Eric Vaughn snRNASeq Protocol v1

2021 ◽  
Author(s):  
Eric Vaughn ◽  
Catherine Dulac
Keyword(s):  
Free Water ◽  
Rnase Inhibitor ◽  
Lysis Buffer

This protocol is designed to extract single nuclei for sequencing with 10X Genomics technologies. Note: For each step, keep tissue/nuclei/reagents/centrifuge on ice/cold to reduce IEG expression and maintain nuclear integrity. Buffers to prepare: -100mL Lysis Buffer Store @ 4 C: 98.4mL Nuclease-free water 1mL 1M Tris-HCl (10mM) 200uL 5M NaCl (10mM) 300uL 1M MgCl2 (3mM) 100uL NonIdet P40 (0.1%) -PBS w/ 1% BSA and RNAse inhibitor (new batch for each run; enough for 3-4 separate samples): 10mL PBS 100 mg powdered BSA (mix well) 50uL Promega RNasin (40U/uL; final conc: 0.2U/uL)

Microwave resonance method for measuring microliter volumes of free moisture in aviation fuels

2020 ◽  
pp. 49-56
Author(s):  
Vitaly V. Volkov ◽  
Michael A. Suslin ◽  
Jamil U. Dumbolov
Keyword(s):  
Solid Surface ◽  
Free Water ◽  
Resonance Method ◽  
Cavity Resonator ◽  
Maximum Error ◽  
Aviation Fuel ◽  
Fuel Quality ◽  
Microwave Resonance ◽  
Free Moisture ◽  
Microwave Losses

One of the conditions for ensuring the safety of air transport operation is the quality of aviation fuel refueled in aircraft. Fuel quality control is a multi-parameter task that includes monitoring the free moisture content. Regulatory documents establish the content of free water no more than 0.0015% by weight. It is developed a direct electrometric microwave resonance method for controlling free moisture in aviation fuels, which consists in changing the shape of the water drops by pressing them on a solid surface inside a cylindrical cavity resonator. This can dramatically increase dielectric losses. Analytical and experimental analysis of the proposed method is carried out. The control range from 0,5 to 30 μl of absolute volume of moisture in aviation fuels with a maximum error of not morethan 25 % is justified. The sensitivity of the proposed method for monitoring microwave losses in free moisture drops transformed into a thin layer by pressing is an order of magnitude greater than the sensitivity of the method for monitoring microwave losses in moisture drops on a solid surface in a resonator. The proposed method can be used as a basis for the development of devices for monitoring the free moisture of aviation fuels in the conditions of the airfield and laboratory. The direction of development of the method is shown.

EFEKTIVITAS TANAMAN GENJER DALAM MENURUNKAN BOD DAN COD LIMBAH CAIR TAHU HASIL PROSES ANAEROB

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Muhammad Irvan Nurliansyah
Keyword(s):  
Water Surface ◽  
Free Water ◽  
Surface Flow ◽  
Free Water Surface

ABSTRAK Limbah cair tahu merupakan limbah cair yang berasal dari proses pembuatan tahu. Limbah cair tahu mengandung senyawa organik yang tinggi. Pembuangan limbah cair tahu secara langsung ke badan air tanpa dilakukan pengolahan dapat mempengaruhi dan mencemari lingkungan. Suatu cara untuk menanggulangi permasalahan tersebut adalah melakukan pengolahan limbah cair tahu. Salah satu alternatif pengolahan limbah cair tahu yang dapat digunakan adalah fitoremediasi menggunakan tanaman genjer. Penelitian ini bertujuan untuk mengetahui efisiensi pengolahan dan efektivitas waktu tinggal pengolahan limbah cair tahu menggunakan tanaman genjer dalam menurunkan BOD dan COD effluen hasil proses pengolahan anaerob limbah cair tahu. Metode yang digunakan dalam penelitian ini adalah fitoremediasi menggunakan tanaman genjer pada sistem lahan basah buatan Free Water Surface flow dengan waktu tinggal 3 hari, 5 hari dan 7 hari. Hasil penelitian menunjukkan bahwa efisiensi pengolahan secara fitoremediasi pada hari ke 3 untuk BOD dan COD berturut-turut sebesar 21,28% dan 16,13%. Pada hari ke 5 efisiensi pengolahan yang diperoleh untuk BOD dan COD berturut-turut sebesar 52,60% dan 45,93% sedangkan efisiensi pengolahan pada hari ke 7 untuk BOD dan COD berturut-turut sebesar 76,42% dan 70,74%. Waktu tinggal efektif yang diperoleh pada penelitian ini adalah  7 hari dengan nilai BOD dan COD telah berada dibawah baku mutu yaitu berturut-turut sebesar 72,72 mg/l dan 213,33 mg/l.   Kata kunci : limbah cair tahu, fitoremediasi, tanaman genjer, efisiensi pengolahan, waktu tinggal

Drawdowns at the Free Water Table During Pumping Tests in Artesian and Semiartesian Aquifers

1980 ◽  
Vol 11 (3-4) ◽  
pp. 159-168 ◽  
Author(s):  
Henrik Kærgaard
Keyword(s):  
Water Table ◽  
Free Water ◽  
Pumping Test ◽  
Water Withdrawal ◽  
Specific Yield ◽  
Minor Role ◽  
Long Term Effects ◽  
Long Term Storage ◽  
Term Storage

In an earlier paper I have shown an example of how long term drawdowns can be used for the computation of long term storage in artesian and semiartesian areas. In most cases the long term storage is more or less equivalent to the specific yield at the water table; the storage mechanisms of consolidation playing a minor role in long term situations. The specific yield in artesian areas is a very important parameter in the prediction of long term effects of ground water withdrawal. Especially the stream depletion will often mainly be governed by draw-downs in upper nonpumped aquifers near the water table, and these drawdowns depend to a great extent on the specific yield at the water table. A determination of long term storage will often necessitate long term draw-down data, however, under certain circumstances a determination can be made on the basis of a pumping test of limited duration (3-5 weeks) provided drawdown observations at the water table can be made. In this paper some formulas dealing with water table drawdowns in different geohydrologic systems are reviewed, and two cases in which these formulas have been used in practice are presented.

Remote turbidity measurement with a laser reflectometer

1998 ◽  
Vol 37 (12) ◽  
pp. 255-261 ◽  
Author(s):  
Mark Johnson
Keyword(s):  
Water Surface ◽  
Free Water ◽  
Optical Scattering ◽  
Flowing Water ◽  
Water Stream ◽  
Water Turbidity ◽  
Water Colour ◽  
Contact Measurement ◽  
Optical Instruments ◽  
Better Than

A simple, laser-based reflectometer is described for the measurement of water turbidity via 180° optical scattering. Applications exist both in clean source waters (0-1000NTU) with a minimum detectable turbidity better than 1NTU, and in dense wastewater primary-clarifier sludges. The non-contact measurement is performed from a distance at least up to 10m, substantially avoiding the usual window fouling problems of optical instruments. By measuring directly in the process, through a free water surface or on the side of a flowing water stream, the difficulties of transporting sample to the instrument are also avoided. Extensions to be described allow measurement also of water colour.

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