In recent years, over 75% civil aviation accidents are caused by pilots manipulation mistake or cognitive error, but there is still no quantitative method to research pilots error. With the statistics and analysis of NTSB (National Transportation Safety Board) aviation database, the aviation case base ontology model has been established in the paper, which was the closest airworthiness directive to classify the aviation accidents according to the classification of aviation system. The pilots error in the aviation accident and the factors possibly causing the pilots error were analyzed based on Reason model to propose the inducing factors for pilots error. With the human factor analytical method, the behavior factors inducing the pilots error obtained from the pilot himself included excessive manipulation number, external environment interference, insufficient experience, physiological and psychological conditions, insufficient time margin. Pilots behavior shaping factors can guide the rational design of manipulation procedure and pilot training, thus enhancing the flight safety.