A metrological approach for multispectral photogrammetry

<p>This paper presents the design and development of a three-dimensional reference object for the metrological quality assessment of photogrammetry-based techniques, for application in the cultural heritage field. The reference object was 3D printed, with nominal manufacturing uncertainty of the order of 0.01 mm. The object was realized as a dodecahedron, and in each face, a different pictorial preparation was inserted. The preparations include several pigments, binders, and varnishes, to be representative of the materials and techniques used historically by artists.</p><p>Since the reference object’s shape, size and uncertainty are known, it is possible to use this object as a reference to evaluate the quality of a 3D model from the metric point of view. In particular, verification of dimensional precision and accuracy are performed using the standard deviation on measurements acquired on the reference object and the final 3D model. In addition, the object can be used as a reference for UV-induced Visible Luminescence (UVL) acquisition, being the materials employed UV-fluorescent. Results obtained with visible-reflected and UVL images are presented and discussed.</p>

Remote control of neural function by X-ray-induced scintillation

Scintillators emit visible luminescence when irradiated with X-rays. Given the unlimited tissue penetration of X-rays, the employment of scintillators could enable remote optogenetic control of neural functions at any depth of the brain. Here we show that a yellow-emitting inorganic scintillator, Ce-doped Gd3(Al,Ga)5O12 (Ce:GAGG), can effectively activate red-shifted excitatory and inhibitory opsins, ChRmine and GtACR1, respectively. Using injectable Ce:GAGG microparticles, we successfully activated and inhibited midbrain dopamine neurons in freely moving mice by X-ray irradiation, producing bidirectional modulation of place preference behavior. Ce:GAGG microparticles are non-cytotoxic and biocompatible, allowing for chronic implantation. Pulsed X-ray irradiation at a clinical dose level is sufficient to elicit behavioral changes without reducing the number of radiosensitive cells in the brain and bone marrow. Thus, scintillator-mediated optogenetics enables minimally invasive, wireless control of cellular functions at any tissue depth in living animals, expanding X-ray applications to functional studies of biology and medicine.