Harmony Search Algorithm for Minimizing Assembly Variation in Non-linear Assembly

The proposed work aims to acquire the maximum number of non-linear assemblies with closer assembly tolerance specifications by mating the different bins’ components. Before that, the components are classified based on the range of tolerance values and grouped into different bins. Further, the manufacturing process of the components is selected from the given and known alternative processes. It is incredibly tedious to obtain the best combinations of bins and the best process together. Hence, a novel approach using the combination of the univariate search method and the harmony search algorithm is proposed in this work. Overrunning clutch assembly is taken as an example. The components of overrunning clutch assembly are manufactured with a wide tolerance value using the best process selected from the given alternatives by the univariate search method. Further, the manufactured components are grouped into three to nine bins. A combination of the best bins is obtained for the various assembly specifications by implementing the harmony search algorithm. The efficacy of the proposed method is demonstrated by showing 24.9% of cost-savings while making overrunning clutch assembly compared with the existing method. The efficacy of the proposed method is demonstrated by showing 24.9% of cost-savings while making overrunning clutch assembly compared with the existing method. The results show that the contribution of the proposed novel methodology is legitimate in solving selective assembly problems.

Precursor Linear Self-Assembly Nonhydrolytic Sol-Gel Synthesis of Ba0.7Sr0.3TiO3 Ceramic Fibers

Ba0.7Sr0.3TiO3 ceramic fibers were synthesized via the precursor linear self-assembly nonhydrolytic sol-gel (NHSG) method, taking TiCl4 as the titanium source, anhydrous barium acetate and strontium acetate as the barium source and strontium source, anhydrous ethanol and glycol as the oxygen donor and solvent, respectively. The NHSG method promotes the formation of Ba–O–Ti and Sr–O–Ti through heterogeneous condensation. The bimolecular association structure of the reaction intermediate (chlorotitanium ethoxide) between ethanol and titanium tetrachloride facilitates the self-linear assembly of precursors. It also enables linear colloidal particle formation and excellent spinnability of the sol. The novel Ba0.7Sr0.3TiO3 ceramic fibers would promote the flexibility of electronic products.

Minimizing Cost of Assembly of an Interrelated Dimensional Chain Product Using ABC Algorithm

Selective assembly is a method where components made with wider tolerance are grouped into a number of bins. Based on the best combination of the bin, the corresponding group components are randomly selected and matched together to make an assembly. Existing techniques focused on equal group number partitioning of components, equal probability, equal group width, and equal area methods to minimize either clearance variation or surplus parts using different optimization techniques. Mostly, simple assemblies with two or three components are worked by various authors in the literature without considering their original dimension by considering only their component’s tolerance. In the present work, components are classified into different unequal group numbers based on their tolerance values. The interrelated dimensional assemblies are made in a single stage by matching the parts based on the best bin combination obtained by the artificial bee colony algorithm. A simple linear assembly and a three-armed knuckle joint assembly are considered examples of problems to demonstrate the effectiveness of the proposed method by minimizing the manufacturing cost.

The structure, function, and evolution of a complete human chromosome 8

The complete assembly of each human chromosome is essential for understanding human biology and evolution. Using complementary long-read sequencing technologies, we complete the first linear assembly of a human autosome, chromosome 8. Our assembly resolves the sequence of five previously long-standing gaps, including a 2.08 Mbp centromeric α-satellite array, a 644 kbp defensin copy number polymorphism important for disease risk, and an 863 kbp variable number tandem repeat at chromosome 8q21.2 that can function as a neocentromere. We show that the centromeric α-satellite array is generally methylated except for a 73 kbp hypomethylated region of diverse higher-order α-satellite enriched with CENP-A nucleosomes, consistent with the location of the kinetochore. Using a dual long-read sequencing approach, we complete the assembly of the orthologous chromosome 8 centromeric regions in chimpanzee, orangutan, and macaque for the first time to reconstruct its evolutionary history. Comparative and phylogenetic analyses show that the higher-order α-satellite structure evolved specifically in the great ape ancestor, and the centromeric region evolved with a layered symmetry, with more ancient higher-order repeats located at the periphery adjacent to monomeric α-satellites. We estimate that the mutation rate of centromeric satellite DNA is accelerated at least 2.2-fold, and this acceleration extends beyond the higher-order α-satellite into the flanking sequence.

The structure, function, and evolution of a complete human chromosome 8

ABSTRACTThe complete assembly of each human chromosome is essential for understanding human biology and evolution. Using complementary long-read sequencing technologies, we complete the first linear assembly of a human autosome, chromosome 8. Our assembly resolves the sequence of five previously long-standing gaps, including a 2.08 Mbp centromeric α-satellite array, a 644 kbp defensin copy number polymorphism important for disease risk, and an 863 kbp variable number tandem repeat at chromosome 8q21.2 that can function as a neocentromere. We show that the centromeric α-satellite array is generally methylated except for a 73 kbp hypomethylated region of diverse higher-order α-satellite enriched with CENP-A nucleosomes, consistent with the location of the kinetochore. Using a dual long-read sequencing approach, we complete the assembly of the orthologous chromosome 8 centromeric regions in chimpanzee, orangutan, and macaque for the first time to reconstruct its evolutionary history. Comparative and phylogenetic analyses show that the higher-order α-satellite structure evolved specifically in the great ape ancestor, and the centromeric region evolved with a layered symmetry, with more ancient higher-order repeats located at the periphery adjacent to monomeric α-satellites. We estimate that the mutation rate of centromeric satellite DNA is accelerated at least 2.2-fold, and this acceleration extends beyond the higher-order α-satellite into the flanking sequence.

Synergistic Path for Dual Anisotropic and Electrically Switchable Emission From a Nanocomposite of CsPbBr3 Quantum Cuboids and Nematic Liquid Crystal

We report photophysical properties of a nanocomposite consisting of perovskite quantum cuboids (QCs) formed by CsPbBr3 and a wide temperature range nematic liquid crystal. Contrary to observations made with conventional II-VI quantum dots dispersed in a liquid crystal, the used QCs form, under the influence of the nematic orientation, linear assemblies over macroscopic length scales evidenced by polarizing optical microscopy. Interestingly, the linear assembly is actually caused by such an anisotropic arrangement at the nm scale, as seen in TEM images. Thin films of the nanocomposite exhibiting this unique and fascinating character exhibit absorption and emission features, which are quite appealing. These include retention of the sharp bandwidth of emission characteristic of the native QCs and establishment of dual anisotropies, arising from the values being different along the director as well in the two directions orthogonal to it. We also present data on voltage-driven switching between one of the anisotropic limits.