Typification of 20 names in Orchidaceae of the Russian flora

The article represents the typification of 20 names of orchids from the Flora of Russia. The typified names in the current use are the following: Goodyera henryi, Orchis comperiana (basionym for Himantoglossum comperianum), Orchis salina (basionym for Dactylorhiza salina), and Listera nipponica (basionym for Neottia nipponica). Other typified names represent synonyms, which are assertained by the types provided here: Calypso japonica, Gymnadenia conopsea var. alpina, G. conopsea var. ussuriensis, G. pauciflora, Listera eschscholziana, L. yatabei, Malaxis diphyllos, Neottia nidus-avis var. manshurica, Orchis incarnata var. knorringiana, O. monticola subsp. caucasica, O. orientalis subsp. turcestanica, O. punctulata var. sepulchralis, O. salina f. elatior, O. taurica, O. viridifusca, Platanthera tipuloides var. sibirica.

ОСЕРЕДКИ РІДКІСНИХ ВИДІВ В ЛУЧНО-ПАСОВИЩНИХ ТА ЛІСОВИХ ОСЕ-ЛИЩАХ ГОРИ ГВІЗД (СКИБОВІ ГОРГАНИ)

В статті подано характеристику ценопопуляцій рідкісних видів: Colchicum autumnale L., Gladiolus imbricatus L., Dactylorhiza majalis (Reichenb.) P. F. Hunt et Summerhayes, D. sambucina (L.) Soо, Platanthera bifolia (L.) Rich., Gymnadenia conopsea (L.) R. Br., Listera ovata (L.) R. Вr., які зростають на лучних схилах гори Гвізд – на межі Богород-чанського та Надвірнянського районів Івано-Франківської області.

The Diversity Analysis and Functional Prediction of Endophytic Fungi Community of Gymnadenia Conopsea from Different Altitudes in Tibet

Gymnadenia conopsea has high economic value, and can be used as a medicinal and ornamental plant. Due to its low natural reproduction rate and overexploitation, the extinction of this plant is gradually accelerating. Understanding the composition and diversity of endophytic fungi is of great significance in promoting its propagation and the utilization of beneficial fungal strains. In this study, the diversity of fungal communities from roots, stems, leaves, fruits, and soils at four different elevations was studied with Illumina MiSeq sequencing. A total of 3,707,871 sequences were detected from all samples, and the number of clustering OTUs was 14,800. The OTUs were assigned to 4 phyla, 17 classes, 41 orders, 73 families, and 99 genera. The predominant fungal groups included Ascomycota and Basidiomycota, accounting for 33.71%-86.38% and 6.98%-58.30% of the total species, respectively. According to the alpha diversity index analysis, the diversity and richness of endophytic fungal communities in plant tissues at low altitudes were higher than those at high altitudes, while the diversity and richness of soil fungi were the opposite. In addition, principal coordinate analysis (PCoA) not only showed that the fungal community structure was correlated with altitude but also indicated tissue specificity of the community structure. Our study explored the composition of the endophytic fungal community among different tissues from different altitudes and included functional analysis, which might provide new ideas for saving the endangered species G. conopsea.

Variability of Population and Individual Characters of Gymnadenia conopsea (L.) R. Br. (Orchidaceae, Liliopsida) under Anthropogenic Load Conditions in Meadow Ecosystems of Central Caucasus

We studied the variability and phytocenotic plasticity of the morphological characters of individuals, and the numbers, density, ontogenetic structure and vitality of 11 coenopopulations of Gymnadenia conopsea (L.) R. Br. within the Kabardino-Balkar Republic in 2015–2019. The variability and plasticity of plants features provide their adaptation to various growing conditions and improve the morphological heterogeneity of coenopopulations. The dynamics of abundance, density, age structure and vitality of coenopopulations in the changing conditions of the environment provides information about life strategies of the species. Age conditions, the variability and plasticity of plants were studied on the basis of morphological parameters of the aboveground organs without digging the plants. The studies were conducted in meadow plant communities under various conditions of anthropogenic load (recreation and grazing). The species is characterized by medium-high phytocenotic plasticity of morphological characters (Ip = 38–68%) and high variability of morphological characters (CVх¯ср = 17.10– 33.63%) under changeable growth conditions. Intensification of growth processes in the individuals with the maximum increase of the coenopopulation vitality (IVC = 0.98– 1.16) was observed in the composition of undisturbed highly wet meadows; the portion of generative individuals increased in their ontogenetic spectra (48.6–56.5%). Under the anthropogenic load intensification, the vitality of coenopopulations (IVC = 0.82– 0.95) and the density of individuals decreased; the portion of virginal individuals increased in their ontogenetic spectra (rubbly meadows) or coenopopulations were clearly aging. The rate of the seed reproduction of coenopopulations, where the density of individuals accounted for 16 ind./m2 , was high on rubbly substrata with low total projective cover (60%). Our studies have identified the mixed type of life strategy for Gymnadenia conopsea with stress-tolerant and ruderal components. The ruderal-stress-tolerant life strategy allows the species to grow for a long time in conditions of high interspecific competition and anthropogenic load, spreading quickly to unoccupied territories.

