Triticum durum Desf. selection 56-1 (2n = 28,AABB) genomes were introduced into the cytoplasms of six species of Triticum, 14 of Aegilops, and one each of Secale and Haynaldia by the backcross method. Of the 22 alloplasmic lines, 14 were completely male sterile, four were partially fertile, and the remaining four, having cytoplasms of T. dicoccoides Körn, Ae. kotschyi Boiss., Ae. variabilis, Eig. or H. villosa L., were of normal fertility. Eleven of these lines headed late and 14 had lower plant height than the control euplasmic T. durum. The 14 male-sterile lines were crossed with five R-lines derived from crosses involving T. nudiglumis Nabalek and T. durum, and male-fertility restoration in F1 hybrids was examined. All five R-lines restored male fertility to six of the male-sterile lines having cytoplasms of Ae. speltoides Tausch., Ae. bicornis Forsk., T. nudiglumis, T. araraticum Jakubz., T. timopheevi Zhuk., or T. zhukovskyi Men. &Er. The male fertility of the alloplasmic line having rye (secale cereale L.) cytoplasm was completely restored by RE 5 and partially by RE 2. Also, RE 5 restored plant vigor to durum plants having rye cytoplasm. None of the five R-lines restored male fertility or plant vigor to any of the six male-sterile lines having cytoplasms of T. boeoticum Boiss., Ae. caudata L., Ae. umbellulata Zhuk., Ae. heldreichii Holzm., Ae. sharonensis Eig., or Ae. triaristata Willd. In general, F2 data from crosses involving four of the male-sterile lines and five R-lines indicated that male fertility restoration was simply inherited.