Development of a novel 68Ga-dextran carboxylate derivative for blood pool imaging

To develop a possible PET blood pool imaging agent, a series 68Ga-dextran carboxylate derivatives were prepared. Dextran carboxylates with different degree of oxidations (DO) were prepared through stepwise dextran oxidation using NaIO4 and CH3COOOH. The products were characterized by FT-IR and GPC, followed by solubility and toxicity tests on Hella cells (viability=98.6, 97.4 and 95.6% for 3 dextran carboxylates with DOs: 8.3, 24.6 and 39.8%, respectively. The products were labeled with 68Ga (radiochemical purity>98%; ITLC) followed by stability tests in final solution as well as in presence of cycteine and human serum. Two stable tracers (DOs; 24.6 and 39.8%) were adminstered intravenously into wild type rat tail vein separately demonstrating suitable retention in circulation as expected from blood pool imaging agents. Liver and spleen also contained activities. The major excretion was through urinary pathway esp. for derivative with DO. 39.8%. Unlike 68Ga-dextran, lungs showed lower uptake. The dextran carboxylate with the highest 39.8% showed the best characteristics for a blood pool agent, though more studies including PET imaging in larger mammals are required.

HYBRID SCINTIGRAPHY OF RARE SPLENIC HAMARTOMA

Splenic hamartoma, also known as splenoma, splenic adenoma or nodular hyperplasia, is a rare benign splenic tumor. It is composed of disordered vascular channels with abnormal mixture of red or white splenic pulp. Mostly it is asymptomatic and encountered incidentally at imaging, surgery or autopsy. Radiologically hamartomas are almost identical to hemangioma. In such circumstances radiolabelled blood pool imaging and radiocolloid scintigraphy helps to differentiate the two entities. We present a case of asymptomatic splenic lesion on hybrid radionuclide imaging with SPECT-CT, findings suggestive of hamartoma. Key Words: Splenic hamartoma, SPECT-CT, Radiocolloid scintigraphy

Automated synthesis of [68Ga]oxine, improved preparation of68Ga-labeled erythrocytes for blood-pool imaging, and preclinical evaluation in rodents

Radiolabeled erythrocytes have multiple applications in nuclear medicine, including blood pool imaging.