scholarly journals MENINGOENCEPHALITIS IN BROILER CHICKS DUE TO ASPERGILLUS FUMIGATUS INFECTION

2021 ◽  
Vol 26 (1) ◽  
pp. 100-108
Author(s):  
H. M. I. AL-Hyali
Keyword(s):  
Aspergillus Fumigatus ◽  
Fibrous Tissue ◽  
Central Area ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Fruiting Bodies ◽  
Histological Sections ◽  
Air Sacs ◽  
Microscopic Demonstration ◽  
Hematoxylin And Eosin

Forty five cases, 7-14 day-old chicks were studied, they showed respiratory and nervous signs with yellowish granulomatous necrotic foci on the air sacs, liver, kidneys, cerebellum and cerebrum. Swabs were taken 7 days post inoculation, culture was made on Sabouraud's dextros agar,. Aspergillus fumigatus was isolated. Diagnosis was confirmed by experimentally exposed one day-old chicks to aerosols of A. fumigatus conidia and also by microscopic demonstration of typical fruiting bodies and spores of the fungus in fresh preparations made from Sabouraud's agar. tissues from fungal-infected Examination of histological sections of cerebrum and cerebellum chicks stained with hematoxylin and eosin and PAS stains revealed granulomas lesion with a central area of necrosis containing heterophils surrounded by macrophages, gaint cells, lymphocytes and layer of fibrous tissue. It was concluded that the cases deal within this study had been infected with A. fumigataus.

scholarly journals Clinical and histopathological study of brooder pneumonia in broiler farms

2014 ◽  
Vol 13 (1) ◽  
pp. 75
Author(s):  
N.R. Abdulrahman
Keyword(s):  
Aspergillus Fumigatus ◽  
Clinical Signs ◽  
First Record ◽  
Giant Cells ◽  
Thoracic Wall ◽  
Watery Diarrhea ◽  
Histopathological Study ◽  
Broiler Chicks ◽  
Air Sacs

The outbreaks of aspergillosis in broiler chicks (4-15 days old) were observed from January to July, 2011 in five broiler farms. The disease was detected on the basis of clinical signs, gross, histopathological and cultural findings. Infected chicks showed signs of ruffled feathers, green watery diarrhea, anorexia, gasping and dyspnea. On examination, numerous small white yellowish nodules were seen in the lungs, air sacs, kidneys, thoracic wall and abdominal serosa. Microscopically the lungs revealed granulomas with central areas of caseation surrounded by heterophils and giant cells. Aspergillus fumigatus could be isolated in Sabouraud’s dextrose agar from the lesions. Higher morbidity (76 to 100%) and mortality (62.5 to 100%) rates were recorded in the five farms. This is the first record on the occurrence of acute aspergillosis in broiler chicks from Sulaimania/ Iraq.

scholarly journals Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia)

2016 ◽  
Vol 37 (4) ◽  
pp. 1919
Author(s):  
Átilla Holanda de Albuquerque ◽  
Régis Siqueira de Castro Teixeira ◽  
Débora Nishi Machado ◽  
Elisângela De Souza Lopes ◽  
Ruben Horn Vasconcelos ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Clinical Signs ◽  
Columba Livia ◽  
Poultry Feed ◽  
Economic Losses ◽  
Control Group ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Microbiological Analysis ◽  
Fecal Samples

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5%) for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%), and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the pathogen in several organs, and simultaneously present diarrheic disorders with significant levels of bacterial excretion in feces.

Acute Airsacculitis in Untreated and Cyclophosphamide-pretreated Broiler Chickens Inoculated with Escherichia coli or Escherichia coli Cell-free Culture Filtrate

1992 ◽  
Vol 29 (1) ◽  
pp. 68-78 ◽  
Author(s):  
M. DeRosa ◽  
M. D. Ficken ◽  
H. J. Barnes
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Culture Filtrate ◽  
Broiler Chickens ◽  
Brain Heart Infusion ◽  
Coli Cell ◽  
Post Inoculation ◽  
E Coli ◽  
Cell Counts ◽  
Air Sacs

