scholarly journals Optimisation of Lactic Acid Fermentation from Cassava Peel by Lactobacillus casei (ATCC334)

2021 ◽  
pp. 29-42
Author(s):  
Rahmat Folashade Zakariyah ◽  
Micheal Oluwaseyi Ojo ◽  
Kamoldeen Abiodun Ajijolakewu ◽  
Kudirat Bolanle Saliu ◽  
Risikat Nike Ahmed ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Nitrogen Source ◽  
Substrate Concentration ◽  
Lactobacillus Casei ◽  
Inoculum Size ◽  
Lactic Acid Fermentation ◽  
Partial Purification ◽  
Acid Fermentation ◽  
Cassava Peel ◽  
Source Concentration

The demand for lactic acid is steadily increasing due to the desire of its bioproduction over chemical synthesis. The associated cost, however, is a significant hurdle. This study reports lactic acid fermentation by Lactobacillus casei ATCC334 from cassava peel. It investigates the effect of unhydrolysed cassava peels, acidic, alkali hydrolysates; fermenting pH; substrate concentration; nitrogen source concentration; duration; and inoculum size. An attempt at a cheaper purification and recovery protocol relative to those currently in use was similarly performed. Acidic hydrolysate yielded 10.53%, unhydrolysed substrate gave 4.80% with alkali hydrolysate yielding 4.75%. The highest LA yield was obtained at pH 6.0, 2.0% v/v inoculum size, 25% w/v substrate concentration, 5% nitrogen source concentration. A post-optimisation combination yielded 18.3% LA suggesting that one-factor-at-a-time may be unsuitable for optimisation studies involving cassava peel and L. casei ATCC334. FTIR spectra of product suggests effective partial purification. Hence, an improvement in the optimization strategy for production is recommended for subsequent study.

scholarly journals A study on Lactic Acid fermentation of Sugar Palm (Arenga Pinnata) sap by Lactobacillus Casei

2014 ◽  
Vol 17 (2) ◽  
pp. 65-71
Author(s):  
Nhat Minh Dang ◽  
Trung Quang Nguyen
Keyword(s):  
Lactic Acid ◽  
Calcium Carbonate ◽  
Ammonium Sulphate ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Microbial Culture ◽  
Acid Fermentation ◽  
Lower Ability

This study aimed to evaluate the effect of various factors on the yield of lactic acid fermentation using sap from sugar palm and Lactobacillus casei. The sugar palm sap after harvesting, pretreatment was added with ammonium sulphate, calcium carbonate and microbial culture at density of 109 cells/ml and let fermented for acid lactic production. The results of the experiments showed that the culture size, amount of added ammonium sulphate and calcium carbonate had significant effect on lactic acid production. The most appropriate parameters determined were culture size of 5%, ammonium sulphate of 3.0 g/l and calcium carbonate of 4.0 g/l. Meanwhile, the optimum period of fermentation was 100 h, which gave the yield of lactic acid production of 22.30 g. Lactobacillus casei was considered to have lower ability to effectively produce lactic acid from sugar palm sap compared to Lactobacillus plantarum

L-Lactic Acid Fermentation by Rhizopus oryzae Using Dairy Manure as a Nitrogen Source

2009 ◽  
Vol 52 (6) ◽  
pp. 2047-2054 ◽  
Author(s):  
W. Yao ◽  
X. Wu ◽  
J. Zhu ◽  
B. Sun ◽  
C. Miller
Keyword(s):  
Lactic Acid ◽  
Nitrogen Source ◽  
Rhizopus Oryzae ◽  
Dairy Manure ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation

scholarly journals Utilization of digested sewage sludge in lactic acid fermentation

Detritus ◽  
2021 ◽  
pp. 48-53
Author(s):  
Daniel Pleissner ◽  
Clemens Krieg ◽  
Jan Christoph Peinemann
Keyword(s):  
Lactic Acid ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Nitrogen Source ◽  
Sulphuric Acid ◽  
Organic Nitrogen ◽  
Lactic Acid Fermentation ◽  
Nitrogen Compounds ◽  
Acid Fermentation ◽  
Digested Sewage Sludge

The management of sewage sludge is mostly limited to anaerobic digestion, incineration of digestate and recovery of phosphorous. In terms of resource efficiency, it is recommended to make use of the potential of all organic compounds. Nitrogen compounds, for instance, can find application as nutrients in biotechnological processes. To follow this approach, sewage sludge collected after anaerobic digestion, which had carbon and nitrogen contents of 35.9% (w/w) and 5.6% (w/w), respectively, was first hydrolyzed using 0-1% (w/w) sulphuric acid for 15 minutes at 121°C and the hydrolysate used as nitrogen source in lactic acid fermentation. Even though the focus was on a recovery of nitrogen compounds, the hydrolytic treatment with 1% (v/v) sulphuric acid resulted in a release of 28 mg g-1 glucose. Because of the complex composition of the obtained hydrolysate it was not possible to quantify the released organic nitrogen compounds. Lactic acid fermentations, however, revealed that the concentration of organic nitrogen compounds was sufficient to efficiently convert 10 g L-1 of added glucose into 9 g L-1 lactic acid, and thus it is expected that digested sewage sludge may be an alternative nitrogen source in lactic acid fermentation, possibly combined with the utilization of a carbon-rich feedstock. Such a utilization approach goes beyond the conventional management strategies of digestated sewage sludge and allows a material utilization even after anaerobic digestion.

Conversion of hydrophilic constituents from Mercurialis perennis L. by lactic acid fermentation

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
P Lorenz ◽  
S Duckstein ◽  
J Bertrams ◽  
U Meyer ◽  
F Stintzing
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation
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