An investigation was conducted on the number and morphology of the chromosomes of four species from the family <em>Commelinacene</em>:<em> Spironema fragrans </em>Lindl., <em>Setereasea purpurea </em>Boom, <em>Tradesca</em><em>nt</em><em>ia albiflora </em>Kunth. and <em>Tradescantia blossfeldiana </em>Mildbr. It was found that the karyotype of <em>Spironema fragrans </em>Lindl. was 2n = 12 chromosomes whose total length was 6.9-13.7 µm and the formula of the karyotype was: 2m+2sm +2m+2t+2st<sup>sat</sup> +2st <em>Setcreasea purpurea </em>Boom. had 2n = 24 chromosomes, 9.1-12.1 µm in length and the centromere positioned as represented by the formula 24m. The karyotype of <em>Tradsescantia albiflora </em>Kunth. had 2n = 60 chromosomes whose lengths ranged from 1.4 to 4.5 µm, and is described by the formula: 4sm+6m+10st+10sm+10m+20sm. The complex of chromosomes from <em>Tradescantia blossfeldiana </em>Mildbr. consisted of 2n = 60. Their length was 1.3-4.8 µm. The morphological types of the chromosomes are represented by the formula 6st+2m+2sm+6st+2sm+12m+10M+l4m+6sm. It is suggested on the basis of morphological analysis of chromosomes that <em>Setereasea purpurea </em>Boom is a tetraploid species and <em>Trudescantia albiflora </em>Kunth. and T. <em>blossfeldiana </em>Mildbr. are decaploids.