ± 0.01 μg/kg), FB1 levels (from 4.96 ± 0.07 μg/kg to 7.42 ± 0.06 μg/kg) and ZEA levels (from 4.66 ± 0.10 μg/kg to 8.78 ± 0.14 μg/kg). Maize samples stored in triple bagged bags with different proportions of biopesticide were significantly lower than those recorded in the polypropylene woven sample bag (TPPB0) and in the triple bagged control bag (TPB0) during the storage period. Storage of maize grains in triple bags with the leaves of L. multiflora and H. suaveolens appears as a method of effective and inexpensive conservation to ensure the sanitary quality of maize. This inexpensive and easy-to-use treatment should be popularized among farmers.


2021 ◽  
Vol 19 (1) ◽  
pp. 653-664
Author(s):  
Grażyna Kowalska ◽  
Radosław Kowalski

Abstract The objective of this study was the estimation of the content of 13 mycotoxins (diacetoxyscirpenol, T-2 toxin, HT-2 toxin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenone X, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, and zearalenone) in various products from the eastern part of Poland. The content of mycotoxins in the analysed samples was assayed using the extraction method combined with HPLC-MS/MS analysis. We found mycotoxins in 25 of the 92 samples tested (27%). Contamination with mycotoxins was noted most frequently in samples of cereals – 56% – and also in samples of flour and cocoa, in which a content of mycotoxins was noted in 24 and 16% of the samples, respectively. The most frequently identified were the following – deoxynivalenol detected in 18 samples (72%), zearalenone detected in eight samples (32%), toxin HT-2 detected in four samples (16%), ochratoxin A identified in three samples (12%), and toxin T-2 detected in one sample (4%). In one analysed sample of mixed flour and in one analysed sample of wheat and rye flour, the maximum allowable concentration was exceeded in the case of two identified mycotoxins – deoxynivalenol (2,250 μg/kg) and ochratoxin A (15.6 and 17.1 μg/kg).


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