Preliminary Phytochemical Analysis: In-Vitro Comparative Evaluation of Anti-arthritic and Anti-inflammatory Potential of Some Traditionally Used Medicinal Plants

Background: Colchicum autumnale, Strychnous nux-vomica and Aloe barbadensis are the medicinal plants clinically utilized for the management of rhuematic disorders. Purpose: The present work was focused to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of Colchicum ( Colchicum autumnale), Nux-vomica ( Strychnous nux-vomica), and Aloe-vera ( Aloe barbadensis). Research Design: Primarily, the aqueous-ethanolic extracts of these medicinal plants were phytochemically screened followed by Fourier Transform Infrared (FTIR) analysis. Anti-arthritic activity by protein denaturation method and anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization method at the concentration of 125, 250, and 500 µg/mL along with standard were performed. Results: Phytochemical screening revealed that alkaloids, saponins, terpenoids, phenols, and anthraquinones were found in all the extracts, and organic acids, amine group, aromatic or aliphatic compounds, esters and halogens, and phenolics were identified by FTIR. Protein denaturation method revealed that colchicum, nux-vomica, and aloe-vera showed maximum 98.5%, 99.6%, and 72.3% of inhibition at 500 µg/mL compared with that of standard drug, that is, Diclofenac sodium. Membrane stabilization method showed that colchicum, nux-vomica, and aloe-vera showed maximum 40.20%, 35.67%, and 40.1% protection at 500 µg/mL when compared with standard drug. Conclusion: It is concluded from the current study that extracts of colchicum, nux-vomica, and aloe-vera showed more potent effect and thus can be used as alternative options for the management of inflammatory and arthritic ailments.

Nowe stanowiska roślin naczyniowych Polski, 2

Streszczenie W pracy przedstawiono nowe stanowiska 12 rzadkich (lub regionalnie rzadkich) roślin naczyniowych w Polsce, tj. Cochlearia danica , Colchicum autumnale , Cuscuta campestris , Lythrum hyssopifolia , Ostericum palustre , Pedicularis sylvatica , Plantago coronopus , Ranunculus arvensis , Rosa gallica , Sesleria uliginosa , Succisella inflexa oraz Veronica prostrata .

Several features of growing Colchicum autumnale l. in the urban environment of Tashkent-Uzbekistan

For the first time, the collection of autumn colchicum was created at the experimental field site of the Tashkent State Agrarian University. In this paper, the issues of growing conditions for the growth and development of Colchicum autumnale l plant were deeply studied and investigated. It was revealed that the Colchicum autumnale l growth and development largely depend on the size of the corm and the type of soil conditions.

ОСЕРЕДКИ РІДКІСНИХ ВИДІВ В ЛУЧНО-ПАСОВИЩНИХ ТА ЛІСОВИХ ОСЕ-ЛИЩАХ ГОРИ ГВІЗД (СКИБОВІ ГОРГАНИ)

В статті подано характеристику ценопопуляцій рідкісних видів: Colchicum autumnale L., Gladiolus imbricatus L., Dactylorhiza majalis (Reichenb.) P. F. Hunt et Summerhayes, D. sambucina (L.) Soо, Platanthera bifolia (L.) Rich., Gymnadenia conopsea (L.) R. Br., Listera ovata (L.) R. Вr., які зростають на лучних схилах гори Гвізд – на межі Богород-чанського та Надвірнянського районів Івано-Франківської області.

Herbstzeitlose, Colchicum autumnale L.

Zusammenfassung Colchicum autumnale L., die Herbstzeitlose, ist eine in Mitteleuropa verbreitete Pflanze, die v. a. Tropolonalkaloide wie Colchicin, Demecolcin sowie Colchicosid enthält. Der Hauptwirkstoff Colchicin verhindert die Ausbildung des zellulären Mikrotubuli-Apparates, was mit einer Entzündungshemmung verbunden ist. Trotz hoher Toxizität werden isoliertes Colchicin und Herbstzeitlosen-Extrakte zur Behandlung von Gicht eingesetzt. Weitere Anwendungsgebiete werden derzeit erforscht.

DIVERZITET I ANALIZA KVALITATIVNOG SASTAVA BILJNE ZAJEDNICE STRNIŠTA KAO POTENCIJALNE KRME

In the plant communities on stubble, 25 taxons were determined, classified into 22 genera and 13 families. Of the 7 most pre-stated families, five are present in this micro-company where asteraceae have 6 taxons (27.27%), Lamiaceae five species (20%) and Poaceae three representatives (12%). As feed can be used: Galinsoga parviflora Cav., Echinochloa crus-galli (L.) Beauv., Elymus repens (L.) Gould., Amaranthus retroflexus L., Chenopodium album L. and Leontodon hispidus L. Potentially toxic to domestic animals are: Colchicum autumnale L., Equisetum arvense L., Equisetum telmateia Ehrh., Ranunculus bulbosus L., Sonhus arvensis L.

About polemohoral populations of Colchicum autumnale

В Тверской области обнаружены полемохорные популяции Colchicum autumnale L. Охарактеризована специфика местообитаний вида. Рассмотрены особенности его биологии и экологии. We found polemochoral populations of Colchicum autumnale L. in the Tver Region. We describe specific habitats along with the features of the biology and ecology of the mentioned species.