scholarly journals Multiplex PCR Assay for Detection of Pneumococcal Serotypes in Nasopharyngeal Samples of Healthy Children; Tehran, 2009-2010

2014 ◽  
Vol 4 (24) ◽  
pp. 3780-3790 ◽  
Author(s):  
S. Tabatabaei
2016 ◽  
Vol 54 (8) ◽  
pp. 2197-2200 ◽  
Author(s):  
José M. Marimón ◽  
María Ercibengoa ◽  
Erica Santacatterina ◽  
Marta Alonso ◽  
Emilio Pérez-Trallero

For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.


2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42


Author(s):  
Sunarno ◽  
Khariri ◽  
Fauzul Muna ◽  
Kambang Sariadji ◽  
Yuni Rukminiati ◽  
...  

2010 ◽  
Vol 105 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Mollah Md. Hamiduzzaman ◽  
Ernesto Guzman-Novoa ◽  
Paul H. Goodwin

2012 ◽  
Vol 65 (4) ◽  
pp. 361-368 ◽  
Author(s):  
R. Tolentino-Ruiz ◽  
D. Montoya-Varela ◽  
M. García-Espitia ◽  
M. Salas-Benito ◽  
A. Gutiérrez-Escolano ◽  
...  

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