scholarly journals Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes

2016 ◽  
Vol 54 (8) ◽  
pp. 2197-2200 ◽  
Author(s):  
José M. Marimón ◽  
María Ercibengoa ◽  
Erica Santacatterina ◽  
Marta Alonso ◽  
Emilio Pérez-Trallero
Keyword(s):  
Capillary Electrophoresis ◽  
Multiplex Pcr ◽  
Disease Surveillance ◽  
Pneumococcal Disease ◽  
Cost Effective ◽  
Single Step ◽  
Pneumococcal Serotypes ◽  
Pcr Assay ◽  
Single Tube ◽  
Multiplex Pcr Assay

For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.

scholarly journals Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

2000 ◽  
Vol 38 (11) ◽  
pp. 4114-4120 ◽  
Author(s):  
WanHong Xu ◽  
Mike C. McDonough ◽  
Dean D. Erdman
Keyword(s):  
Multiplex Pcr ◽  
Human Adenovirus ◽  
Cost Effective ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Amplicon Size ◽  
Species Specific ◽  
Fiber Gene ◽  
Amplification Reaction

A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.

scholarly journals SO002QUANTIFICATION OF PLASMA DONOR-DERIVED CELL-FREE DNA TO MONITOR KIDNEY TRANSPLANT HEALTH: PRELIMINARY RESULTS OF A SINGLE TUBE MULTIPLEX PCR ASSAY

2016 ◽  
Vol 31 (suppl_1) ◽  
pp. i1-i1 ◽  
Author(s):  
Els Gielis ◽  
Kristien Ledeganck ◽  
Hans Wils ◽  
Jean-Louis Bosmans ◽  
Steven Van Laecke ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Kidney Transplant ◽  
Pcr Assay ◽  
Cell Free Dna ◽  
Single Tube ◽  
Preliminary Results ◽  
Free Dna ◽  
Multiplex Pcr Assay ◽  
Plasma Donor

Single tube multiplex PCR assay for the identification of banned meat species

2020 ◽  
Vol 13 (4) ◽  
pp. 284-291
Author(s):  
Memoona Iqbal ◽  
Muhammad Sulyman Saleem ◽  
Muhammad Imran ◽  
Waseem Ahmad Khan ◽  
Kamran Ashraf ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Multiplex Pcr Assay ◽  
Meat Species

A multiplex PCR assay for the simultaneous detection and discrimination of the sevenEimeriaspecies that infect domestic fowl

Parasitology ◽  
2003 ◽  
Vol 127 (4) ◽  
pp. 317-325 ◽  
Author(s):  
S. FERNANDEZ ◽  
A. H. PAGOTTO ◽  
M. M. FURTADO ◽  
Â. M. KATSUYAMA ◽  
A. M. B. N. MADEIRA ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Domestic Fowl ◽  
Detection Threshold ◽  
Simultaneous Detection ◽  
Cost Effective ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Sensitivity Tests ◽  
Different Sources ◽  
Single Tube Reaction

This study reports the development of a novel multiplex PCR assay based on SCAR (Sequence-Characterised Amplified Region) markers for the simultaneous diagnosis of the 7Eimeriaspecies that infect domestic fowl. Primer pairs specific for each species were designed in order to generate a ladder of amplification products ranging from 200 to 811 bp. Sensitivity tests for each species were carried out, showing a detection threshold of 1–5 pg, which corresponds approximately to 2–8 sporulated oocysts. Distinct isolates of the 7Eimeriaspecies from different geographical sources were tested and successfully detected by the assay. All the species were amplified homogeneously, whether or not one of them was present in a high quantity, indicating that there was no cross-interference. The assay was also tested with different sources ofTaqDNA polymerase and thermocycler models, confirming the high reproducibility of the reaction. The economy of consumables and labour represented by a single-tube reaction greatly facilitates the molecular diagnosis of a large number of samples, making it appropriate for field epizootiological surveys. We propose the use of this multiplex PCR assay as a rapid and cost-effective diagnostic method for the detection and discrimination of the 7Eimeriaspecies that infect domestic fowl.

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