Aim: to evaluate the efficacy of non-pharmacological sedation method with concentrate glucose solution during the hearing test in infants of the first 3 months of life. Patients and Methods: a non-randomized clinical study on the efficacy of non-pharmacological sedation method with 25% glucose solution was performed during the hearing tests (tympanometry and otoacoustic emission) in infants from birth to 3 months of life versus breastfeeding in three parallel groups. The first group included infants examined with the proposed sedation method with 25% glucose solution. The second group consisted of patients who underwent the hearing test within an hour after feeding (n=30). The third group included infants examined an hour or more after feeding who didn’t underwent the sedation (n=24). To evaluate the efficacy of the proposed method, we used the Newborn Acute Pain (DAN) scale. Results: in the infant group receiving 25% glucose solution during the test, the DAN score was 1 (Q25; Q75 (0; 2)) point; in infants examined after feeding — 2 (2; 4) points, and in the third infant group — 4 (4; 5) points. The Kruskal-Wallis test showed statistically significant differences between patient groups (p<0.0001). Subsequent post hoc analysis established the significance of differences in infant behavior between groups where sedation with glucose was and wasn’t performed (p<0.0001), as well as between infants examined after feeding and after sedation with glucose (p<0.0001). Conclusions: the use of 25% glucose solution reduces the discomfort reaction in infants of the first 3 months of life when conducting the hearing test and is more effective than breastfeeding. The hearing test of infants in this age group should be performed within the first hour after feeding. KEYWORDS: hearing test, hearing loss, infants, glucose, non-pharmacological sedation, endogenous opiates. FOR CITATION: Merkulova E.P., Ustinovich K.N. Hearing tests of infants using the non-pharmacological sedation method. Russian Medical Inquiry. 2020;4(4):233–237. DOI: 10.32364/2587-6821-2020-4-4-233-237.