carbohydrate arrays
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2022 ◽  
Vol 18 (1) ◽  
pp. e1010226
Author(s):  
Carolina De Marco Verissimo ◽  
Heather L. Jewhurst ◽  
József Dobó ◽  
Péter Gál ◽  
John P. Dalton ◽  
...  

The complement system is a first-line innate host immune defence against invading pathogens. It is activated via three pathways, termed Classical, Lectin and Alternative, which are mediated by antibodies, carbohydrate arrays or microbial liposaccharides, respectively. The three complement pathways converge in the formation of C3-convertase followed by the assembly of a lethal pore-like structure, the membrane attack complex (MAC), on the pathogen surface. We found that the infectious stage of the helminth parasite Fasciola hepatica, the newly excysted juvenile (NEJ), is resistant to the damaging effects of complement. Despite being coated with mannosylated proteins, the main initiator of the Lectin pathway, the mannose binding lectin (MBL), does not bind to the surface of live NEJ. In addition, we found that recombinantly expressed serine protease inhibitors secreted by NEJ (rFhSrp1 and rFhSrp2) selectively prevent activation of the complement via the Lectin pathway. Our experiments demonstrate that rFhSrp1 and rFhSrp2 inhibit native and recombinant MBL-associated serine proteases (MASPs), impairing the primary step that mediates C3b and C4b deposition on the NEJ surface. Indeed, immunofluorescence studies show that MBL, C3b, C4b or MAC are not deposited on the surface of NEJ incubated in normal human serum. Taken together, our findings uncover new means by which a helminth parasite prevents the activation of the Lectin complement pathway to become refractory to killing via this host response, in spite of presenting an assortment of glycans on their surface.


2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jolanda M. van Munster ◽  
Baptiste Thomas ◽  
Michel Riese ◽  
Adrienne L. Davis ◽  
Christopher J. Gray ◽  
...  

2015 ◽  
Vol 3 (1) ◽  
pp. 175-181 ◽  
Author(s):  
Caroline I. Biggs ◽  
Steve Edmondson ◽  
Matthew I. Gibson

Carbohydrate arrays are a vital tool in studying infection, probing the mechanisms of bacterial, viral and toxin adhesion and the development of new treatments, by mimicking the structure of the glycocalyx.


ChemBioChem ◽  
2014 ◽  
Vol 15 (16) ◽  
pp. 2450-2457 ◽  
Author(s):  
Dorith Claes ◽  
Elisabeth Memmel ◽  
Malte Holzapfel ◽  
Jürgen Seibel ◽  
Wolfgang Maison

2013 ◽  
Vol 21 (3) ◽  
pp. 288-295 ◽  
Author(s):  
Gangliang Huang ◽  
Xin Chen ◽  
Feng Xiao
Keyword(s):  

2012 ◽  
Vol 8 (9) ◽  
pp. 769-773 ◽  
Author(s):  
Lan Ban ◽  
Nicholas Pettit ◽  
Lei Li ◽  
Andreea D Stuparu ◽  
Li Cai ◽  
...  

2012 ◽  
Vol 18 (21) ◽  
pp. 6548-6554 ◽  
Author(s):  
Henning S. G. Beckmann ◽  
Andrea Niederwieser ◽  
Manfred Wiessler ◽  
Valentin Wittmann

2010 ◽  
Vol 16 (3) ◽  
pp. 131-137 ◽  
Author(s):  
Nades Palaniyar

Soluble pattern-recognition innate immune proteins functionally resemble the antibodies of the adaptive immune system. Two major families of such proteins are ficolins and collectins or collagenous lectins (e.g. mannose-binding lectin [MBL], surfactant proteins [SP-A and SP-D] and conglutinin). In general, subunits of ficolins and collectins recognize the carbohydrate arrays of their targets via globular trimeric carbohydrate-recognition domains (CRDs) whereas IgG, IgM and other antibody isotypes recognize proteins via dimeric antigen-binding domains (Fab). Considering the structure and functions of these proteins, ficolins and MBL are analogous to molecules with the complement activating functions of C1q and the target recognition ability of IgG. Although the structure of SP-A is similar to MBL, it does not activate the complement system. Surfactant protein-D and conglutinin could be considered as the collagenous non-complement activating giant IgMs of the innate immune system. Proteins such as peptidoglycan-recognition proteins, pentraxins and agglutinin gp-340/DMBT1 are also pattern-recognition proteins. These proteins may be considered as different isotypes of antibody-like molecules. Proteins such as defensins, cathelicidins and lactoferrins directly or indirectly alter microbes or microbial growth. These proteins may not be considered as antibodies of the innate immune system. Hence, ficolins and collectins could be considered as specialized ‘antibodies of the innate immune system’ instead of ‘ante-antibody’ innate immune molecules. The discovery, structure, functions and future research directions of many of these soluble proteins and receptors such as Toll-like and NOD-like receptors are discussed in this special issue of Innate Immunity.


ChemInform ◽  
2010 ◽  
Vol 33 (52) ◽  
pp. no-no
Author(s):  
Kerry R. Love ◽  
Peter H. Seeberger
Keyword(s):  

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