mesorhizobium huakuii
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2020 ◽  
Author(s):  
Hetao Wu ◽  
Qian Zou ◽  
Sha Luo ◽  
Donglan He ◽  
Xiaohua Li ◽  
...  

Abstract Background: Flavin-containing monooxygenases (FMOs) catalyze the NADPH-dependent N- or S- oxygenation of numerous foreign chemicals, and thus may mediate interactions between microorganisms and their chemical environment. The aim of this study was to investigate the role of FMO in symbiotic nitrogen fixation of Mesorhizobium huakuii and its host plant Astragalus sinicus.Results: A mutation in M. huakuii fmoA gene was generated by homologous recombination. The fmoA mutant grew more slowly than its parental strain, and displayed decreased antioxidative capacity under higher concentration of H2O2 and cumene hydroperoxide (CUOOH), indicating that FmoA plays an important role in response to the peroxides. The fmoA mutant strain displayed no difference of peroxidase activity and glutathione reductase activity, but significantly lower level of glutathione and hydrogen peroxide content than the wild type. Real-time quantitative PCR showed that the fmoA gene expression is significantly up-regulated in three different stages of nodule development. The fmoA mutant was severely impaired in its rhizosphere colonization, and its symbiotic properties in Astragalus sinicus were drastically affected. Transcriptomes in root-nodule bacteroids were analyzed and compared. A total of 1233 genes were differentially expressed, of which 560 were up-regulated and 673 were down-regulated in HKfmoA bacteroids compared with that in 7653R bacteroids. The transcriptomic data allowed us to determine additional target genes, whose differential expression was able to explain the observed the changes of symbiotic phenotype in the mutant-infected nodules.Conclusions: The fmoA gene is essential for antioxidant capacity and symbiotic nitrogen fixation. Furthermore, RNA-Seq based global transcriptomic analysis provided a comprehensive view of M. huakuii fmoA gene involved in nodule senescence and symbiotic nitrogen fixation.



2019 ◽  
Vol 77 (1) ◽  
pp. 1-10
Author(s):  
Sha Luo ◽  
Jie Yin ◽  
Yang Peng ◽  
Jing Xie ◽  
Hetao Wu ◽  
...  


2019 ◽  
Vol 8 (10) ◽  
Author(s):  
Sanjiao Wang ◽  
Tiantian Lu ◽  
Qiang Xue ◽  
Ke Xu ◽  
Guojun Cheng


2019 ◽  
Vol 180 (1) ◽  
pp. 509-528
Author(s):  
Donglai Zhou ◽  
Yanan Li ◽  
Xuting Wang ◽  
Fuli Xie ◽  
Dasong Chen ◽  
...  


2017 ◽  
Vol 8 ◽  
Author(s):  
Xie Fuli ◽  
Zhao Wenlong ◽  
Wang Xiao ◽  
Zhang Jing ◽  
Hao Baohai ◽  
...  


PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e93626 ◽  
Author(s):  
Jieli Peng ◽  
Baohai Hao ◽  
Liu Liu ◽  
Shanming Wang ◽  
Binguang Ma ◽  
...  


BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 440 ◽  
Author(s):  
Shanming Wang ◽  
Baohai Hao ◽  
Jiarui Li ◽  
Huilin Gu ◽  
Jieli Peng ◽  
...  


PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47677 ◽  
Author(s):  
Heng Wee Tan ◽  
Bevan S. Weir ◽  
Noel Carter ◽  
Peter B. Heenan ◽  
Hayley J. Ridgway ◽  
...  


2011 ◽  
Vol 77 (15) ◽  
pp. 5513-5516 ◽  
Author(s):  
Osei Yaw Ampomah ◽  
Kerstin Huss-Danell

ABSTRACTVery little is known about rhizobia that form nodules onThermopsisspp. We report the isolation of aMesorhizobium huakuiistrain with a uniquenodAgene that form nodules onThermopsis lupinoidesin Kamtchatka, Russia. The isolate did not form nodules onThermopsis chinensisorThermopsis caroliniana, which suggests it may be host specific.



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