The protist community mirrors seasonality and mesoscale hydrographic features in the oligotrophic Sargasso Sea

Protists represent the majority of the eukaryotic diversity in the oceans. They have different functions in the marine food web, playing essential roles in the biogeochemical cycles. Meanwhile the available data is rich in horizontal and temporal coverage, little is known on their vertical structuring, particularly below the photic zone. The present study applies DNA metabarcoding to samples collected over three years in conjunction with the BATS time-series to assess marine protist communities in the epipelagic and mesopelagic zones. The protist community showed a dynamic seasonality in the epipelagic, responding to hydrographic yearly cycles. Mixotrophic lineages dominated throughout the year; however, autotrophs bloomed during the rapid transition between the winter mixing and the stratified summer, and heterotrophs had their peak at the end of summer, when the base of the thermocline reaches its deepest depth. Below the photic zone, the community, dominated by Rhizaria, is depth-stratified and relatively constant throughout the year, mirroring local hydrographic and biological features such as the oxygen minimum zone. The results suggest a dynamic partitioning of the water column, where the niche vertical position for each community changes throughout the year, likely depending on nutrient availability, the mixed layer depth, and other hydrographic features. Finally, the protist community closely followed mesoscale events (eddies), where the communities mirrored the hydrographic uplift, raising the deeper communities for hundreds of meters, and compressing the communities above.

Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression

Plants use seasonal temperature cues to time the transition to reproduction. In Arabidopsis thaliana, winter cold epigenetically silences the floral repressor locus FLOWERING LOCUS C (FLC) through POLYCOMB REPRESSIVE COMPLEX 2 (PRC2)1. This vernalization process aligns flowering with spring. A prerequisite for silencing is transcriptional downregulation of FLC, but how this occurs in the fluctuating temperature regimes of autumn is unknown2–4. Transcriptional repression correlates with decreased local levels of histone H3 trimethylation at K36 (H3K36me3) and H3 trimethylation at K4 (H3K4me3)5,6, which are deposited during FRIGIDA (FRI)-dependent activation of FLC7–10. Here we show that cold rapidly promotes the formation of FRI nuclear condensates that do not colocalize with an active FLC locus. This correlates with reduced FRI occupancy at the FLC promoter and FLC repression. Warm temperature spikes reverse this process, buffering FLC shutdown to prevent premature flowering. The accumulation of condensates in the cold is affected by specific co-transcriptional regulators and cold induction of a specific isoform of the antisense RNA COOLAIR5,11. Our work describes the dynamic partitioning of a transcriptional activator conferring plasticity in response to natural temperature fluctuations, thus enabling plants to effectively monitor seasonal progression.

Numerical Study of the Effect of Perforation Friction and Engineering Parameters on Multicluster Fracturing in Horizontal Wells

Simultaneous multiple-fracture treatments in horizontal wellbores have become one of the key methods for economically and efficiently developing oil and gas resources in unconventional reservoirs. However, field data show that some perforation clusters have difficulty propagating fractures due to the internal mechanism of competing initiation and propagation among the fractures. In this paper, the physical mechanisms that influence simultaneous multiple-fracture initiation and propagation are investigated, and the effects of engineering parameters and in situ conditions on the nonuniform development of multiple fractures are discussed. A 3D fracture propagation model was established with ABAQUS to show the influence of the stress shadow effects and dynamic partitioning of the flow rate by simulating the propagation of multiple competing fractures generated in the perforation clusters. Based on the results of these simulations, simultaneous flow in multiple fractures can propagate evenly. Through adjusting the number of perforations in each cluster or the perforation diameter, the effect of the stress shadow can be significantly reduced by increasing the perforation friction, and the factors that affect the development of multiple fractures are changed, from the stress shadow effect to the dynamic partitioning of the flow rate. When the stress shadow effect is dominant, increasing the fracturing fluid viscosity promotes the uniform development of multiple fractures and increases the fracture width. When the dynamic partitioning of the flow rate is dominant, increasing the injection rate greatly affects the uniform development of multiple fractures.