Genome size variability of the population of Gymnadenia conopsea (Orchidaceae) in the Novosibirsk Region (Russia)

Gymnadenia conopsea (Fragrant orchid) is a widespread model species for the study of polymorphism in the European part of Russia and abroad. Flow cytometry refers to rapid methods effective for assessing the structure of a population by DNA size estimation. However, this method is only available in habitats with close proximity to the place of the experiment, because this indicator is determined only in fresh leaves. A small population of G. conopsea in the industrial area adjacent to the Novosibirsk urban agglomeration in the Novosibirsk Region (NR) was studied. Only diploids were detected in the G. conopsea cenopopulation occurred in the industrial zone of the NR. In this study we pointed on significance of genome size variation, which correlates with the adaptive evolution of the G. conopsea in West Siberia. The revealed absence of minor cytotypes (individuals with a higher level of ploidy) in G. conopsea population reduces intraspecific and intrapopulation diversity of the species.

Prirodna obilježja Svilaje s pregledom istraživanja flore i vegetacije

Svilaja (1508 m n. v.) je planina u Dalmatinskoj Zagori, u središnjem dijelu unutrašnje Dalmacije. Flora i vegetacija planine Svilaje do sada nije sustavno istraživana. Od 2013. obavljaju se opsežna terenska istraživanja s ciljem inventarizacije ukupne flore. U ovom radu su, na temelju dostupnih podataka iz literature, prikazana obilježja reljefa, klime i fitogeografski položaj s općim pregledom vegetacije. U glavnom dijelu rada prikazan je detaljni pregled dosadašnjih istraživanja flore i vegetacije Svilaje. Analizirani su samo oni literaturni podaci o nalazima biljnih svojti i zajednica čija su nalazišta (lokaliteti) unutar granica istraživanog područja. Prema analiziranim literaturnim podacima, do sada je za područje Svilaje zabilježeno približno 700 svojti vaskularne flore, među kojima je značajan broj endemičnih, ugroženih i zaštićenih svojti kao npr.: Arenaria orbicularis, Astragalus monspessulanus ssp. illyricus, Crocus biflorus ssp. weldenii, Dianthus sylvestris ssp. tergestinus, Edraianthus tenuifolius, Hyacinthella dalmatica, Pulsatilla grandis, Thymus bracteosus, itd. Prema dosadašnjim saznanjima Svilaja se odlikuje iznimno bogatom florom orhideja (Orchidaceae): Coeloglossum viride, Dactylorhiza maculata, Gymnadenia conopsea, Listera ovata, Ophrys apifera, O. dinarica, O. tetraloniae, Orchis militaris, O. morio, O. tridentata, Platanthera chlorantha, itd. Točan broj prethodno zabilježenih svojti i biljnih zajednica nije moguće preciznije utvrditi zbog razlika u shvaćanju granica Svilaje i nepreciznom navođenju lokaliteta u prethodno objavljenim radovima. Podaci o dosadašnjim istraživanjima neophodno su polazište za sustavna istraživanja flore Svilaje koja su u tijeku. Na neophodnost sustavnih istraživanja flore Svilaje ukazuje starost većine zabilježenih nalaza i značajne promjene u biljnom pokrovu uzrokovane snažnom depopulacijom i smanjivanjem intenziteta tradicionalnog stočarstva i poljodjelstva te brojnim požarima.