Ninety commercial broiler chickens were divided into three equal groups; 30 were injected with brain-heart-infusion broth into the cranial thoracic air sacs (controls), 30 were similarly inoculated with a culture of Escherichia coli, and 30 were similarly inoculated with E. coli cell-free culture filtrate. Birds were examined from 0 to 6 hours post-inoculation. E. coli-inoculated and cell-free culture filtrate-inoculated chickens reacted similarly, with exudation of heterophils into the air sac. Microscopically, heterophils were present in low numbers perivascularly 0.5 hour after inoculation and became more numerous by 3 hours post-inoculation. By 6 hours post-inoculation, there was severe swelling of air sac epithelial cells and thickening of the air sac by proteinaceous fluid and heterophils. Ultrastructurally, air sac epithelial cells were swollen and vacuolated, and interdigitating processes were separated. Histologically and ultrastructurally, all features in control chickens were normal, with only rare heterophils in the air sac interstitium. In E. coli-inoculated and cell-free culture filtrate-inoculated chickens, cell counts (predominantly heterophils) in air sac lavage fluids increased markedly at 3 and 6 hours, with only slight increases in counts from lavages of controls. Heteropenia was observed in E. coli-inoculated chickens, whereas heterophilia was observed in cell-free filtrate chickens and controls. Ninety additional chickens were pretreated with cyclophosphamide, subdivided into three equal groups, and inoculated and examined similarly as above. Cyclophosphamide pretreatment reduced inflammatory changes in air sacs, lowered cell numbers in lavage fluids, and abolished hematologic changes; however, it did not prevent epithelial cell changes. These results indicate that cell-free culture filtrate of E. coli induces changes similar to those induced by cultures of E. coli.

scholarly journals Condrolipoma na orelha de um cão

2017 ◽  
Vol 45 ◽  
pp. 4
Author(s):  
Maria De Fátima De Souza ◽  
Alinne Emanuelle Monteiro Sarmento ◽  
Francisca Maria Sousa Barbosa ◽  
Raul Antunes Silva Siqueira ◽  
Ivia Carmem Talieri ◽  
...  
Keyword(s):  
Alcian Blue ◽  
Periodic Acid ◽  
Fibrous Tissue ◽  
Rare Event ◽  
Periodic Acid Schiff ◽  
Non Invasive ◽  
Pas Staining ◽  
Staining Techniques ◽  
Hematoxylin And Eosin ◽  
Well Differentiated

Background: Lipoma is defined as a tumor composed of mature adipocytes, identical to the cells that form normal adult fat. Other elements may also be present, such as cartilaginous tissue. Chondrolipoma is characterized by the presence of differentiated cartilage islands spread within a typical lipoma, in which mucin and collagen can be observed around isles of chondrocytes. This condition affects mainly middle-aged or elderly dogs as well as elderly humans. In the scientific literature, chondrolipomas have not yet been described on the ear of dogs. Therefore, this study aimed to report a case of chondrolipoma on the ear of a dog and its histopathological and histochemical description.Case: A female, 10-year-old, Labrador retriever dog was admitted to the Veterinarian Hospital of the Federal University of Paraíba (UFPB), Areia, Brazil. The dog was presented with an ulcerated pendulous nodule on the ear, with a clinical evolution of approximately 7 months. A biopsy sample was taken and submitted to the Veterinary Histopathology Laboratory of the UFPB. Macroscopic evaluation revealed a pendulous and firm nodule, with a round apex, measuring 1.3cm in diameter. The epidermis covering the nodule exhibited an ulcer of 0.5cm in length. The nodule exhibited resistance to cutting and the surface was dark red with hard areas in the center. Sample was fixed in 10% formalin and routinely processed for histology, embedded in paraffin, and stained with hematoxylin and eosin (HE), alcian blue (AB), Masson’s trichrome (MT), and periodic acid-Schiff (PAS). Histopathological evaluation revealed that the nodule was encapsulated, formed by adipocytes of variable sizes. Adipocytes were well-differentiated and the nodule was defined as lipoma. Within the lipoma there was proliferation of chondrocyte islands in a bluish basophilic matrix. Adjacently, abundant fibrous connective tissue was found intertwined with blood vessels. Some vessels exhibited red blood cells in the vicinity, indicating hemorrhage. The epidermis covering the nodule was slightly acanthotic and moderately hyperkeratotic. MT staining marked the fibrous tissue in blue. AB staining revealed blue areas confirming the presence of mucin around cartilage islands. PAS staining revealed the presence of glycogen between mesenchymal cells, which included chondrocytes.Discussion: The diagnosis of chondrolipoma was concluded with the presence of a well-differentiated lipoma, randomly intertwined with cartilage islands, which were also well-differentiated and spread within a typical lipoma. Additionally, the presence of mucin and collagen around the isles of chondrocytes facilitated the diagnosis. Chondrocytes presented different phases of maturation. The case described here exhibited microscopic characteristics that were similar but somewhat different to those previously reported by other authors, in considering that the nodule was encapsulated, and did not exhibit cell atypia or mineralization in the cartilage islands. Most studies published used PAS and Masson’s trichrome staining techniques to confirm the diagnosis of chondrolipoma. However, alcian blue also aids in diagnosing this condition. The tumor developed for months before the dog was taken to a veterinarian, when it finally became ulcerated, which characterized it as non-invasive. Chondrolipoma is a benign tumor, which does not induce metastasis and once excised does not exhibit recurrence. In veterinary medicine, the presence of cartilage within a lipoma is a rare event.

