In order to study the stability of Montmorillonite / Octadecyl trimethyl ammonium complexes in engineering application, the influences of the organic modifier dosage, oscillation intensity, oscillation time, temperature, pH, sodium salt concentration, calcium salt concentration on the stability of Montmorillonite / Octadecyl trimethyl ammonium complexes were systematically investigated. Research results show that the stability of Montmorillonite / Octadecyl trimethyl ammonium complexes slightly reduces with the increases of oscillation intensity, oscillation time and temperature, but the impact isn’t obvious. The more the organic modifier dosage is, the more unstable the Montmorillonite / Octadecyl trimethyl ammonium complexes are. The stability of the Montmorillonite / Octadecyl trimethyl ammonium complexes decreases significantly under the medium conditions of lower or higher pH. The higher the concentration of sodium salts is, the more stable the Montmorillonite / Octadecyl trimethyl ammonium complexes are, but calcium salts are on the contrary.