Alternative Systems and Materials for Splicing Prestressed-Precast Concrete Piles

The use of piles is a common method for establishing deep foundations for bridges where there is a top layer of weak soil. Among various types of pile and installation methods, driving prestressed-precast concrete piles (PPCP) is a durable and economical option compared with the alternatives. Also, since the method employs pile segments prefabricated in precast plants and delivered to the site for installation, it conforms to the principles of Accelerated Bridge Construction (ABC) and provides a rapid alternative to other methods. However, often because of limitations on shipping and transportation, the length of precast prestressed pile segments that can be delivered to the bridge site has to be reduced. Also, headroom limitations for pile driving may limit the length of pile segments such that establishing adequate resistance may not be achieved with one segment. Therefore, splicing of pile segments has to be performed at the site to produce longer lengths. A study carried out as part of research activities at the Accelerated Bridge Construction University Transportation Center (ABC-UTC) at Florida International University has reviewed various types of available pile splices and attempted to build on the experiences gathered for ABC connections to introduce an alternative configuration for splicing PPCP segments. Accordingly, a variation of grouted bar splice was introduced and designed to provide PPCPs with a time-effective, economical, and labor-friendly method of splicing. The proposed connection is completely new for connecting PPCP segments. Because many of PPCPs are driven in a marine environment, the application of corrosion-resistant material at the splice system is also emphasized. The paper summarizes these investigations. The results of this study show that the newly developed systems can provide the required strength in bending, tension, and compression with smaller sizes and numbers of bars. It also makes the installation faster and easier compared with the current methods.

Antisense Oligonucleotide-Based Rescue of Aberrant Splicing Defects Caused by 15 Pathogenic Variants in ABCA4

The discovery of novel intronic variants in the ABCA4 locus has contributed significantly to solving the missing heritability in Stargardt disease (STGD1). The increasing number of variants affecting pre-mRNA splicing makes ABCA4 a suitable candidate for antisense oligonucleotide (AON)-based splicing modulation therapies. In this study, AON-based splicing modulation was assessed for 15 recently described intronic variants (three near-exon and 12 deep-intronic variants). In total, 26 AONs were designed and tested in vitro using a midigene-based splice system. Overall, partial or complete splicing correction was observed for two variants causing exon elongation and all variants causing pseudoexon inclusion. Together, our results confirm the high potential of AONs for the development of future RNA therapies to correct splicing defects causing STGD1.

Behaviour of Grout-Filled Splice under Monotonic and Cyclic Loads

Grout-filled mechanical splice system is one of the most important technologies for precast construction. However, due to the high cost of grouted sleeve in Chinese construction market, the implementation of this technology is hindered. In this study, a new type of grouted sleeve was developed with standard seamless steel pipe by cold rolling depression. Utilizing this sleeve, eight coupler specimens were prepared and tested under incremental tensile load and cyclic load. The results indicate that all the specimens provided satisfactory behaviour of which the ultimate tensile strength exceeded the required loading capacity specified in ACI318 and JGJ107. The inner cavity configuration of the grouted sleeve has great influence on the confining mechanism of the splice and on the strain distribution in the sleeve. Because the compressive strength of grout is greatly higher than its tensile strength, the slope of the load-strain response curve of the sleeve under tensile load is lower than it under compressive load.