scholarly journals Pathomorphological changes in the testes of males with babesiosis

2020 ◽  
Vol 175 ◽  
pp. 03013
Author(s):  
Viktor Mikhailenko ◽  
Olga Dilekova ◽  
Vladimir Meshcheryakov ◽  
Dmitry Chervyakov ◽  
Daniil Proskurin
Keyword(s):  
Sertoli Cells ◽  
Endocrine Cells ◽  
Clinical Signs ◽  
Vacuolar Degeneration ◽  
Histological Sections ◽  
Exfoliated Cells ◽  
Blood Smears ◽  
Prepared Material ◽  
Hematoxylin And Eosin ◽  
Spermatogenic Epithelium

The aim of the work was to study pathomorphological changes in the testes in males with babesiosis. Diagnosis of babesiosis in sick males was performed based on the detection of typical clinical signs and blood smears. Blood smears were fixed in methanol and stained according to the Romanovsky-Giemsa method with hematoxylin and eosin. For histological studies, pieces of testicles were selected, which were fixed in an 8% neutral aqueous formalin solution. The prepared material was poured into paraffin according to the conventional method. Histological sections were stained with hematoxylin and eosin using the method of Van Gieson and Mallory. Pathomorphological examination of stained histological sections revealed changes characteristic of parenchymal orchitis. The spermatogenic epithelium of the convoluted seminal tubules was drained down in all fields of vision. The lumen of the tubules was filled with protein detritus, exfoliated cells of the spermatogenic epithelium and macrophages. Part of the Sertoli cells was in a state of vacuolar degeneration. Focal clusters of lymphoid-macrophage infiltrates were visible between the convoluted seminal tubules, especially multiple ones around the blood vessels. Endocrine cells were subjected to atrophy due to compression by cell infiltrates and connective tissue growths.

Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections

2010 ◽  
Vol 15 (2) ◽  
pp. 026018 ◽  
Author(s):  
Adam Tuer ◽  
Danielle Tokarz ◽  
Nicole Prent ◽  
Richard Cisek ◽  
Jennifer Alami ◽  
...  
Keyword(s):  
Histological Sections ◽  
Hematoxylin And Eosin

scholarly journals Biocompatibility of a Calcium Hydroxide-Propolis Experimental Paste in Rat Subcutaneous Tissue

2014 ◽  
Vol 25 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Graziela Garrido Mori ◽  
Sindineia da Silva Rodrigues ◽  
Sheila Tieko Shibayama ◽  
Marcelo Pomini ◽  
Cristhiane Olivia Ferreira do Amaral
Keyword(s):  
Zinc Oxide ◽  
Connective Tissue ◽  
Calcium Hydroxide ◽  
Inflammatory Process ◽  
Subcutaneous Tissue ◽  
Histological Sections ◽  
Zinc Oxide Eugenol ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Hematoxylin And Eosin

Intracanal medications are fundamental for disinfection of the root canal system and participate in periapical repair, so their biocompatibility is of utmost importance to avoid tissue damage. This study evaluated the biocompatibility of a experimental paste of calcium hydroxide and propolis in the subcutaneous tissue of rats. The study was conducted on 15 male Wistar rats. Two incisions were made on the dorsal region of each animal for introduction of 4 tubes: one tube was empty; one contained zinc oxide-eugenol cement, and the two other tubes were filled with experimental paste. After 7, 14 and 30 days, the animals were euthanized and the specimens were subjected to histotechnical preparation. The hematoxylin and eosin-stained histological sections were analyzed by light microscopy. Scores were established according to the inflammatory process and statistically compared by the Tukey test (α=5%). The analysis of histological sections showed non-significant or mild inflammatory reaction in the connective tissue in contact with the empty tubes in all study periods while the contact of subcutaneous tissue with zinc oxide-eugenol elicited moderate or severe inflammation similarly without significant difference among the study periods. The connective tissue was moderately inflamed at 7 days when contacting the experimental paste, but the inflammatory process was non-significant or mild at 14 and 30 days. The experimental paste was biocompatible with the tissues after 14 days of subcutaneous implantation.

Volume relationships during the pregnancy cycle of the tsetse fly Glossina austeni

1972 ◽  
Vol 50 (7) ◽  
pp. 999-1010 ◽  
Author(s):  
S. S. Tobe ◽  
K. G. Davey
Keyword(s):  
Small Volume ◽  
Tsetse Fly ◽  
Reproductive State ◽  
Volume Changes ◽  
Constant Weight ◽  
Low Level ◽  
Histological Sections ◽  
Glossina Austeni ◽  
A Value ◽  
Air Sacs

Because of their haematophagous and viviparous habits, female Glossina austeni undergo large changes in both volume and weight during each pregnancy cycle. As the developing larva in the uterus increases in size, the volume of the mother also increases. These changes in volume of the adult fly occur almost exclusively in the abdomen, which does not contain significant air sacs with which to buffer the volume changes. The changes in abdominal volume are made possible by an elastic ventral abdominal cuticle which is manifested in histological sections as changes in the thickness of the ventral abdominal cuticle during the pregnancy cycle. Stretching appears to occur in the endocuticle.Female G. austeni tend to feed to a constant weight irrespective of their reproductive state. Before the first ovulation, flies feed to a weight of about 50 mg but subsequently feed to a weight of about 80 mg. This increase coincides with the cessation of postemergence cuticle deposition, as well as ovulation, but it is not known whether these events are functionally related.Haemolymph volume of female G. austeni remains at a low level throughout the observed pregnancy cycles at a value of about 5 μliters. This small volume may impose limitations on the transport of nutrients through the haemolymph. Following feeding, haemolymph volume does not change.

scholarly journals Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary

2020 ◽  
Vol 44 ((E0)) ◽  
pp. 28-32
Author(s):  
Tamara N. Dawood
Keyword(s):  
The Other ◽  
Control Group ◽  
Female Gonad ◽  
Trigonella Foenum Graecum ◽  
Histological Sections ◽  
Vascular Congestion ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Positive Effect ◽  
Histopathological Effects

This study aimed to investigate the impacts of the Trigonella foenum-graecum (T. foenum-graecum) seeds on the female gonad. A total of twenty local rabbits were used in this study; were divided into four groups (5 each): first group (G1) was considered as the control group. The second group (G2), third group (G3) and fourth group (G4) were fed daily1.5%, 3%, and 4.5% of T. foenum-graecum seeds respectively for 60 days (twice daily). At the end of the experiment, the animals were euthanized by diethyl ether (C2H52O). Then the abdomen was incised, and the samples of ovaries were collected and fixed by 10% neutral buffered formalin. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with Hematoxylin and Eosin stain. The result showed that the numbers of primary and secondary follicles were significantly P< 0.05 decreased in G3and G4 compared with the control (G1) and G2. The numbers of Graafian follicles were significantly P<0.05 decreased G4 compared with other groups. The diameters of the primary, secondary, and Graafian follicles were significantly lower than the other groups. The thickness of the granulosa cell layer in G3and G4 were significantly lower than the other groups. The histological figures declared that the ovary of G2 was similar to that in G1. The histological sections of G3 and G4 were revealed marked cortical and medullary vascular congestion and focal hemorrhage; there were also marked follicular degeneration and cystic necrosis. The study concluded that the low concentration of T. foenum-graecum (fenugreek) seeds do not have any positive effect in terms of ovarian stimulation

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